再连接

At aim of getting the full 235 bp gene, five 50~70 bp model-based primers were first synthesized by chemical method followed by 3-step PCR amplification in an effort to ligate them into full gene.

为了得到目的肽全基因，首先合成5条50～70 bp互为模板引物的DNA片段，再通过PCR扩增将5条片段连接成全基因。

Methods Activate groups A and C meningococcal polysaccharide with cyanogens bromide and prepare into MenAPS-ADH and MenCPS-ADH derivatives by reacting with 1, 6-adipic acid dihydrazine. Mix MenAPS-ADH and MenCPS-ADH derivatives with purified tetanus toxoid at a certain mass ratio, then add l-ethyl-3(3-dimethylaminopropyl)-carbodiimide to prepare bulks of MenAPS-TT and MenCPS-TT conjugates respectively. Mix the two bulks at a certain ratio, add stabilizer and lyophilize to prepare group A+C meningococcal polysaccharide conjugate vaccine.

A群和C群脑膜炎球菌多糖在溴化氰的活化下，以1，6己二酰肼作为连接子，与精制破伤风类毒素在碳二亚胺作用下结合，再按一定的比例将两者混合，制成A+C群脑膜炎球菌多糖结合疫苗原液，加保护剂冻干后，制成A+C群脑膜炎球菌多糖结合疫苗。

Dissect the fascia from the dorsum down to the bursa over the medial aspect of the metatarsal head. Then make a curved incision through the bursa and capsule of the joint Fig. 1-24,; begin the incision over the dorsomedial aspect of the joint, continue it proximally dorsal to the metatarsal head and then plantarward and distalward around the joint, and end it distally on the medioplantar aspect of the metatarsophalangeal joint.

然后弧形切开滑囊及跖趾关节囊图1-24，，切口起自关节背内侧，自跖骨头背侧向近端延长，然后转向跖侧，再转向远瑞，环绕关节而行，最后在远端终止于跖趾关节的跖内侧面，此切口形成一椭圆形网球拍状组织瓣，连接于近节趾骨的基底部

While AcMNPV and BmNPV had no stimulatory effect in nonsensitive cell lines BmNPV hr3 has been demonstrated to increase the transcriptional activity of cislinked hel510 promoter. In this study, Sf-21, Bm-N and Hi-5 cell lines were transfected with pBmhel510luc-hr3down, and then were infected with each of AcMNPV, BmNPV and HyNPV. The results indicated that viral factors could interact with hr3 to significantly copromote the transcriptional level of hel510 promoter.

BmNPV hr3已被证明具有增强子功能，将hr3与hel510启动子顺式连接的质粒pBmhel510luc-hr3down转染Sf-21、Bm-N和Hi-5细胞后，再用上述三种病毒分别感染每一种细胞，hel510启动子的转录活性得到显著提高，表明病毒因子能够识别hr3，二者通过相互作用，共同增强解旋酶基因启动子的转录水平。

In this study, the structure sequences of Shiga toxin 2B(stx2B) and Shiga toxin 1B(stx1B) were respectively amplified from EHEC O157:H7 by PCR. stx2B-L-stx1B fusion gene was constructed by a 12 bp linker ligating these two fragments. After digested with restriction endonuclease Nco Ⅰ and EcoR Ⅰ, the fusion gene was orientally inserted into expression vector pET28a.

利用PCR方法，从肠出血性大肠杆菌O157：H7的基因组DNA中，分别扩增出Stx1B和Stx2B亚基的结构基因片段，然后再通过PCR将这2个片段连接起来，并定向克隆到表达质粒pET28a的NcoⅠ和EcoRⅠ位点之间，构建了重组表达质粒pMB1。

The first step in the degradation is most probably the classical ester scission reaction to give a carboxylic acid and olefinic end groups. Further ester scission on the adjacent ester groups of carboxylic acid and olefinic molecular chains can then lead to the formation of lower weight pyrolytic products, such as: CH〓=CHOCOPhCOOCH=CH〓、CH〓=CHOCOPhCOOH and HOOCPhCOOH, and they can pyrolyze continually to produce substrate pyrolytic compounds, such as: HOOCPhCOCH〓、PhCOOH、PhH、PhCOCH〓 and CO、CO〓. At the same time, a corsslinking cyclization reaction occurred of the formation of the gas products during the degradation of PET.

断裂大分子链端邻近羰基和乙烯基的酯键持续断裂，不断地形成小分子裂解产物，如：CH〓=CHOCOPhCOOCH=CH〓、CH〓=CHOCOPhCOOH以及HOOCPhCOOH，这些产物还会进一步裂解成分子量更低的小分子裂解产物，如：HOOCPhCOCH〓、PhCOOH、PhH、PhCOCH〓以及CO、CO〓等，与此同时，一部分乙烯基酯端基的大分子链碎片会发生支化而相互连接，再经&链脱离&和环化反应形成难燃的交联产物。

When you connect to an ISA Server 2004 computer, the ISA Server computer may not maintain your credentials, and you may have to reauthenticate the next time that your client makes a request.

当你连接到一个 ISA 伺候器 2004部计算机的时候，ISA 伺候器计算机不可能维持你的国书，而且你可能下回必须再证明你的客户要求。

If you have a script, you just have to issue the query again for the client to do an automatic reconnection.

如果你使用脚本，你只能在客户端重新连接再查询。

For manufacturing a variety of tower internals. It especially produces different kinds and types of tower in ternals, including all kinds of trays and their installation hardware, various random packings and structured packings, liquid and gas distributor, liquid collector and redistributor, and so on.

专业生产各种型号、规格的塔内件产品，包括各种塔板及其连接紧固件、各种散装填料和规整填料、液体及气体分布器、液体收集器和再分布器等。

The user snaps a photo, highlights a building or mountain or some other major site, then waits as the object-recognition program, developed by SuperWise Technologies AG, identifies this image and links it to the relevant facts.

用户摄到一张照片后，着眼于某一座建筑物或某一座山脉或某个其它主要场所，再等待由高新技术公司研制的目标识别程序鉴定该图像且将它连接到相关数据。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新