再结合

The GPA1 gene was obtained via PCR amplification and was cloned in the two-hybrid DNA binding domain vector pGBKT7 The combinant plasmid was designated as pGBKT7-Ga.β-galactosidase assay indicatedthat Got did not have the property of self-activation. After pGBKT7-Ga was transformed to yeast pJ69-4A, we transformed Arabidopsis vegetative tissue two-hybrid cDNA library plasmids to yeast pJ69-4A containing pGBKT7-Ga.

通过PCR扩增得到GPA1基因并将其克隆到双杂交DNA结合域载体pGBKT7中，得到的重组质粒命名为pGBKT7-Gα，β—半乳糖苷酶活性鉴定表明Gα不具有自激活特性，将其转化到酵母菌pJ69-4A中，再以此为受体菌转化拟南芥绿色营养组织cDNA文库质粒。

In our study,we found thatimmunoprecipitation with an antibody to PICK1 resulted in coprecipitation of ASIC1aprotein.

在缺血再灌注的大鼠皮质组织上，与假手术组相比，等量PICK1结合的ASIC1a量有所增加。

Methods A coronoid incision plus oral incision or facial small incision were used and plastic surgery or mini splint fixation were applied in fixation or reposition for 18 patients with multiple fractures or multiple old fractures of facial bone.

方法对 18例面骨多处骨折或多发、陈旧性骨折的病人采用冠状切口加口内切口、面部小切口加口内切口相结合的术式及正颌外科、微小夹板固定技术，进行再骨折复位或截骨复位、固定。

Third, evaluate the risk of the disaster the debris flow brought. Confirm evaluation area on danger area basis, then evaluate the fatalness, damageability, loss of the debris flow. Finally, combining with foregoing research, put forward an economical and effective preventing and curing scheme for the debris flow happened in Qiongshan ditch, moreover, evaluate the projects benefit.

然后对此沟泥石流所造成的灾害进行风险评估—根据泥石流的危险范围确定评估范围，再进行危险性评价、易损性评价和灾害破坏损失评价；最后结合前面的研究，对邛山沟泥石流提出经济合理的防治方案，并对其进行防治工程效益评价。

Both polymer and non-polymer forming starter were used in the yogurt.Cultured broth was digested for 16h at pH 7.5, 30 with Pronase, precipitated with 95% ethanol, centrifugation (12000*g, 20min)and dialyzation, thus get the crude EPS, HPLC was choosed to determine the EPS composition in the cultured broth.

将产粘菌株与普通酸奶生产菌结合用于制作酸奶，用链霉蛋白酶在pH 7.5，30℃条件下消化16h，再用95％乙醇沉淀、离心、透析获得粗多糖，HPLC法测定发酵液中的糖组成及EPS的单体组成。

This system mainly contains the front-end pulse interception and back-end software analysis. In the first part, we used a non-invasive infrared measurement method based on the dual channel simultaneous measurement technique. We measured the photo-plethysmograph from the subject's fingers and toes simultaneously, then used an analog circuit and central control unit to process the signals. Finally, the digital volume pulse data is processed by a personal computer. Because the characteristic points of digital volume pulse always occur in sharp variations, this paper proposes two algorithms to solve this problem. First, we find the pacemaker and percussion wave peak from transient slope variation, and then locate the dicrotic notch and dicrotic wave peak by using the wavelet transformation algorithm. When we analyze the data, we use the Visual Basic graphical user interface, which together with the Matlab is the core of the system.

本系统主要包含前端脉波撷取及后端软体分析两部分，其中前端脉波撷取部份是以非侵入式的红外线量测方式，双通道同步量测受测者手指与脚趾的光容积变化描记图，同时将此讯号经类比处理电路及中央控制单元输出成为数位容积波，再传送至个人电脑中；而在后端软体分析部份，因数位容积波之特徵点皆出现於波形中急剧变化的部份，故本论文以瞬间斜率变化量来定位脉波讯号中的起搏点及主波峰顶点，并利用小波转换演算法（wavelet transformation algorithm）来分析重搏切迹与重搏波顶点；程式撰写主要以Matlab为核心，结合Visual Basic的图形化使用者操作介面，增加系统与使用者间的亲和力。

In this paper, several traditional models for computing are introduced at first. Conferring probability theory, the load boundary condition of that collector is analyzed, the characteristic values of Tubesheet in different design reference periods are computed. Based on homogenization method, after lots of numerical experiments, the mechanical characteristic of Tubesheet controlled different structure parameters is studied, and an empiristic formulas that could simplify Tubesheet to stiffen plate is gained. Then, G-M method is employed and Tubesheet is simplified to a normal plate.

本文在总结传统计算方法的基础上，参考概率设计方法，分析了大型袋式除尘器的荷载边界条件，计算了不同设计基准期下的花板可变荷载标准值，结合均匀化方法，通过大量数值实验，得出了不同参数对花板受力性能的影响规律，并拟合了将花板简化为加劲板的经验公式，再参考正交异性板法，最终推导了花板简化为均匀平板的经验公式。

With respect to the image blending, in order to achieve a seamless mosaic image with both the shape and exposal differences, an improved image mosaic algorithm is proposed based on the morphological method.

该算法先自适应地选取配准区域，再以互信息为相似度评价标准，结合自适应模拟退火和多分辨率搜索策略分别进行图像平移和旋转参数的全局优化和局部搜索，最后实现图像的精确配准。

The thesis is based on income question, forest coverage rate and grop production.to beging with, date envelopment analysis is proved that it can be applicated into grain for green in shan-bei district, and then in view of the fact, seven esential factors which have influence on the project are found out: expense of dam, expense of crop,expense of cash tree ,expense of defense tree,other economic crop,expense of grass and graziery;and the output factors include:income of gdp,pure income per captia,the area of decreasing land loss,graziery income ,crop production,income of tree,the totle income of economic crop.after the date of each facts are puted into dea model,unefficiencial decision making units found.the data that are got through adjusting unefficiencial dmus dy dea can offer guide in shanbei district upgrading of an industrial structure.at the same time ,taking into account some possible problems in or after grain for green in shan-bei district,the thesis bring out some propesal to improve the circumstance,enhance the life level and putthe relation of population,resource and circumstance into a healthy orbit.

论文以解决陕北地区的收入问题，植被问题以及粮食产量问题为出发点，首先从理论上分析了包络分析方法在陕北地区退耕还林中运用的可行性，然后结合陕北地区目前的实际情况，综合分析出影响陕北地区退耕还林的关键的七个因素作为输入指标：水利水保设施投入，农作物投入，经济林种支出，防护林支出，其它经济作物，草类支出，畜牧业投入；以退耕还林所要解决的最终问题作为输出指标：GDP收入、农民人均纯收入、水土流失减少量、牧业总产值、粮食产量、林业总产值、其他经济作物总产值。将各指标所对应的数据代入模型后，通过分析求解得出非有效的决策单元，再运用包络分析方法的一些基本原理对非有效的决策单元进行调整，由此所得的数据对陕北地区的实际投入具有很好的指导意义。同时，又针对陕北地区在退耕还林过程中以及之后可能面临的困境，提出了一些建议和对策。最终使得陕北地区的投资环境尽快得以改善，使农民尽快脱贫致富，使人口、资源、环境的关系尽快纳入良性循环的轨道上。

Union releases the website of information having reward among game at present, plus look be like homonymic domain name, the increase of home of Chinese net amuse oneself is dangerous!

结合现在游戏当中发布有奖信息的网站，再加上看似同名的域名，中国网游玩家的利益危险！

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。