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Results (1)ATP could inhibit the proliferation of U937 cells with inhibition rate over 43% after 48hour ATP treatment;(2)ATP treated U937 cells numbers increased obviously in G1 phase,and apoptosis peak appeared before G1 phase,apoptotic cell number was 3.4% for 24hour,and was 22.7% for 48hour of ATP treated U937 cells;(3) Nuclear chromatin condensed into sperical masses bound cytoplasma membrane formed apoptotic bodies which is shed from membrane surface into intercellular medium;(4) Apoptotic bodies were nigrosine staining negtive.

结果 (1)ATP对U937细胞的增殖有按摩明显的阻抑作用,加药48h后增殖的抑制率可达43%以上;(2)ATP处理的U937细胞周期发生改变,G1期细胞数按摩明显增多,ATP作用24h时G1期前出现亚二倍体峰——凋亡峰,凋亡细胞数为3.4%,作用48h时凋亡细胞数增多为22.7%;(3)ATP处理的U937细胞首先在核内染色质浓缩成半月形贴近核膜,逐渐向核膜外移动,进入胞浆内再移向质膜内外面,紧贴质膜外面再逐步脱离细胞体,进入细胞基质中,成为游离的凋亡小体。

objective to evaluate efficacy of extracorporeal shock wave lithotripsyfor treating ureteral stones in situ,investigate the cause of higher re-treatment rate.methods total of 687 patients with ureteral stone were received eswl between january 2000 and december 2004,included 455 male(66.2%) and 232 female(33.8%) patients,6 cases have bilateral ureteral calculi,12 cases have unilateral multiple calculi.hence,together 709 ureteral calculi were treated.patients upper ureteral calculi were treated in the supine position,for lower ureteral calculi patients were turned prone.to reduce eswl-induced renal trauma and pain,using lower energy source,adjusted power setting from 9.8 to 13.2kv,limited 1500 shock wavs per one session.no auxiliary procedure were used before eswl.the stone size was measured as the surface area of stone length by stone width on x-ray film.the interval between two treatment sessions was two weeks.results of 709 ureteral calculi,the overall stone free rate was 97.3%(690 calculi),re-treatment rate was 34.1%(292 calculi).according to the performed treatment sessions,one session 467 calculi,the mean stone size 37.27mm2,stone free rate 65.4%(464 calculi).two sessions 138 calculi,the mean stone size 62.48mm2,stone free rate 18.4%(131calculi).three sessions 52 calculi,the mean stone size 79.60mm2,stone free rate 7.1%(50calculi).four sessions 19 calculi,the mean stone size 101.63mm2,stone free rate 2.4%(17calculi).fivesessions 33 calculi,the mean stone size 119.33mm2,stone free rate 3.9%(28 calculi).overall 19 cases(2.7%)turned to other treatment modalities.of 335 upper ureteral calculi,303 achieved stone free (95.8%),re-treatment rate was 38.5%(129 calculi).of 374 lower ureteral calculi,369 achieved stone free(98.7%),re-treatment rate was 30.2%(113 calculi).the re-treatment rate of upper ureteral calculi was higher than lower ureteral calculi(p<0.05,χ2=5.40).the difference of stone-free rate between upper and lower ureteral calculi was no significant(p>0.05,χ2=0.15).conclusion eswl should be considered first line therapy for ureteral stone still.stone burden are the main variable of higher re-treatment rate,upper ureteral stone may moving with respiring during eswl.so efficinet shock wave was decreared,re-treatment rate become higher.

