再起

Through a variety of resin filter, adsorption and elution conditions for the optimization, as well as decolorization, crystallization methods of research, this article eventually determine the strong acid cation resin HZ-001 which is at pH 3, 3BV / h, having the largest adsorption to the tryptophan; the fermentation broth were centrifuged to remove sediment,then treated with activated carbon to be decolored and then treated with strong acid cation resin HZ-001. After that,2mol/l amino acid were passed through the HZ-001 ion exchanging column,kept almost constants when the flow rate stayed 6 BV / h. At 60℃,it was condensed into the saturated state and then added Ethanol until its percentage reaching 30%. At 4 ℃ under 100rpm placed in the shaker 20h, the collection of L-tryptophan was dried up in vacuo.

本文通过对种树脂的筛选、吸附和洗脱条件的优化，以及对脱色、结晶方法的研究与摸索，最终确定强酸型阳离子树脂HZ-001在pH=3，3倍床体积流速，温度室温，对色氨酸有最大的吸附作用；将发酵液离心去沉淀并用活性炭脱色后通过HZ-001强酸型阳离子交换树脂柱，再用2mol/L的氨水6倍床体积流速在室温下进行洗脱，收集洗脱液，60℃浓缩为过饱和状态后加入30%无水乙醇，4℃下100rpm摇起晶20h，过滤搜集结晶并真空干燥得白色粉片状L-色氨酸晶体。

The Russian army was defecting, they were picking up their toys and going home, they didn't want to play war anymore, they didn't like the Czar.

俄军也在叛变，他们捡起他们的玩具回家了，他们不想再打仗，他们不喜欢沙皇。

It also points out the facilitation of high-tech to sustainable development in the knowledge economy era and finally deliberates on the measures for the realization of S.

首先阐述了高技术的特征，再识了可持续发展的涵义，接着对实现可持续发展所面临的危机进行详细分析，指出高技术在知识经济时代对可持续发展所起的促进作用，最后着重阐述了高技术促进可持续发展实现的对

Even if erects again immediately, feminine bioelectricity also because penis degree of hardness will reduce, will reduce.

即使马上再勃起，女性生物电也会因为阴茎硬度降低，降低。

None of these little flurries , but big , fat flakes which clung immediately to their surface.

当我再起步时，无意间回想起刚才所经历的一切，镇定、释然。

As in my present Condition there were not really many Things which I wanted; so indeed I thought that the Frights I had been in about these Savage Wretches, and the Concern I had been in for my own Preservation, had taken off the Edge of my Invention for my own Conveniences; and I had dropp'd a good Design, which I had once bent my Thoughts too much upon; and that was, to try if I could not make some of my Barley into Malt, and then try to brew my self some Beer: This was really a whimsical Thought, and I reprov'd my self often for the Simplicity of it; for I presently saw there would be the want of several Things necessary to the making my Beer, that it would be impossible for me to supply; as First, Casks to preserve it in, which was a Thing, that as I have observ'd already, I cou'd never compass; no, though I spent not many Days, but Weeks, nay, Months in attempting it, but to no purpose.

就我目前的境况而言，我其实不缺多少东西。可是，我总感到，由于受到那些野蛮的食人生番的惊吓，因而时时为自己的安全而担惊受怕。以往，为使自己的生活过得舒服，我充分发挥了创造发明的才能，但现在就无法充分发挥了。我本来有一个煞费苦心的计划，想试验一下能否把大麦制成麦芽，再用麦芽来酿起酒。现在，这一计划也放弃了。当然，这实在也是一个荒唐的念头，连我自己也经常责备自己把事情想得太简单了。因为我不久就看出，许多酿造啤酒必不可少的材料我都没有，也无法自己制造。首先，没有啤酒桶。前面说过，我曾尝试做木桶，但怎么也做不好。

From printing to burning or fumigating, these elements may be seen as modeling with aesthetic ends, but from the beginning of paper, each modeling, including printing and further physical formation, is in fact a damage to the intact paper by the author, or rather the power represented by the author.

及至印刷，再到烫烧或烟熏。这几个环节，都可以被认为是塑造，而结果也是符合审美的。但是从纸张的诞生起，每一次的塑造，包括印刷和下一步的物理改造，都是将作者，或者说是以作者为代表的强势对原本完整的纸张的破坏。

They said your smiling face, also attractive down and out; In my life warm that many, I have given you completely, but will you leave me, you have called me later how again to smile to others; Once also some smiling face appeared in mine life, but finally like fog dissipation, but that smiling face, became rapids rivers which in my heart deep buried deeply, was unable to swim across, that rivers sound, became singing which my each night despaired every day; Every world making noise with bright, the common custom joy and the unhappiness, is similar to the clear mountain stream, in the wind, in me at present, gurgles, is similar to the water seepage to well up equally warm, I do not have the wild hope, so long as I you are joyful, do not have to be sad; The wind blows like the flower stave fleeting time, but your smiling face sways, becomes in my life the most beautiful embellishment, looks the day, looks at the snow, looks at the season deep shadow; A person must walk the strange road, looked the strange scenery, listens to the strange song.

他们说你的笑容，又漂亮又落拓；我生命里的温暖就那么多，我全部给了你，但是你离开了我，你叫我以后怎么再对别人笑；曾经也有一个笑容出现在我的生命里，可是最后还是如雾般消散，而那个笑容，就成为我心中深深埋藏的一条湍急河流，无法泅渡，那河流的声音，就成为我每日每夜绝望的歌唱；凡世的喧嚣和明亮，世俗的快乐和幸福，如同清亮的溪涧，在风里，在我眼前，汨汨而过，温暖如同泉水一样涌出来，我没有奢望，我只要你快乐，不要哀伤；风吹起如花般破碎的流年，而你的笑容摇晃摇晃，成为我命途中最美的点缀，看天，看雪，看季节深深的暗影；一个人总要走陌生的路，看陌生的风景，听陌生的歌。

Now come on, and I hauled him up once more.

好，再来，我又一次将他拉起。

When you pick it up then let go, it hovers at exactly the right height to mount

如果你拿起它再放开，它就会悬空在正好能骑上去的高度

By the time of its fall, most of the prisoners were writers who had written against the corruptions of the government.

到它被攻陷的时候，里面多数的犯人是写了反对政府贪污文章的作家。

The most obvious variation to ovum morphological character was that the color was changed from light green to sepiaceous in embryonic development, and all the ovums were almost hatched after 96h.

在胚胎发育过程中卵的形态特征最明显的变化是颜色从淡绿到深褐色，卵在发育96h后卵基本全部孵化。