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冰冻切片

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3PH was done in group M and T. Then the rats in B group and M group were injected normal saline via portal vein, and those in T group were injected with SHCs suspension. Four week later, the level of serous alanine aminotransferaseand glutamic oxalacetic transaminase were tested, and liver pathological changes observed. Frozen sections of T group were observed under fluorescent microscope.

将实验用同一近交系SD大鼠30只随机平均分成空白对照组、损伤造模组和SHCs移植组。B组腹腔注射生理盐水,M组、T组腹腔注射CCl4.24 h后戊巴比妥腹腔麻醉,消毒开腹,M、T组行2/3肝切,B、M组门静脉注入生理盐水,T组门静脉注入经膜荧光标记SHCs悬液。4周后,采血测血清谷丙转氨酶、谷草转氨酶,并取病理标本做冰冻切片荧光显微镜下观察及HE染色普通光镜下观察。

Of 48 (16 7%) cases with paragonimiasis showed positive cross reaction.

幼虫冰冻切片的抗原活性于-20℃可保存至5年。

It is necessary to give frozen section examination in parotidectomy.

实施腮腺手术时,术中行冰冻切片检查是必要的。

When I first pasted the frozen photos here I did not know it would be so complicated case.

当我最初上传冰冻切片时,我不知道它会如此复杂

Microbubbles attached with the naked plasmid DNA of EGFP were infused into the rat by periocular injection with destructing microbubbles by ultrasound immediately. After 2 weeks , the EGFP expression in the rats'retina were detected by confocal laser scanning microscopy .

两周后处死大鼠做冰冻切片,在激光共聚焦显微镜下观察蛋白表达情况及分析EGFP质粒在大鼠色素上皮层的表达强度。

The microtubule cytoskeleton was investigated by using cryosectioning and indirect immunofluorescence staining in the shoot apex of Saccharum spontaneum.

利用冰冻切片法结合间接免疫荧光标记技术对割手密茎尖细胞有丝分裂过程中微管骨架的变化进行了研究。

GFP cells were observed in bone marrow of irradiated animals and unirradiated animals 24 h after transplantation.

于移植后几个时间点取脑、骨髓、肝、脾等做冰冻切片,用双光子共聚焦红外脉冲激光显微镜检测GFP-MSCs。

The quantity and appearance of Foslike immunopositive neurons in ventrolateral part of PAG were investigated by immunocytochemical staining method.

采用免疫组织化学ABC法对大鼠中脑冰冻切片进行染色,显微镜下进行观察并拍照,对Fos免疫阳性细胞进行形态与计数分析。

Then the animals were sacrificed and the surface preparation and the section of cochleae stained by MyosinⅥwere observed.Other cochlea were observed with SEM.

分别于术后第3.5个月分别行听性脑干反应检查后取双侧耳蜗标本做基底膜铺片和耳蜗冰冻切片MyosinⅥ染色观察有无新生毛细胞样细胞出现,部分耳蜗作扫描电镜观察。

Total proteins were isolated from retinas of experimental and control eyes and were assessed by Western blot analysis.

将眼球固定后制成14μm厚的冰冻切片进行Ets-1和VEGF免疫荧光双标检测。

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