冰冻切片

Two hours later, the convulsant mice were anesthetized deeply and fixed by transcardiac perfusion for Immunohistochemistry to detect c-Fos expression.③ Comparison of neurotoxicity induced by CD50 of three agents.

将发生惊厥的小鼠妥善标记，2 h后制作冰冻冠状切片，检测神经元核蛋白c-Fos的表达，镜下计算不同区域表达c-Fos的棕黄色颗粒数目，每个颗粒代表1个神经元。

Objective The origin and the nature of the Hodgkin and Reed-Sternberg cell of Hodgkins lymphoma has been attracting a lot of medical researchers engaged in studying it,for its character by scattered large atypical cells residing in a complex admixture of inflammatory cells.Great improvement has been made since a new method of isolation of single H/RS cells from a frozen section had been set up by KUppers in 1994,and many studies have approved of the B-cell derivation of H/RS cell.lt has been reported that H/R-S cells might partly be originated from B-cell in our research before,but at the same time,we also found that only 18.8% of H/RS cells express CD20,31.3% of immunoglobulin heavy chain rearrangement have been revealed.

目的 霍奇金淋巴瘤(Hodgkins lymphoma，HL)由于它的恶性肿瘤细胞—H／RS细胞一般只占肿瘤组织的极少部分(不到1％)，且散在分布在背景细胞间，因此对于H／RS细胞的来源和性质研究一直是人们探索的目标。1994年德国科隆大学Kūppers等发展了一种从冰冻组织切片上用显微操作仪挑取单个H／RS细胞的显微切割方法进行H／RS细胞基因分析后，人们对H／RS细胞来源的研究有了突破性的进展，多数支持B细胞来源。

The nerve innervation of the cornea was observed in this histological section under microscope.

利用氯化金神经染色和冰冻连续切片技术，在光学显微镜下研究了23w和33w人胎角膜的神经分布。

Methods Healthy male SD rats of different age groups were recruited(1-, 2- and 3- week groups, 1- and 2- month groups, 6- month grown group, and 18- month aged group; n=5 each). Immunohistochemistry was performed on the coronal sections of brain of the rats perfused with paraformaldehyde.

健康雄性SD大鼠，分为7天、2周、3周、1月、2月、成年（6月龄）和老年（18月龄）7组，每组5只，共35只，多聚甲醛灌注固定，冰冻冠状切片，免疫组织化学染色，光镜观察摄像，细胞计数。

Methods The skeletal muscle specimens with obviously regenerating muscle fibers were obtained from 9 patients with pseudohypertrophic muscular dystrophy and 5 patients with polymyositis. Then frozen serial sections were made and the sections were treated with HE stain, immunohistochemistry stains including antiEzrin and antiNeural cell adhesion molecule monoclonal antibodies. The pathological changes and the expression of Ezrin were observed.

取肌纤维再生活跃的假肥大型肌营养不良（DMD，9例）和多发性肌炎（PM，5例）患者的骨骼肌标本，冰冻连续切片，进行HE染色及抗Ezrin、抗神经细胞黏附分子单克隆抗体免疫组化染色，观察被检肌的病理改变和Ezrin蛋白的表达。

However, most of GSK3βand E2F1 translocated into the nuclear and led to the stronger interaction in nuclei after the PC12 cells were stimulated by NGF.

用小鼠的海马区的冰冻切片进行免疫荧光实验，发现在成熟的神经元中，GSK3β与E2F1都定位在细胞质中。

Furthermore there are more apoptosis cells in inner root sheath than in outer root sheath. Conclusion When we culture human hair follicle in vitro, Williams E serum-free medium is a suitable choice.This model is a fine model also, which is used for screening drug that may accelerate or inhibit the hair growth.Cryo-section can simplify procedure of paraffin section in histology detection of culture human hair follicle in vitro.We can observe the ultrastructural change of human hair follicle in vitro by transmission electron microscope,which can assist to observe apoptosis cells. The eyepiece micrometer is a simple tool for measuring length of hair follicle in vitro.

在离体培养人头皮毛囊时，Williams E无血清培养基是合适的选择，该模型也是筛选促进或抑制毛发生长药物的良好模型；离体培养毛囊组织学检测用冰冻切片可以简化步骤；透射电镜可以用来观察离体培养的毛囊的形态和超微结构的变化，也可以用来协助观察细胞凋亡的状况。

Methods Fifteen healthy, female SD rats were employed in the experiment. Sensitized systematically with keyhole limpet hemocyanin, the animals were inoculated with the same antigen through cochlea basal turn into the labyrinth. Adminstrated 5-bromo-2′-deoxyuridine intraperitoneally, the rats were sacrificed and the temporal bones were harvested at 3, 7,14 day after labyrinth vaccination respectively. The frozen sections of the decalcified samples were dealt with H-E staining and immunohistohemical methods to investigate the cellular infiltration, BrdUrd and IgG positive cells in the ES.

选用SD大鼠15只，以钥孔虫戚血蓝蛋白（keyhole limpet hemocyanin，KLH）全身免疫后，经耳蜗底回钻孔以相同抗原进行内耳免疫，然后分别在内耳免疫后3、7和14 d腹腔注射溴脱氧尿嘧啶核苷（bromodeoxyuridine，BrdUrd）后处死动物，取颞骨经组织学处理制作冰冻切片，用免疫组织化学技术观察内淋巴囊的细胞浸润、增殖和IgG细胞的分布状况。

Methods: The expression of TLR4 was determined by Immunohistochemistry in 20 human pancreases.

采用免疫组化方法观察20例正常人胰腺组织冰冻切片TLR4的表达分布情况。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。