再经过

Firstly, we would investigate wetting ability of metal chelators in order to sure cleaning uniformly. Next, we would investigate corrosion rate for copper with metal chelators.

然后我们再探讨这两种螯合剂对铜金属的蚀刻率，以确保晶圆经过清洗制程之后，铜导线表面没有腐蚀现象发生。

Through comparing exon sequences at both ends of introns of CHD genes of jungle fowls and cygnets, exons on two ends thereof are respectively designed with an upstream primer and a downstream primer: Psf: 5'CAAGGATGAGAAACTGTGCAA3'; and Psr: 5'GAATATCTTCTGCTCCTACTG3'; and the Psf/Psr primers are used for amplifying the CHD genes on Z and W chromosomes of meat pigeon feather pulp genome DNA, and finally the sex is identified according to the band type through 10 percent polyacrylamide gel electrophoresis and silver staining, wherein, female shows two bands and male shows one band.

通过原鸡和小天鹅CHD基因内含子两端的外显子序列比对，在两端外显子上分别设计上下游引物：Psf：5'CAAGGATGAGAAACTGTGCAA3'；Psr：5'GAATATCTTCTGCTCCTACTG3'；再以Psf/Psr引物扩增肉鸽羽髓基因组DNA的Z和W染色体上的CHD基因，最后经过10％聚丙烯酰胺凝胶电泳、银染，根据带型判断性别，其中雌性为两条带，雄性为一条带。

Cleverly included in each district was an urban, a rural and a coastal area , each further subdivided into parishes demes

每一个部落区都经过合理的布局，既有城区、乡区，还有沿海区，每个区下面再分为不同的教区。

The amplified fragment was inserted into pRSET-A to generate the first double repeat of the precursor gene. By utilizing a pair of isocaudamer BamH I and Bgl II sites, and another downstream Hind III site of plasmid pRSET-A, following a series of simple double digestions and ligation of the resulted products, a series of repeat (3, 4 and 6) precursor peptide fragment genes were derived.

本实验设计一对两侧含编码疏水性氨基酸密码子的引物，经过扩增前导序列10~34aa基因序列，并重新克隆入质粒pRSET-A构建串联二聚体后，再利用质粒pRSET-A的BamH I / Bgl II同尾酶克隆位点，经一系列简单的酶切和连接，快速构建这一前导肽中不含组氨酸标签序列的串联多聚体基因，并成功表达其六聚体重组蛋白。

I have been in all my Circumstances a Memento to those who are touch'd with the general Plague of Mankind, whence, for ought I know, one half of their Miseries flow; I mean, that of not being satisfy'd with the Station wherein God and Nature has plac'd them; for not to look back upon my primitive Condition, and the excellent Advice of my Father, the Opposition to which, was, as I may call it, my ORIGINAL SIN; my subsequent Mistakes of the same kind had been the Means of my coming into this miserable Condition; for had that Providence, which so happily had seated me at the Brasils, as a Planter, bless'd me with confin'd Desires, and I could have been contented to have gone on gradually, I might have been by this Time; I mean, in the Time of my being in this Island, one of the most considerable Planters in the Brasils, nay, I am perswaded, that by the Improvements I had made, in that little Time I liv'd there, and the Encrease I should probably have made, if I had stay'd, I might have been worth an hundred thousand Moydors; and what Business had I to leave a settled Fortune, a well stock'd Plantation, improving and encreasing, to turn Supra-Cargo to Guinea, to fetch Negroes; when Patience and Time would have so encreas'd our Stock at Home, that we could have bought them at our own Door, from those whose Business it was to fetch them; and though it had cost us something more, yet the Difference of that Price was by no Means worth saving, at so great a Hazard.

一般人往往有一种通病，那就是不知足，老是不满于上帝和大自然对他们的安排。现在我认识到，他们的种种苦难，至少有一半是由于不知足这种毛病造成的。患有这种病的人大可以从我的一生经历中得到教训。就拿我自己来说吧，正是由于我不满自己原来的境况，又不听父亲的忠告--我认为，我有悖教训，实为我的&原罪&，再加上我后来又犯了同样的错误，才使自己落到今天这样悲惨的地步。当时，造物主已安排我在巴西做了种植园主。如果我自己不痴心妄想发财，而是满足于逐渐致富，这时候我也许已成了巴西数一数二的种植园主了，而现在我却白白地在这荒岛上流落了这么多年，过着悲惨孤寂的生活。而且，我在巴西经营时间不长；就是在这段短短的时间里，我也获利不少。因此我确信，要是我继续经营下去的话，到现在一定拥有十几万葡萄牙金币的家财了。当时，我的种植园已走上了轨道，并且日益兴旺。可是，我偏偏把这一切丢弃，甘愿去当一名船上的管货员，只是为了到几内亚去贩卖黑奴。现在想来，我为什么要这样做呢？要是我守住家业，只要有耐心，经过一段时间之后，同样可以积聚大笔财富，我不是也可以在自己的家门口，从那些黑奴贩子手里买到黑奴吗？虽说价钱贵一点，但这点差价绝不值得自己去冒这样大的风险！

To detect the position of crop-rows, a method based on Hough transform and connected component labeling was put forward. First, a true color image of field crops was processed with gray histogram and OSTU respectively. Then the noises in the image were eliminated by means of eight connected component labeling according to their areas. The centroid coordinates of each labeled crop were computed after the second labeling, through which the center line of the crop-row was regressed by Hough transform. Experimental results show that the method is effective.

该方法分别用直方图法和最大类间方差法二值化图像后，经过8连通区域标记后据其面积属性去除噪声，然后再经一次标记后找出各标记作物的重心点坐标，最后通过Hough变换检测过重心点的直线，即到得到过作物行的中心线，试验结果表明了该方法的有效性。

Failure is the mother of success, we have to pass the flections and frustrations of life growing up, constantly falling, came step by step and slowly realise of it!

失败乃成功之母，人生的成长路是经过曲折和坎坷，不断跌倒再爬起来，一步步走来，并慢慢领悟的！

He'd cross-referenced the soft blues and golds of Vermeer and the elaborate lace collars and sleeves of Van Dyck with typically Galliano-esque hyper-exaggerations of Dior's tight-waisted, full-skirted fifties shapes.

这次，他糅合了维梅尔式的淡蓝和金黄、凡·代克式的精美蕾丝高领，再加上经过Galliano标志性极度夸张的、Dior家的收腰设计，以及五十年代正式裙装的轮廓。

In Iowa City, deciding to cut down another one of its few remaining ginkgoes was a no-brainer.

在爱荷华市银杏树已所剩无几，不过要再多砍几棵，也不需经过太多考虑。

Whisky, refers to a broad category of amber color alcohol that are distilled from fermented grain mash and aged in oak casks, the word 'whisky' is from the Scotch Gaelic ' uisge-beatha ', which means 'water of life'.

威士忌酒是以大麦芽和其它谷物为原料，加酵母菌发酵，经过蒸馏获得烈性酒，再经木桶长期贮存、陈化而成。酒呈琥珀色，味微辣而醇香。威士忌这个字是来自苏格兰古语 GAELIC 中的&UISGEBAUGH&或&UISCEBEATHA&，其意思是。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。