冰冻切片

RESULTS: Frozen section of pancreas in group A didn't appear yellow-green fluorescence.

结果： A组的胰腺冰冻切片未见有黄绿色荧光。

HRP was coupled with the fluorochrome FITC and encapsulated with liposome . pEGFP-N1 plasmid (expressing green fluorescent protein) with encapsulation in commercially available liposome was prepared. The eyes were enucleated 1, 4 and 8 weeks after zonule rupture and anterior segments comprising lenses were incubated in medium containing one of these components. Cryo-sections were made and translocation of fluorescent macromolecule from the medium into the lens and green fluorescent protein expression in the epithelium were observed by fluorescent microscopy.

制备FITC标记的HRP脂质体；制备pEGFP-N1质粒（表达绿色荧光蛋白的质粒），并用脂质体包裹；豚鼠悬韧带部分离断后1周、4周、8周取含晶状体的眼前段标本分别在含有FITC-HRP复合物、pEGFP-N1质粒的培养基中孵育，冰冻切片，荧光显微镜观察FITC-HRP复合物进入晶状体和pEGFP-N1质粒在晶状体内表达的情况，比较悬韧带离断侧与非离断侧的差异。3。

Then sacrificed the rats in every group to get cornu dorsale medullae spinalis and to make frozen section.The changes of orphanin receptor mRNA which was related with algesia in cornu dorsale medullae spinalis were observed by hybridization in situ.

各组动物处死后，取脊髓背角组织，冰冻切片，采用原位杂交的方法，观察各组脊髓背角Ⅰ～Ⅵ层内与痛觉相关孤啡肽受体mRNA的变化。

After heart perfusion, the spinal cord and brainstem were removed, and post-fixed overnight, then coronally sliced into 25μm thick of each piece.

取出后过量戊巴比妥钠麻醉，心脏灌流，取脊髓和脑干，后固定过夜，冰冻切片，片厚25μm。

We observed testes, epididymis, ovaries, oviducts and uteri in six species of microbats by the Haematoxylin and Eosin staining of frozen tissue sections and found that sperm storage in five of the 6 species.

采用冰冻切片和苏木精－伊红染色法，观察了6种蝙蝠的睾丸、附睾、卵巢、输卵管和子宫，发现5种蝙蝠有精子储存现象。

Objective To research feasibility and clinical value of high-speed frozen section histodiagnosis for breast puncture tissue.

目的 探讨对超声引导下穿刺的乳腺组织进行快速冰冻切片检查、以得到病理学诊断的可行性和临床应用价值。

MATERIALS AND METHODS: hMSCs labeled with enhanced green fluorescent protein were transplanted into the lateral ventricle of neonatal mouse brain. At 0, 9 and 14 days post-transplantation, MICE were sacrificed and their brains were fixed with 4% paraformaldehyde. The engraftment of transplanted cells in coronal section of the recipient mouse brain was examined wider a fluorescent microscope. Indirected immunofluorescent staining was used to detect the expression of neural proteins of these grafted cells.

材料与方法：将标记绿色荧光蛋白的人骨髓间充质于细胞植入到新生3d的小鼠侧脑室中，分别于移植后0d，9d和14d处死受休鼠，取其脑组织，4%多聚甲醛固定后行冠状面冰冻切片，荧光显微镜下观察hMSCs的植入，激光共聚焦显微镜检测植入细胞神经特异性蛋白的表达，定位双重标记的植入细胞。

Depending on the frost slice pathology,a single lactiferous duct system and its regional excision is the only reliable procedure in establishing a certain diagnosis and controlling thedischarge.

溢液涂片脱落细胞学、红外线及B超对乳头溢液的病因诊断帮助不很大，术中冰冻切片是唯一确诊和决定术式的方法。

Guided by methylene blue,single lactiferous duct system and its regional excision were done first,then decided the further different operation by frost slice pathology.

术中经溢液乳管注入美蓝染色，先行病变乳管和所属腺体区段切除，再根据术中冰冻切片结果决定进一步手术方式。

Dr. Tezelman and colleagues conclude that the accuracy of DCE-MRI in the detection of thyroid carcinoma in patients with multinodular goiter was higher than that of both FNAB and FS analysis.

Tezelman教授和他的同事们得出这样的结论，DEC-MRI在发现多发性甲状腺节结中的甲状腺恶性肿瘤比细针穿刺和冰冻切片。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。