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The total RNA from brain tissue of substantia nigra of PD rats is extracted at the 1st, 2nd, 4th and 8th week and comparison is made between it and that from normal rats. The total RNA, after RNA formaldehyde degenerated sepharose electrophoresis and separation and Northern transfer, is adsorbed unto colloxylin membrane. Then ECL labeled Selenoprotein P and an unknown gene fragment are used as probes to hybridize with the RNA on the colloxylin membrane. And the hybridized result is obtained with enzyme-linked immunosorbent assay. Finally, analysis of OD is made using ONE-Dscan software.

分别提取1周、2周、1月、2月的帕金森大鼠(各8只)黑质区脑组织总RNA,并取相对应时间的正常大鼠的脑组织总RNA作为对照,经RNA甲醛变性琼脂糖凝胶电泳分离后,通过Northern转移,将其吸附在硝酸纤维素膜上,然后利用ECL标记的Selenoprotein P和一段未知基因为探针,与硝酸纤维素膜上的RNA分子进行杂交,再通过显影取得杂交的结果,应用ONE-Dscan软件进行光密度值分析。

The influences of the concentration and generation of polyamidoamine dendrimer, the ratio of dodecanoic acid to toluene, and pH in the water phase on the extraction yield of helianthine were systematically studied by selecting helianthine as the guest molecule and electrostatic self-assembled PAMAM as the host.

本文采用低支化树枝状大分子聚酰胺胺与月桂酸进行分子自组装得到了一系列具有稠密的疏水性外围和亲水性内核的自组装大分子,再以自组装大分子为宿主,系统地研究了树枝状大分子聚酰胺胺的浓度、支化代、月桂酸与甲苯的质量比及水相pH值对客体分子甲基橙相转移行为的影响规律。

During angiographing,3-link chemotherapy medicine was administered through the artery to carcinoma and embolization with proper amount of iodinate oil and gelfoam according to tumor size and posit...

在血管造影的同时,根据肿瘤大小、部位以及肾门淋巴结转移情况,经供瘤动脉给予三联化疗药物,再用适量碘化油乳剂及明胶海绵条给予栓塞。

Method]from january 2003 to may 2006,32 patients were corrected with qin si-he's orthotics devices on the ilizarov principle of tension-stress,which involved 15 males and 17 females,the age ranged from 10 to 25 years.among these patients,2 were caused by peroneal nerve injury,l by tumor in the vertebral canal,5 by meningocele,11 were caused by poliomyelitis,13 by congenital talipes equino-varus.in accordance with deformities,external fixator and limitied operative methods were dertermined.the limited release of soft tissue were performed in 7 patients,limited osteotomy in 25 patients.the dynamic muscle balance operation were performed in 9 patients with imbalance of muscle strength.according to the ilizarov technique,the fixative rods were installed.the telescopic rods on the apparatus were rotated one week after the operation,the divices had corrective function in three-dimensional directions.the deformity of talipes equinovarus,internal rotation and drooping of the forefoot were gradually corrected,and the patients could bear weight and walked on the deformed foot.the mean duration of traction were 42 days,then removed the external fixator maintained with plaster for a site time.

方法]2003年1月~2006年5月,根据ilizarov张力应力法则,应用秦泗河改良的外固定矫形器,遵循ilizarov穿针固定的基本原则,共手术治疗马蹄内翻足32例,男15例,女17例;年龄10~25岁,平均17岁。病因:腓总神经损伤2例,腰椎管内肿瘤1例,硬脊膜膨出5例,小儿麻痹后遗症11例,先天性马蹄内翻足13例。术前用足掌的前外缘负重行走者11例,用足的外缘或足背外侧负重者21例。根据马蹄内翻足畸形程度、性质和患者年龄,确定实施有限矫形手术的方法和外固定矫形器治疗。本组7例同期实施有限的软组织松解术,25例同期实施了有限的截骨术和跗骨间关节融合术,9例合并踝关节内外翻肌力明显失衡者,同期行足部肌腱转移的肌力平衡术。然后安装外固定矫形器。术后按作者制定的管理程序,7 d开始旋转相应的螺纹牵拉杆,对器械进行三维空间的缓慢调整,先矫正前足内收和后足内翻,后矫正足下垂畸形,直至达到矫形要求的标准。在矫形的过程中定期进行x线检测,以防止发生踝关节前后移位,治疗期间允许患足负重行走。术后平均牵伸42 d,拆外固定器后患足再上石膏固定适当时间。

Etymologically, the word'metaphor'originates from the Greek word'metaphora', which consists of two roots:'meta'and'phora', indicating the expression of the entailments of one meaning in terms of another.

