再结合

Abortus, amplify BP26 gene by PCR and clone to pMD18-T simple vector. Identify the constructed recombinant plasmid pMDBP26 by sequencing, then subclone to vector pET-28a. Transform the constructed recombinant plasmid pETBP26 to E. coli BL21(DE3) for expression under induction of IPTG. Purify the expressed product by histidine-binding resin column chromatography and identify by Western blot.

提取布鲁氏菌基因组DNA，用PCR扩增出BP26基因，并克隆到pMD18-T载体上，测序正确后，再亚克隆至pET-28a载体上，转化到大肠杆菌BL21(DE3)中，用IPTG诱导表达，经组氨酸结合树脂柱纯化，并对纯化后的表达产物进行Western blot鉴定。

It is found that the fine tempering sorbite is obtained in condition of 930℃ direct quenching temperature and 640℃ tempering temperature after controlled rolling.

结果表明：经奥氏体再结晶区和未再结晶区两阶段结合控制轧制后在线930℃直接淬火、640℃回火，钢板可获得均匀、细小的回火索氏体组织，其综合力学性能均超过标准要求。

It is found that the fine tempering sorbite is obtained in condition of 930℃ direct quenching temperature and 640℃ tempering temperature after controlled rolling. The mechanical properties of SPV490 steel plate are above the standard requirement.

结果表明：经奥氏体再结晶区和未再结晶区两阶段结合控制轧制后在线930℃直接淬火、640℃回火，钢板可获得均匀、细小的回火索氏体组织，其综合力学性能均超过标准要求。

Critical C/Fe ratio as a new concept has been put forward in this paper, when the amount of carbon adding into pellet large than critical C/Fe ratio, it will results that the S% of DRI goes beyond the criterion so that EAF refuse to use it, when less than critical C/Fe ratio, it will accelerate reduction and desulphur.

文章根据热态DRI的再氧化机理，提出了一种利用半熔融密封料覆盖反应罐口的防再氧化理论，在还原温度时半熔融密封料熔化成液相，将罐口密封，反应罐冷却时，这层半熔融密封料凝固并和罐壁紧密结合，阻止氧化性气体的侵入，达到防氧化的目的。

In design teaching, the teaching characteristics of design major should be integrated to strengthen the training of hand molding ability, intensify the process of students' visualization consideration, reobservation, rediscovery and recreation for the design objects, which improves the students' abilities of finding problems, observing problems and analyzing problems and finally foster the students' abilities of hand molding and design.

在设计教学中，应该结合设计专业的教学特点，加强手工造型能力的训练，不断强化学生对设计对象的形象化思考和再观察、再发现和创造的过程，从而提高学生观察问题、发现问题、分析问题的能力，增强学生手工技能和设计修养。

Shanbei barreling and Yangko in a specific geographical,social and cultural background come together,and go from a combination of integration to development,and then to the historical process of separation.

陕北腰鼓与秧歌在一个特定的地域和社会文化背景下走到一起，并经历了一个由结合到融合再到发展，然后再走向分离的历史过程。

Second, we discuss two materials which are cinnamoyl chloride and azobenzene. The cinnamoyl chloride can induce the photo-dimerization reaction under linear-polarized UV light. Similarly, azobenzene can induce the cis-trans photoisomerization under UV light.

第二部分采用光诱导配向的方式，将偶氮苯与PI结合，再利用偏极化紫外光曝光使其产生顺-反异构化反应，再用polarized-ATR-FTIR研究其三度空间之分子排列，做为未来显示器配向膜的参考。

Based on analysis and compare of all kinds of precept-sorting of feature of plastic product for the different structural plastic product, a 3D feature library of plastic product was built on the platform by integrating Feature-Modeling technique with parametric technique. The feature schleps its information of non-geometry by database-externed technique of the entity. After building the database of plastic material, the module of choice of plastic material is developed by technology of ODBC. The plastic product can be sculpted by calling of feature of plastic product and inputting parameters interactively.

针对不同结构类型的塑件，在全面分析、比较塑件特征分类的各种方案的基础上，采用三维特征建模技术和参数化技术，建立了注塑产品的三维特征库；应用实体扩展数据库技术，使特征携带非几何信息；再在建立塑料物性数据库的基础上，采用ODBC技术，开发了塑料材料选择模块；通过塑件特征的调用和人机交互输入参数的方法进行塑件三维造型，再把塑件的三维实体和塑件的非几何信息结合起来，生成塑件全信息模型。

CIS-depth analysis of this paper, the essential feature of the theory and application of the law, and a new perspective based on their re-cognition, and then to the nature of chain restaurants and objective characteristics of demand for services, will be re-integrated with the CIS system organic combination of operating mode, and finally integrating theory with practice, to "real effort" for example, followed by an analysis of the various sub-integrated identification system in the restaurant chain operating in the application of a systematic, comprehensive, consistent integration of application system, so as to China's catering chain enterprises in the development of a more effective and more industry support of the theory.

本文通过深入分析CIS理论的本质特征以及应用规律，并基于新的角度对其进行了再认知，然后再以餐饮连锁经营的本质特征和客观需求为依据，将重新整合后的CIS体系与之运营模式相结合，最后理论联系实际，以&真功夫&为范例，依次分析了整合后的各个子识别系统在餐饮连锁经营中的应用，形成了系统、全面、一致的整合应用体系，从而为我国餐饮企业的连锁化发展提供了更有效的、更行业化的理论支持。

The efficiency of re-extraction using reused EDTA reached the original level of chelating extraction only for sludge B.

Fe置换螯合铜后之DTPA悬浮液可再利用於萃取A、B或C污泥中之重金属，而Fe置换螯合铜后之EDTA悬浮液则仅可再利用於萃取B污泥中之重金属，这是B污泥中重金属结合型态多为易萃出之可交换态与碳酸盐态所致。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。