再转移

Attempt to toprope the entire climb with a sustained, narrow focus fixed on your feet. With each placement, look for the best part of the hold and actually watch your foot go onto the hold in an ideal placement.(You may be surprised to know that many climbers don't see their feet go on the holds!) Maintain your focus on that foot as you weight the hold--strive to feel each foot hold and to keep your foot completely still as you stand up on the hold. Meanwhile, place as little focus as possible in other areas such as reading the route ahead, your belayer, and so forth.

尝试顶绳持久的攀爬整条线路，注意力全部集中到你的脚上，在每个移动中，寻找脚点的最好的位置，然后看着你的脚踩上理想的位置（你可能会很惊奇的发现很多攀登者都没有看着自己的脚踩上脚点），当你将重心逐渐转移到这个点上时，保持你对脚的关注，努力去感受每个脚点，当你站在点上时，脚不要再移动，尽量少去注意非法信息已被屏蔽的区域，例如提前观察线路、你的保护者等等。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

Tool targeting process in this method only requires the image-to-patient registration, in which an automated laser surface scanner is used to unitize the spaces of patient and robot.

本方法的特性是利用自动雷射曲面扫描系统扫描病患脸部特徵及手术器械位置，之后再和医疗影像进行特徵点及曲面最佳化叠合，可直接将医疗影像的空间座标转移到机械手臂的座标架构。

It means the simultaneous problem regarding the labour amount and the employment structure; causes the circumstance that the increasing employing pressure in urban areas emerges at the same time as the rural extra labours accelerates to move to unrural areas; results in the mutual problem with the newborn labours' employment and the out of work's reemployment.

主要表现在：劳动力供求总量矛盾和就业结构性矛盾同时并存，城镇就业压力加大和农村富余劳动力向非农领域转移速度加快同时出现，新成长劳动力就业和失业人员再就业问题相互交织。

Mainly present: the total amount of labour surporting and the the contradiction of the employment structure both exist,which causes the circumstance that the increasing employing pressure in urban areas emerges at the same time as the rural extra labours accelerates to move to unrural areas; results in the mutual problem with the newborn labours' employment and the out of work's reemployment.

主要表现在：劳动力供求总量矛盾和就业结构性矛盾同时并存，城镇就业压力加大和农村富余劳动力向非农领域转移速度加快同时出现，新成长劳动力就业和失业人员再就业问题相互交织。

Blindside 攻其不备 You are able to divert your enemy's attention with a single melee strike and follow it with a single bow-attack at close range.

你可以采用一个近战攻击转移敌人的注意，然后再近距离用弓箭攻击。7 T( l2 s1 W) h7 G9 Q F

For collection pay to the order of B Bank Pay to the order of B Bank for collection only,prior indorsement guaranteed Pay to the order of B Bank of deposit Pay to the order of B Bank value in collection Pay to the order of B Bank by procuration Pay to any Bank 69 (5)质押背书 pledged

质押背书是背书人以票据的债权作抵押物而对票据所作的一种背书。质押背书并不是债权的转移如果被背书人进行再背书，只能以委托收款的方式。

In this article, the factors that affect molecular weight and solubility of polyacrylamide are studied and suitable initiator system is selected. The optimum parameters of copolymerizing process are obtained as follows: the molar proportion of acrylamide and sodium acrylate is 7:3, the monomer concentration is 30%, pH value of solution is 12, the oxidizer (K2S2O8) concentration is 0.1%, the reducing agent (NH2CONH2) concentration is 1%, the chain-transfer agent concentration is 0.4%. The initial reaction temperature is 35℃.

讨论了影响聚丙烯酰胺分子量和溶解性能的因素，选取了合适的氧化-还原引发体系，确定了制备超高分子量速溶型部分水解聚丙烯酰胺的优化聚合工艺参数：起始共聚合反应温度为35℃，保持在密闭条件下的共聚合反应2.5h后，再将聚合反应温度升高到55℃，继续反应2h，反应结束；反应溶液单体总浓度：30％，共聚合反应溶液pH值：12，氧化剂K_2S_2O_8：0.1％，还原剂NH_2CONH_2：1％，共聚合反应中两种单体的投料比：丙烯酰胺：丙烯酸钠=7：3，异丙醇链转移剂：0.4％。

Make "moving paths" be a process of experiencing a space. By empathizing and understanding in the process, make it become a picture book, then make the story in the picture book into the tension of real spaces.

将「移动中的路径」作为体验一个空间经验的过程，藉由人在体验中的移情与理解之后，架构成绘本故事，然后再将此故事性转移成具体空间的张力。

Transfer the dough to a lightly floured work surface, knead the dough a few times to bring it together, and then divide the dough in half.

转移到面团轻轻面粉的工作表面上，揉面团几次使其在一起，再除以面团一半。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。