目的 评估体外震波碎石治疗输尿管结石的疗效,探讨再治疗率高的原因及输尿管结石的治疗选择。方法回顾2000年1月~2004年12月间eswl治疗输尿管结石的临床资料687例,男455例(66.2%),女232例(33.8%),平均年龄46.6岁(15~83岁)。有双侧输尿管结石6例,单侧多发性输尿管结石12例(4颗1例,3颗2例,2颗9例),共计输尿管结石709颗(含透光结石13颗)。应用上海爱申公司生产的desunit6030型碎石机,c臂x线球管做结石定位。上段输尿管结石(肾盂输尿管交界处至骶髂关节上缘)取仰卧位,下段输尿管结石(骶髂关节上缘下至输尿管口)取俯卧位。为减少eswl引起的肾损伤和疼痛,应用较低的能量,震波发生器电压从9.8~13.2kv,震波频率1.5s。每次治疗设定为1500次震波。治疗后3天摄腹部平片或b超,以后每隔7日重复检查。假如结石未碎或有残留结石最长径>3mm以上,再次eswl,两次治疗的间隔时间为两周。结石的大小用x线片上的表面积(mm2表示。结果 709颗输尿管结石总的治愈率为97.3%(690颗),再治疗率34.1%(242颗)。其中一次治疗467颗,平均结石大小37.27mm2,治愈464颗(65.4%),3颗改治疗;两次治疗138颗,平均结石大小62.48mm2,治愈131颗(18.5%),7颗改治疗;第1和第2次治疗治愈率(1个月治愈率)为83.8%。3次治疗52颗,平均结石大小79.60mm2,治愈50颗(7.1%),2颗改治疗;4次治疗19颗,平均结石大小101.63mm2,治愈17颗(2.4%),2颗改治疗;5次及5次以上治疗33颗,平均结石大小119.33mm2,治愈28颗(3.9%),5颗改治疗。总计19颗(2.7%)结石改变治疗方式。上段输尿管结石335颗,治愈321颗(95.8%),再治疗129颗(38.5%)。下段输尿管结石374颗,治愈369颗(98.7%),再治疗113颗(30.2%)。经χ2检验,上、下段输尿管结石的再治疗率差异有显著性(χ2=5.40,p<0.05),治愈率差异无显著性(χ2=0.15,p>0.05)。不良反应:血压升高13例(1.9%),震波区域疼痛26例(3.8%),震波进入处皮肤点状淤血33例(4.8%),肉眼血尿128例(18.6%),均于第2、3天自行消失。结论 eswl目前仍是输尿管结石的第一线治疗,结石的大小是再治疗率高的主要因素。结石的位置有影响,上段输尿管结石可随呼吸移动,有效震波次数减少,再治疗率比下段输尿管结石高。eswl前注重病例筛选可降低再治疗率。

"The next step on the agenda is to test the method on actual grapevines and then to carry out field tests."

&下一步议程,是对真正的葡萄树进行测试,然后再进入田间试验。&

You won't be able to re-use the same pool numbers from week to week without re-entering them into the program.

您将无法重新使用同一批号码,从周,以周没有再进入他们纳入计划。3。

That started a global roadshow which reached Europe by the end of the week, and will then move on to America.

启动全球巡回达到了欧洲,到本周末,然后再进入美国。

Forming mechanisms of the spin glass state and re-entrance phenomenon of B2 type FeAl compound were analyzed, and it was thought that different mechanisms existed in FeAl compounds with various ranges of compositions such as indirect exchange interaction, short-range ordering of partial atoms, and finite and infinite radical coexistence.

研究了不同成分的B2型FeAl化合物磁化强度与外场的关系、磁化强度与温度的关系、磁滞回线的特点,分析了B2型FeAl化合物自旋玻璃态、再进入现象的形成机制,认为FeAl化合物不同成分范围内应存在不同机制如间接交换作用、部分原子短程有序、有限、无限原子集团作用并存。

All the guests should register at the reception desk before entering the hall.

所有访客应该在接待台登记后再进入大厅。

Chemical treatment is carried out periodically, after a course of chemotherapy after a period of time to rest before the next treatment cycle.

化学治疗是周期性地进行,经过一次化学治疗疗程後会休息一段时间,然後再进入下一个治疗周期。

Discovery landed after safely crossing the continent in the first coast-to-coast reentry since the Columbia disaster.

在继哥伦比亚的灾难后,&发现号&第一次安全地从大西洋岸到太平洋岸的再进入并穿越了大陆后降落。

There is no direct conclusive evidence that the mechanism of cell cycle may involve apoptosis.

此外,目前也没有证据表明细胞凋亡和细胞周期再进入之间的因果关系。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

聚光:照片上是建在西班牙桑路卡拉马尤城的一座新型PS20塔式太阳能电站。被称为&日光反射装置&的镜子将太阳光反射到主塔,然后用聚集的热量产生蒸汽进而通过涡轮机转化为电力