冰冻

The NPC's will also get frozen and, if you are using them, must be unfrozen as

NPC也是会被冰冻的，如果你要使用他们，那请尽快帮他们解冻

It contains trace amounts of water vapor in its atmosphere and large caps of frozen water at its poles.

在它的大气中包含了水蒸气的痕量，在它的两极覆盖了大量的冰冻水。

He had travelled by yak and on foot; he was now verminous and starving, in rags of sheepskin, crippled with sciatica from sleeping on frozen ground, and without a rupee to his name. But gold shone ahead of him.

他靠牦牛和步行完成长途跋涉，得了寄生虫病，而且饿得发昏，身上披着破碎的羊皮，由于长久在冰冻的地面睡觉而产生的坐骨神经痛使他走路时一瘸一拐，他身上一分钱都没有，而现在，黄金在他面前闪烁。

Canadian ice wine is made from the hundreds of years old German grapes through a very delicate method whose intricacy vindicates its high value. When it comes to harvest season, the viniculture farmers would leave the grapes exposed to snow and frost rather than picking them down the baskets till the next year when the temperature is below 15℃ and all the grape juice is solidified to clot, then they pick the grapes manually in a paced manner. The grapes picked in this way are very sweet. The percentage of sugar is increased to 45% in the 600 liters grape juice extracted from the 1000 liters grapes. With the 600 liters juice, only about 150 liters ice wine can be made.

加拿大的冰酒是由已有百年历史的德国葡萄品种所酿制，在葡萄收成季节时先不予摘收，任其在葡萄树上遭受冰霜的侵袭，直到次年温度低于摄氏15度时，葡萄结霜、冰冻到汁液全都凝固，再以手工快速摘取，此时的葡萄甜度高，约1000公斤的葡萄，只能产出600公升的葡萄汁，其糖份提高至45%，而这600公升的葡萄汁最后只可以制成约150公升的冰酒，所以冰酒之珍贵，不无道理。

I wanta bottle of iced Coca cola .

我要一瓶冰冻可口可乐。

I had a kind of feeling that I was about as popular with him as a cold Welsh rabbit.

我有种感觉，我对他来说不会有趣过冰冻威尔士兔。

In view of the current market, most of the whitebait processing was only stay in freeze drying, so few reports were on its deep processing.

目前，银鱼的加工方式大部分停留于冰冻保藏，有关其深加工的报道很少。

RESULTS: Frozen section of pancreas in group A didn't appear yellow-green fluorescence.

结果： A组的胰腺冰冻切片未见有黄绿色荧光。

HRP was coupled with the fluorochrome FITC and encapsulated with liposome . pEGFP-N1 plasmid (expressing green fluorescent protein) with encapsulation in commercially available liposome was prepared. The eyes were enucleated 1, 4 and 8 weeks after zonule rupture and anterior segments comprising lenses were incubated in medium containing one of these components. Cryo-sections were made and translocation of fluorescent macromolecule from the medium into the lens and green fluorescent protein expression in the epithelium were observed by fluorescent microscopy.

制备FITC标记的HRP脂质体；制备pEGFP-N1质粒（表达绿色荧光蛋白的质粒），并用脂质体包裹；豚鼠悬韧带部分离断后1周、4周、8周取含晶状体的眼前段标本分别在含有FITC-HRP复合物、pEGFP-N1质粒的培养基中孵育，冰冻切片，荧光显微镜观察FITC-HRP复合物进入晶状体和pEGFP-N1质粒在晶状体内表达的情况，比较悬韧带离断侧与非离断侧的差异。3。

Methods:(1) ADM was produced from swine skins treated with trypsin followed by Triton X-100.(2) type I collagen of mouse tail was extracted by 0.5mol/L acetoacetic acid.(3) Two kinds of ADM were got by being soaked with 0.02% glutaraldehyde for 5~10 min or being soaked with mouse type I collagen for 24h, then preserved at 4℃.

(1)应用胰蛋白酶消化-去污剂法制备ADM；(2)用0.5mol/L乙酸溶液提取制备I型鼠尾胶原；(3)用0.02%戊二醛溶液浸泡ADM5-10min，制成交联型ADM；另一部分ADM用质量分数0.25%的I型鼠尾胶原浸泡24h，制成胶原包埋型ADM，冰冻保存；(4)用酶消化法培养原代成纤维细胞，取第三代增殖期细胞，将其调整至浓度为2×105/ml的成纤维细胞悬液。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。