冰冻切片

Methods:Twenty two cadeveric skulls of adults were chosen and made into continuous slices by using the technique of celloidin embedding.

冰冻切片技术是将标本低温冷冻后用电动带锯制作断层切片的一种技术。

The freezing slices of brain tissue were made, which different locations were collected from the brains of 12 catties. Then using the antiserum as the first antiboty and the monoclonal antibody 4C11 as the collator, the slices digested or undigested by protease K were dyed by ABC dyeing. The result was used to determined the location of PrP in cattle brain.

以12健康头黄牛脑组织为研究对象，采取脑组织的不同部位制成冰冻切片，以自制抗血清为一抗和单抗4C11为参照，应用ABC染色法对蛋白酶K处理的和未作处理的切片染色，检测牛脑组织中朊蛋白的分布。

Methods Cryostat sections for adult worms and paraffin sections for eggs in the liver of infected mouse, sporocysts and cercariae in snails were prepared, immunofluorescent test was performed to detect the presence of iNOS in adult worms, sporocysts, cercariae and miracidium, the distribution of this enzyme was observed in different stages of Schistosoma japonicum. Western blotting was used to further demonstrate the immunoreactivity to iNOS in adult worms.

收集日本血吸虫成虫制成冰冻切片，将含胞蚴、尾蚴的感染性钉螺和含成熟虫卵的小鼠肝脏制成石蜡切片，用免疫荧光法检测成虫、胞蚴、尾蚴以及毛蚴中的iNOS，并观察该酶在各期虫体内的分布；用蛋白质印迹法检测成虫匀浆中的可溶性蛋白是否具抗iNOS多抗的可溶性蛋白。

There were no false positive reports among the 220 (40.1%) biopsies interpreted as benign lesions in paraffin sections. Among 329 (59.9%) malignant biopsies on paraffin sections, 3 cases were interpreted as benign lesions on frozen sections. Three false negatives included 2 ductal carcinoma in situ and one infiltrating ductal carcinoma associated with papillomatosis. The tumors were small and confined to the breast without any evidence of metastasis.

本研究针对五年来本院 549 例乳房病理冰冻切片及永久性石蜡病理切片诊断比较，发现在 220 例的良性乳房病变40.1%）并未发现任何假阳性的冰冻病理切片诊断，而在 329 例（ 59.9 %）恶性乳房病变中，其中有三例假阴性包含二例乳房管道原位癌及一例合并良性乳突瘤的浸润性乳癌，而这三例均属较小病变及未有腋下淋巴腺转移。

There were inflammatory cells infiltrating in the myocardium sparely and no calcification in the pericardium in IVIG and IVIG+VitC groups. The size of spleens enlarged especially in IVIG and IVIG+VitC groups.

心脏分为三部分：一部分作病理切片和H-E染色，一部分作冰冻切片，在荧光显微镜下用直接免疫荧光法测定心肌组织中沉积的IgG水平，一部分作电镜检查。

A firm tumor was identified adjoining the capsule of the superficial lobe of the parotid gland. Both frozen section and pathologic reports showed histiocytic necrotizing lymphadenitis.

术中发现紧邻腮腺浅叶被膜有一坚实、大小3×3cm的肿瘤，冰冻切片及永久切片报告均为组织球性坏死性淋巴结炎。

The 28 species of Cycas debaoensis, Stangeria eriopus and Macrozamia moorei etc.in 3 families of Cycadopsida were carried out anatomical studies on vascular tissue, the roots ,stems and leaves of these materials were made histological section by paraffin method and freeze method ,light microscopy observation and the dissociated materials of every organ for light microscopy and scanning electron microscopy observation research.

本探究对苏铁纲3个科的德保苏铁、托叶铁、摩瑞大泽米等28个苏铁种类进行了维管组织的解剖学研究，其中对其根、茎、叶进行石蜡切片和冰冻切片观察研究；对各器官材料进行组织离析，光学显微镜和扫描电子显微镜观察研究。

58 patients with parotid masses in our department from 1991 to 1999 were given frozen section biopsy in the during of parotidectomy and were analyzed on the clinical diagnosis and frozen section results compared with the final histologic diagnosis.

对1991～1999年间住院的58例腮腺肿块病例，术中进行冰冻切片检查，分析临床诊断和冰冻切片检查与最后病理诊断的符合率。

At the end of 12 and 18 weeks, rats were killed, taken the muscle of posterior limb, after separating the gastroenemius、soleus、plantaris, to prepare the frozen section of the each kind of muscle respectively to be confirmed to form the myopathy model by the histochemical stain.(3) RT-PCR、Western blot and immunohisto-chemistry stain were respectively used to detect the expression of NF-κB、MMP-2 and MMP-9 during different period.

2实验组用酒精给大鼠灌胃，对照组用含与酒精体积相等的生理盐水灌胃，两组均喂饲改良的全营养高脂饲料，于12周及18周后，取后肢肌肉，在分离出腓肠肌、比目鱼肌、跖肌后，将各类肌肉均分别地制备成冰冻切片和石蜡切片，经组织化学染色确认成模。

On the frozen sections, the smear preparations, the paraffin sections and the desquamated epithelia samples,various antigens in eight kinds of tissues and cells were demonstrated at the light and electron microscopic levels by using the HRP-SPA immunohistochemical method,and being compared with the PAP method.

应用HRP—SPA免疫组化方法，并对比PAP法，在冰冻切片、细胞悬液涂片、石蜡切片和脱落细胞涂片上，在光镜及电镜水平上观察了八种组织细胞内的不同抗原物质。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了