再读

Applicants who have obtained approval in-principle may enroll in other reimbursable course and need not submit a second application.

申请人如已获得初步批准，可报读其他&可获发还款项课程&而不需再递交第二份申请。

Recently, a girl wrote this letter to me:Dear R.L. Stine, I really enjoy readingyour Goosebumps books, but I bet you can make them much shorter. I think youcould get them down to 25 pages-- if you really tried!

亲爱的斯坦先生，我非常喜欢读鸡皮疙瘩，但是我保证你和一定可以把故事写得再短一点，如果你很累的话，我想你可以把他们缩减到25页。

After that our principal stayed outside until the end of the reading time,he started to blame us stormily while he had come back to the class.Although the guitar is back in Helex's hands,it would be broken by our principal if this situation happens again.

之后校长在班外等到晨读时间完后，就返回班房，大闹一番，吉他虽然回到了希力的手中，但是这情况若再发生，恐怕会真的给校长摔破。

One thing I've learned is that I tend to do a bad job making these kinds of balancing decisions on the spur of the moment — I vastly underrepresent some areas while overworking others, so something important slips through the cracks.

打扑克？再或是读本书？我的经验就是，如果一时兴起地做出某种决择，那基本是做不好的——该做的没想到，而不该做的却做个没完，所以很多重要问题都没把握好。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

Or tuck you into bed anymore , But I still think the world of you .

我无法再将你紧紧抱在怀中，读故事给你听，或者把你塞进被窝。

I don't wanna be like Cinderella When I was just a little girl,My momma used to tuck me into bed and she read me a story .

我不想再像灰姑娘当我只是一个小女孩，我的妈妈用来抱膝我到床上，她读了我的故事。

Another colorful book which fleshes out in a highly readable fasion is Vermeer: A View of Delft by Anthony Bailey.

另一个可读的丰富多彩的图书是维米尔本身：安东尼贝利有效再述了。

Then, if you wish, you can read it once again more slowly

然后，如果你想的话，你可以再慢慢地读一遍

If people are constantly reading about you, and you're overexposed, they've got no reason to go see your movies.

如果人们总是读到关于你的新闻，你过度曝光了，他们就没有理由再去看你的电影了。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。