英语里'metapho'一词源于两个希腊语词根'meta'和'phora'组成的'metaphora',指的是将某种事物的内涵转移到另一种事物上再表达出来。

This shift in spending from the West to the East will help rebalance the world economy.

这种消费从西向东的转移对世界经济的再平衡有所裨益。

What what differ is, the article undertook redivide to the economic area of our country and economic gradient, if why manufacturing industry of coastal to southeast treatment undertakes between domestic area gradient move undertook new reflection.

所不同的是,本文对我国的经济区域和经济梯度进行了再划分,并对东南沿海加工制造业如何在国内区域间进行梯度转移进行了新的思考。

Recently, Shelnutt and co-workers obtained discrete porphyrin nanosheets reprecipitated from their solutions;[3] Sathish and co-workers constructed hexagonal C60 nanosheets using a liquid–liquid interfacial precipitation method;[4] the groups of Yao[5] and Hu[6] prepared singlecrystalline nanosheets of polycyclic aromatics using a surfactant- assisted reprecipitation and a physical vapor transporting method, respectively; and Zhang and co-workers suggested that molecules with intramolecular charge-transfer dipole moments could be grown into quasi-2D nanostructures.

最近,舍纳特和同事获得离散卟啉薄片reprecipitated其解决方案; [3] Sathish和同事构建六角 C60的薄片采用液液界面沉淀方法; [4]姚明集团[5]和胡[6]准备singlecrystalline 多环芳香烃薄片的使用表面活性剂再沉淀和辅助物理气相传输法分别,张和同事建议随着分子内电荷转移分子偶极时刻可以发展成为准二维纳米结构。

Recently, Shelnutt and co-workers obtaineddiscrete porphyrin nanosheets reprecipitated from theirsolutions;[3] Sathish and co-workers constructed hexagonalC60 nanosheets using a liquid–liquid interfacial precipitationmethod;[4] the groups of Yao[5] and Hu[6] prepared singlecrystallinenanosheets of polycyclic aromatics using a surfactant-assisted reprecipitation and a physical vapor transportingmethod, respectively; and Zhang and co-workers suggestedthat molecules with intramolecular charge-transfer dipolemoments could be grown into quasi-2D nanostructures.

近日,舍纳特和同事获得离散卟啉薄片reprecipitated其处理方案; [3] Sathish和同事构建六角C60的薄片采用液液界面沉淀方法; [4]姚明集团[5]和胡[6]准备singlecrystalline多环芳香烃薄片的使用表面活性剂再沉淀和辅助物理气相传输法分别,张和同事提议随着分子内电荷转移分子我极时刻可以发展成为准二维纳米结构。

Attempt to toprope the entire climb with a sustained, narrow focus fixed on your feet. With each placement, look for the best part of the hold and actually watch your foot go onto the hold in an ideal placement.(You may be surprised to know that many climbers don't see their feet go on the holds!) Maintain your focus on that foot as you weight the hold--strive to feel each foot hold and to keep your foot completely still as you stand up on the hold. Meanwhile, place as little focus as possible in other areas such as reading the route ahead, your belayer, and so forth.

尝试顶绳持久的攀爬整条线路,注意力全部集中到你的脚上,在每个移动中,寻找脚点的最好的位置,然后看着你的脚踩上理想的位置(你可能会很惊奇的发现很多攀登者都没有看着自己的脚踩上脚点),当你将重心逐渐转移到这个点上时,保持你对脚的关注,努力去感受每个脚点,当你站在点上时,脚不要再移动,尽量少去注意其他的区域,例如提前观察线路、你的保护者等等。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口,替代木托盘,免薰蒸,符合欧盟、北美各国对出口货物包装材料的法令要求;喷涂钢托盘适用于重载上货架之用,托盘表面根据需要制作成平板状、波纹状及间隔形式,满足不同的使用要求。

A single payment file can be uploaded from an ERP system to effect all pan-China RMB payments and overseas payments in all currencies.

付款指令文件可从您的 ERP 系统上传到我们的电子银行系统来只是国内及对海外各种币种付款。