再配

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

The lighter-than-air melodies and samba-inflected dance beats, combined with the unflappable soprano of vocalist Nina Persson, deliver the punch of some weird amalgam of Blondie and Burt Bacharach.

轻柔似空气的旋律，配以桑巴舞曲的节拍，再加上NINA独一无二的轻柔嗓音，给观众带来了类似Blondie 和 Burt Bacharach组合的奇怪音乐效果。

It is indicated that the protective mechanism is related to the preservation of vascular endothelial cell function and intact basilemma, improvement of microcirculation and reduction of cerebral edema.

提示冰片与川芎配伍可通过保护血管内皮细胞功能、基膜的完整性，改善微循环，减轻脑水肿程度，从而对脑缺血再灌注损伤起保护作用。

Yesterday,the bunnies has been hybridization,and after a month the lovely baby rabbit will seen--little coved with white hair use their mouth chewing.

昨天，伊拉兔配了种，再过一个月也一定能看到我想象中的那种可爱的兔宝宝的吧－－小小的、全身长了白毛的家伙会用它的三瓣嘴快速地咀嚼。

This study has introduced the relationship matrix between Kano's two-dimensional quality elements and quality improvement modes and importance of quality elements by double confirmed. According to judgement on decrement dissatisfaction more than increment satisfaction, the 3×3 matrix is composed of Kano's two-dimensional quality elements and quality improvement modes, that have 9 sets compose,it is supplyed 1~9 score or weight dividedly.

本研究推导出Kano二维品质属性与品质改善模式之对应关系，并以Kano二维品质模式归类结果及品质改善模式图对品质属性之重要度做双重确认；再依消除不满意优先於增加满意实施之判断，对「二维品质归类-改善模式」组成之3×3矩阵，分别给予9种组合1~9分不等的配分或权重。

The epineurial vessels run along the nerves length and gave off branches, distributing along nerve bundle, to enter into endoneurium. The endoneurium contained only capillaries. Arteriolovenular anastomoses were few in trigeminal nerve root. The distribution of microvessels in trigeminal nerve root was uneven. The number of microvessels in the medial portion was less than that of the distant.

神经外膜血管沿神经根径向走行，沿途再发侧支斜行或横行入神经束之间，顺神经根束分布，并发侧支进入神经内膜；半月结内微血管围绕神经节细胞分布；三叉神经根内的微血管配布不均，其近侧端微血管数量较少，远侧端微血管数量较多。

Firstly,two novel rare earth complexes(CH_2=CHO)Ln~Ⅲ[2,6-_2PhNSiMe _3]_2Li_3Ln=Y(2,Yb(3)containing arylamido and enolate mixed ligands have been synthesized by treatment of 2 equiv of of 2,6-~iPr_2PhNHSiMe_3 with exces n-BuLi at -78℃in THF for 12h,followed by reaction with 1 equiv of LnCl_3at 80℃for 24h in one-pot.All the two compounds were characterized by spectroscopic methods,elemental analyses,and a single-crystal X-ray diffraction study.

首先，-78℃下，在两当量的2，6-~iPr_2PhNHSiMe_3(1)THF溶液中加入过量的n-BuLi，反应12小时，再加入一当量的LnCl_380℃下，反应24小时，一锅法合成了含胺基、乙烯醇基混合配体的新型稀土金属配合物(CH_2=CHO)Ln~Ⅲ[2，6-_2PhNSiMe_3]_2Li_3Ln=Y(2、Yb(3)，上述化合物结构经单晶X-ray衍射、光谱和元素分析表征，表明我们得到的是一类新型的三价稀土配合物。

Notes to attention: when adjust colorful transparent groove, test is needed to determine use it or not use it as obvious shrinkage may encountered at spiculate margin caused by the fuscous.

注意事项：在配彩色透明槽时，要特别注意本漆对很浓很深的颜色，在尖锐的边角处可能会有明显的缩边现象，需要事先加以实验后再确定使用或不使用。

Gyratory machine was used to design Superpave mixture, Marshall sample were also prepared with the same gradation and asphalt content.

采用Superpave方法成型旋转压实试件，进行混合料设计，确定混合料级配和沥青用量，再按照确定的级配制作马歇尔试件，获取2种方法的体积指标以进行对比。

Different from the conventional catalyst immobilization methods, the self-supporting strategy utilizes homochiral metal-organic coordination polymers generated by self-assembly of chiral multitopic ligands with metal ions without using any support.

与传统的负载模式不同，手性自负载催化剂通过含双或多官能团的手性配体与金属通过自组装形成一类有机-无机聚合物，因此无需使用任何载体，即能够有效地实现手性催化剂的回收和再利用。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。