再结合

Protease treatment of the plasma membranes could abolish the binding but NaIO_4 and glycosidase could not, indicating that nsLTP144 bound to plasma membranes protein without carbohydrate moiety. Using the homobifunctional cross-linking regent bissuberate (BS~3) and rice plasma membranes incubated with ~(125)I-Trx-nsLTP144, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, a putative protein receptor on the rice plasma membranes with the molecular mass around 60 kDa. NsLTP144 can not trigger extracelluar alkalization in arabidopsis, but can abolish the extracellular alkalization effect of phytopathogen elicitor cryptogein, suggesting that cryptogein and nsLTP144 may bind to the same membrane protein. In vitro pull-down assay showed that nsLTP144 interacted with OsCaM1, a possible extracellular calmodulin, implying that nsLTP144 and OsCaM1 could function in the same signal transduction pathway. These results shed light on revealing the roles of nsLTP in vivo and make it promising to finally characterize the plasma membranes receptor of nsLTP.

发现~(125)I-Trx-nsLTP144、~(125)I-Trx-nsLTP110与水稻细胞质膜均具有特异性结合，而且结合是饱和性的、可被竞争的，符合配体-受体结合的典型特征，同时用于对照实验的蛋白质~(125)I-Thioredoxin没有此特性，表明水稻细胞质膜上存在nsLTP的受体；利用可氧化糖基的NaIO_4和水解糖基的N\'-糖苷酶F处理水稻细胞质膜，再进行结合实验，结合活性几乎不受影响；而利用胰蛋白酶处理细胞膜则使得结合能力几乎完全丧失，表明其受体为没有经过糖基化修饰的蛋白质；利用交联剂BS~3交联配体一受体后，再进行SDS-PAGE分离和放射自显影，结果显示水稻细胞质膜上的nsLTP受体中有一个60kDa的蛋白质可以与nsLTP144发生特异性的结合，可能是其受体；细胞外碱化实验表明，nsLTP144不能促使拟南芥原生质体细胞培养液的细胞外碱化反应，却能猝灭来自植物病原菌的激发子Cryptogein刺激拟南芥原生质体产生的细胞外碱化反应，表明nsLTP和Cryptogein结合细胞膜上相同的位点，保护了植物细胞免受Cryptogein导致的细胞程序性死亡，并诱导系统获得性抗性的产生；体外Pull-down实验表明，nsLTP144和水稻的OsCaM1具有相互作用，暗示了nsLTP144和OsCaM1可能同在一个信号通路上起作用。

Use reseau computation to realize the data processing of system of information of modern number city, it is the process of data processing technology and computer technology recombine, it is the new thinking of digital city information processing.

利用网格计算实现现代化数字城市信息系统的数据处理，是数据处理技术与计算机技术再结合的过程，是数字城市信息处理的新思路。

A unique enzyme warm bath, using the natural fermentation heat, from the inside out to improve the body temperature, promoting blood circulation and sweating, combined with specially designed thin oil Aromatherapist superb massage excess moisture and toxins from the body.

独特的酵素温浴，利用自然发酵的热量，从内而外的提高身体温度，促进血液循环和发汗，再结合特制的瘦身精油配合芳疗师精湛的按摩手法将多余水分和毒素排出体外。

This paper describes a new method for constructing a rough neural network. In this network, rough neurons lie in the hidden-layer, and they consist of three parts which generated by two hyperplanes that partition the universe.

本文根据支持向量机可以解决小样本学习问题的优势，再结合粗集理论对不确定性问题分析的特点，提出一种支持向量机的粗糙神经网络的构造方法。

To cope with this problem, we used gamma inversion to rule out the low velocity anomalies that caused by incr...

针对这种情况，采用伽马反演，来排除因泥质含量增加形成的低速异常现象，再结合速度反演，较准确地预测出了双家坝地区飞仙关组鲕滩的发育情况，落实了其分布范围，为下一步勘探提供了可靠的资料。

In order to distinguish these two types of transitions, a state recognition method is introduced. We firstly propose a variable contact set in which the contact points on the robot body are defined to represent the alternation of the contact states logically. Then, by predefining thresholds for the contact forces, the state transition can be well recognized.3 As another research topic, the local tracking control according to the pre-acquired state transition trajectories plays an important role in realizing the non-regular motion for the humanoid robot.

在状态转换识别方法上，本文提出了接触点集合的概念，对接触状态的变化进行逻辑上的表达；再结合接触点集合的变化情况，通过设定接触力阀值来实现对状态转换的识别。3根据给定的状态转换轨迹进行局部的跟踪控制，是本文实现类人机器人非规则运动的另一个重要研究内容。

Consider dual management system as a breakthrough point,this paper analyzes Chinese Consumer Association's role positioning with sticking closely to three basic characteristics and repositions Chinese Consumer Association's role by combining the national conditions.

本文以双重管理体制为切口，紧扣社会团体的三大基本特征对中国消费者协会的角色定位展开分析，再结合我国国情，重新定位中国消费者协会的角色。

Aiming at the currently shortage of XML streams quality detecting, this paper proposes a new forecasting method of XML streams quality by least squares support vector machines, which is used the method of XML keys' vector matrix as windows, and vector product wavelet transform to multilevel decompose and refactor the XML streams series, that can fulfill real-time checking demand of XML quality, and ensure constraint, consist- ency and integrality. For even more adapting net load, it proposes a control strategy by weight and adaptive adjustment to ensure XML streams quality.

针对当前XML数据流质量检测存在的不足，提出构建XML键的矢量矩阵作为窗口，利用矢量积小波变换多级分解与重构XML数据流，再结合最小二乘支持向量机对XML数据流质量进行预测的一种方法，满足XML数据流质量重构时实时检测的要求，保证XML数据的约束性、一致性与完整性；为了更好的适应网络负载，采取加权与自适应窗口调整等调度策略充分保证XML数据流的质量检测。

Since the approach has been coded into the program, if it can be combined with the predication system of the nautical almanac data, the combined two systems can be further developed in a commercial package and dramatically improve the training or education of the current celestial navigation.

本法因已将理论透过数值计算，若能再结合航海历资料库预测系统，未来将可进一步发展成商用套装软体，改善目前商船教育训练或海上实务作业上天文航海的现况。

East and West Germany were reunited in 1990 after 45 years of separation following the end of World War II.

东德和西德再结合是在1990年，跟随第二次世界大战结束的45年之后。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。