冬虫夏草

In general, our prices are Tong Ren Tang Chinese caterpillar fungus Cordyceps price of 50%, which is common in pharmacies and hospitals, the price of Cordyceps about 65%, so you only need to see our release of Cordyceps sinensis prices, will know the market price trends.

一般来说，我们的冬虫夏草价格是同仁堂冬虫夏草的价格的50%，是普通药房和医院冬虫夏草的价格的65%左右，所以，你只需要查看我们发布的冬虫夏草价格，就会知道市场的价格趋势。

Presently myself according to many years and join "the chinese pharmacopoeia" to the chinese native medicine understanding to do to the dong chon xia cao by the brief analysis,enhances us to dong chon xia cao's further understanding.

现本人根据多年对中药的认识和接合《中国药典》对冬虫夏草作以简要的分析，以提高我们对冬虫夏草的进一步认识。

Hubei Shengfeng Medical Company has spent a lot of time and energy on the study of the Cordyceps sinensis and has produced a kind of similar herb. At first researchers collect funguses separated from Cordyceps sinensis that have the similar effective elements as Cordyceps sinensis. Then the funguses are inoculated on the larvae in the base. Much to the researchers' surprise, this kind of planted herb has even more nutrition and drug properties than the natural Cordyceps sinensis.

湖北圣峰药业有限公司从虫草菌天然型培养着手进行研究，从自然界采集冬虫夏草分离出来的真菌，药理功效与冬虫夏草的有效成份基本相似，接种在替代幼虫的富硒培养基上，进行固体静置培养菌丝体和分生孢子体作为有型冬虫夏草，经检测人工虫草的药性及营养成份不仅含量高於野生虫草，而且含有机硒。

Establish the method of HPLC fingerprints for analysing the Cordyceps sinensis. of differ origin or differ batch. The method of fingerprints was accurate and reliable. The high similarity between ten batch samples can futher verify reliability of quality assess studied by HPLC in Chapter 2.At the same time,it can also be applied to the quality control of Cordyceps sinensis .and also provide new reference for distinguishing counterfeits and adulterations.

本课题应用HPLC建立了不同产地不同批次冬虫夏草的指纹图谱测定方法，所建立的指纹图谱具有稳定重复的特点，10批冬虫夏草的高相似度支持了第二章中利用HPLC结论作为冬虫夏草内在质量评定参考的可行性，并可为冬虫夏草与其混淆品及伪品的质量控制提供新参考依据。

Objective: In this parper, we studied the key problems of resolution and determination,which haven,t been solved by HPLC in Cordyceps sinensis.,such as chromatography condition and extraction condition. Using the condition we studied out, we analysed the nucleosides in Cordyceps sinensis . and in its adulterations and counterfeits by HPLC. The purpose is to find the difference between Cordyceps sinensis . and its adulterations, and also to provide a effective method for quality assess of Cordyceps sinensis .

目的：本课题采用高效液相色谱法，通过对冬虫夏草中核苷类成分分离测定中的关键性问题进行考察，解决利用高效液相色谱分析冬虫夏草存在的问题，并利用该条件对冬虫夏草及其混淆品与伪品中核苷类成分进行分析研究，找出冬虫夏草与混淆品及其伪品在成分及含量上的差异，完善冬虫夏草质量评定标准。

HPLC chromatogram fingerprints were used to analyse ten batch Cordyceps sinensis. of different origin . The correlation coefficients between ten batches were use to define the similarity to fine the link with the result of HPLC, and also provide reference for distinguishing Compare Cordyceps sinensis. with its counterfeits and adulterations.

采用HPLC法对不同产地的10批冬虫夏草进行指纹图谱分析，建立共有模式，并以10批冬虫夏草的相关系数及相合系数评价指纹图谱的相似性，建立其与高效液相色谱研究结论的相关性，并为区分冬虫夏草及其混淆品和伪品提供参考依据。

HPLC method can determine the contents of the nucleosides and their bases in the Cordyceps sinensis. and in its adulterants and counterfeits. The results showed that the totle contents of uridine, hypoxanthosine, guanosine, adenosine, and the content of adenosine can be used as a effective method for quality assess of Cordyceps sinensis .and distinguishing its counterfeits and adulterations.

本课题建立了HPLC法检测冬虫夏草及其混淆品与伪品中核苷及核苷碱基含量的方法，可为冬虫夏草内在质量评定提供参考依据，并为冬虫夏草及其混淆品与伪品的区分提供一种判定依据，即可根据主成分I 、II、III、V四个核苷的总含量及V的含量作为区分冬虫夏草与混淆品及伪品的指标。

There were obvious difference in the characteristic peaks and the uncharacteristic peaks,when compared the adulterations and counterfeits with Cordyceps sinensis ..The relative peak aera and their fluctuate range of. the adulterations and counterfeits had obviously difference when compared to Cordyceps sinensis ..

冬虫夏草混淆品及伪品的高效液相色谱图与冬虫夏草指纹图谱比较，在共有峰以及非共有峰上都有较大差异，混淆品及伪品中各共有峰相对保留峰面积与冬虫夏草有明显差异，且混淆品及伪品相应共有峰的相对保留峰面积波动范围与冬虫夏草相比明显较大。

The result in this part accorded to that of HPLC in the second chapter(The totle content of uridine、hypoxanthosine、guanosine、adenosine in Cordyceps sinensis .was higher than 2.50mg/g, the content of adenosine was higher than 0.75 mg/g). The totle content of uridine、hypoxanthosine、guanosine、adenosine in the counterfeits and adulterations was from 1.219 mg/g to1.803mg/g ,the content of adenosine was from 0.364 mg/g to0.423 mg/g .

应用HPLC-MS对冬虫夏草及其混淆品与伪品进行二级质谱定量分析，结果显示，冬虫夏草中I 、II、III、V四个核苷的总量介于2.473 mg/g～2.830 mg/g，V的含量介于0.850 mg/g～1.009 mg/g，与第二章高效液相的研究结果吻合（冬虫夏草四个核苷成分的总量大于2.50mg/g，V的含量大于0.75 mg/g）。

HPLC/MS/ MS was used to analyse the nucleosides in Cordyceps sinensis .and its counterfeits and adulterations. Compared the samples with the standard samples,the result showed that the main fragment chromatogram were similar.The four nucleosides in Cordyceps sinensis .and its counterfeits and adulterations were identified by MS/ MS.The totle content of uridine、hypoxanthosine、guanosine、adenosine in Cordyceps sinensis .was from 2.473 mg/g to2.830 mg/g ,the content of adenosine was from 0.850 mg/g to1.009 mg/g .

应用HPLC-MS对冬虫夏草及其混淆品与伪品中的四个核苷成分进行二级质谱定性分析，并与对照品进行比较，结果显示，冬虫夏草及其混淆品与伪品均能产生与相应对照品一致的主要碎片离子峰，进一步说明，冬虫夏草及其混淆品与伪品中均含有尿苷、肌苷、鸟苷及腺苷四种核苷成分。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。