再胶凝

After passing the condenser, the balls will be automatically removed from the CW by means of a strainer located in the CW outlet piping, and then the balls will be diverted by a recycle pump and collector which will then reinject them into the CW inlet.

胶球通过凝汽器之后，自动的由在循环水出水管道的收集器上滤网收集，再经专门的泵继续输送至循环水入口工作。

The scFv formed inclusion body in E. Coli. 8 mol/L urea was used to solubilize the inclusion body, and the scFv was then purified in two steps , including Ni 2+ chelating affinity chromatography and gel filtration.

大肠杆菌表达的单链抗体包涵体，用8 mol/L尿素裂解，经过Ni离子螯合亲和层析和凝胶过滤两步纯化后，纯度达到97%以上，再通过透析复性除去尿素。

Here, by introducing C-terminally 6xHis-tagged human Nap1 gene into Escherichia coli and exploiting the nickel-charged sepharose gel system to purify the human Nap1 protein, we show that the Nap1 enzyme function is preserved in human as in lower organisms such as bacteria and plants using succinamic acid as the substrate.

此研究中，我们将已在胺基末端接上6个组胺酸标记的Nap1基因表现在大肠杆菌中，并使用接合上镍原子的琼脂糖凝胶纯化出人类Nap1蛋白，再以丁醯胺酸作为受质，证明人类Nap1蛋白具有酵素活性。

We amied the BCP sequence and the preS2 gene sequence of HBV separately as the target region, according to HBV DNA sequence of Chinese strain, synthesized the set of specific primers, amplified the sequence by PCR method from the serum of patients with CHI, verified by restriction analysis, subcloned into pGEM Teasy vectors, employed polyacrylamide gel electrophoresis to display the deletion mutation and selected the clones with differential length to be sequenced.

本研究应用PCR技术及其他分子生物学技术，分别以HBV转录调控序列BCP以及表面蛋白编码序列pre S2为靶基因，以中国株HBV基因序列为依据，设计特异性多聚酶链反应引物，分别自43例、51例慢性HBV感染患者血清中扩增出目的片段，构建质粒Teasy-BCP和Teasy-pre S2，酶切鉴定后，采用聚丙烯酰胺凝胶电泳技术展示缺失突变，再经DNA测序以了解在慢性乙型肝炎患者体内的病毒变异情况。

This product is made of fresh aloe gel as moisturizing lotion and equipped with high-ranking fruit acid of whitening essence and arbutin as brand-new spot-removing raw material. It is able to produce strong inhibition and whiten skin by re-using the principle of fruit acid changing skin without usual bleach or decolorization during the process of tyrosine enzyme turned into Dopa pigment C after skin absorbs it, so it brings much better effect when it is used for a long time.

产品名称：白美人祛斑霜品牌：Pamini白美人规格：20g/瓶*100瓶/件产品说明：本品以鲜芦荟凝胶为保湿剂，配以高级美白素果酸和新型祛斑原料熊果苷经皮肤吸收后对酪氨酸酶转化为多巴色素C的过程，能产生较强的抑制作用，再利用果酸换肤原理达到增白效果，并非一般漂白或脱色作用，长期使用效果更好。

Oil body is an important constituent of the seeds. In this report, oil body was isolated from the seeds and used to extract proteins.

从小桐子种子中分离油体，再从油体中提取蛋白，并采用聚丙烯酰胺凝胶电泳对油体蛋白进行分析。

We biopsied 1-2 single blastomere from 6-8 cell cleavage stage intracytoplasmic sperm injection embryo, and using nested PCR amplified the high frequently mutation region G380R of FGFR3 gene of the single blastomere. The products of PCR were digested by restriction enzyme Bfm Ⅰ, then the digested products were detected by 10% PAGE to see whether the embryo inherted the mutation of the patient and to screen out normal embryo transfer.

活检经胞质内单精子注射(intracytoplasmic sperm injection，ICSI)授精的胚胎发育至6～8细胞期的1～2个单卵裂球，采用巢式PCR扩增单卵裂球的FGFR3基因的高发突变位点G380R区域，用限制性内切酶BfmⅠ消化巢式PCR的内扩增产物，再经10%聚丙烯酰胺凝胶电泳检测有无遗传患者的该种突变，从而筛选出正常胚胎移植。

Through comparing exon sequences at both ends of introns of CHD genes of jungle fowls and cygnets, exons on two ends thereof are respectively designed with an upstream primer and a downstream primer: Psf: 5'CAAGGATGAGAAACTGTGCAA3'; and Psr: 5'GAATATCTTCTGCTCCTACTG3'; and the Psf/Psr primers are used for amplifying the CHD genes on Z and W chromosomes of meat pigeon feather pulp genome DNA, and finally the sex is identified according to the band type through 10 percent polyacrylamide gel electrophoresis and silver staining, wherein, female shows two bands and male shows one band.

通过原鸡和小天鹅CHD基因内含子两端的外显子序列比对，在两端外显子上分别设计上下游引物：Psf：5'CAAGGATGAGAAACTGTGCAA3'；Psr：5'GAATATCTTCTGCTCCTACTG3'；再以Psf/Psr引物扩增肉鸽羽髓基因组DNA的Z和W染色体上的CHD基因，最后经过10％聚丙烯酰胺凝胶电泳、银染，根据带型判断性别，其中雌性为两条带，雄性为一条带。

Crude solution of chitosanase is obtained by fungal fermented filtrates firstly, and then through affinity adsorption by cross - linked chitosan resin, with no protein flowing by suffer, and then through eluting by 1-5per mill Cu2 . After that, eluent is dialysed and concentrated and then separated by gel filtration chromatography.

将产壳聚糖酶真菌发酵液离心得粗酶液，经交联壳聚糖树脂亲和吸附，用缓冲液洗至无蛋白流出，再以1-5‰的Cu 2 洗脱，洗脱液透析浓缩后用凝胶过滤层析进行分离，获得电泳纯的壳聚糖酶。

Since the allele ranges of any two duplexes were at least partially overlapping in order to separate the alleles of four microsatellites on the same gel, the second pair had to be loaded with delay.

任何2个双链复合物的等位基因范围至少部分是重叠的，为了分离同一凝胶上4个微卫星的等位基因，第二对微卫星必须晚一段时间再加入。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。