冰冻

However, most of GSK3βand E2F1 translocated into the nuclear and led to the stronger interaction in nuclei after the PC12 cells were stimulated by NGF.

用小鼠的海马区的冰冻切片进行免疫荧光实验，发现在成熟的神经元中，GSK3β与E2F1都定位在细胞质中。

There are very very very few people like me willing to push the limit in such a fashion. I vividly remember once I held a camcorder and video-taped for two minutes in freezing condition while skiing at high speed. My fingers were so frozen that I had to go straight to the ski lodge in order to warm them up.

非常少的人会像我一样，愿意用这样的方式来推向极限，我清楚记得有一次，我在高速滑雪时，握著摄影机在冰冻的情况下录影二分钟，我的手指头都弄冻僵了，而必需直奔到滑雪小屋将它们弄暖。

Go all out, open the first family after the war, both sides are pushing to the central site of the Battle Flag, which is both the first leg of the contest, this time should be such as "Behind Mirage","reflection of the setting sun,""Emergency,"" nodules frozen "and other high-performance skills without stint, without the use of reserves to achieve the victory of the first leg, head-to-head wins brave, be sure to overwhelm the opponent from the momentum!

全力以赴、打开开局家族战开始后，双方全力拼抢场地中央的战旗，这是双方首回合的较量，这个时候应把诸如&幻影迷踪&、&回光返照&、&冲锋&、&冰冻结节&等高效技能毫不吝惜、毫不保留地运用上，力求取得首回合的胜利，狭路相逢勇者胜，务必要从气势上压倒对手！

The smart mage polymorphs me, frost novas my cat and insta pyroblasts it, or some ridiculously damaging move that kills my pet, thus forcing me to battle it range on range, damage for damage, and that normally hurts a lot.

聪明的法师会把我变羊，冰冻PET，然后用技能搞掉PET，强迫我做1V1的攻击，但那并没多大意义。

At last she became the blasting fuse of all the tragedies.All innocent people were involved in this revenge,and their love and symptoms of human natures were frozen at that time.

最终凯瑟琳成为所有悲剧的导火线，每一个原本纯真的人都被卷了进来，他们的爱和人性都在那个时刻被冰冻了起来。

All experiment animals lived well with no death or malignant arrhythmia following cell transplantation. Frozen sections of the heart showed a few blue-stained cells under the fluoroscope in the cell transplantation group 4 weeks later. This indicated that stem cells were successfully homing. Using hematoxylin-eosin staining, homing cells were distributed over the infarct zone, with the presence of single or mass. In the model control group, no blue-stained cells were determined.

细胞移植后两组大鼠均存活良好，未出现死亡及恶性心律失常，4周后细胞移植组心脏冰冻切片荧光镜检显示心肌内有少量胞核蓝染的细胞，提示干细胞成功归巢，结合苏木精-伊红染色，归巢细胞位于心肌梗死灶周边区域，呈单个或小团聚集；而模型对照组心肌切片未见胞核蓝染的细胞。

Prepare crumble. Rub-in the cold butter with flour and mix like bread crumb.

先制作金宝部份，将冰冻牛油与麫粉用手指搓成麫包糠状。

Wed take the train into the city and Id hire a taxi cab to take us to a fine restaurant. Id order two steak dinners and a glass of French wine for Margaret and an ice-cold bottle of beer for me. Once, when I hit on a quarter bet, I also bought her flowers.

当我中了奖时，我常和玛葛瑞特一起庆祝，有时我俩搭火车去旅行，有时我俩租一辆车去餐馆，我点两份牛排，为玛葛瑞特点一杯法国葡萄酒，自己则点一瓶冰冻啤酒。

The feed must clean, fresh, prohibit that feeds feed which gets moldy and spoil, freezes, simultaneously must pay attention to foreign matter and so on iron wire which, glass, grit mixes with except the feed, after preventing the cattle and sheep pass the food, to have diseases and so on reticulum pericarditis.

饲料要清洁、新鲜、切忌饲喂霉变、冰冻的饲料，同时要注意除去饲料中以夹杂的铁丝、玻璃、沙石等异物，防止牛羊通食后发生网胃性心包炎等疾病。

In order to research to disease of cement concrete pavement and its use circumstances effectively, the writer developed disease data-base for cement concrete pavement of seasonal frost zone and pavement evaluation system.

为了更有效的研究水泥混凝土路面的病害和使用情况，笔者开发的季节性冰冻地区水泥混凝土路面病害数据库及路面检评系统，本部分内容介绍了该系统的功能和系统的实现方法。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。