再组织

Objectives: To observe the influence of Danshen to the expressions of mRNA and protein of local hypoxia inducible factor-1α(HIF-1α) during acute ischemia reperfusion injury of skeletal muscles in SD rats, and its effects on the changes in erythrocyte aggregation index, erythrocyte deformation index, local swelling coefficient and histology of the left cremaster muscles of SD rats during ischemia reperfusion injury, therefore to discuss the influence of Danshen to the oxygen environment and hemorheology of skeletal muscle during ischemia reperfushion injury.

目的：通过建立SD大鼠左侧提睾肌的缺血再灌注损伤模型，观察丹参对SD大鼠骨骼肌缺血再灌注损伤局部缺氧诱导因子1α（Hypoxia Inducible Factor-1α，HIF－1α）mRNA及其蛋白表达的影响、对红细胞聚集和变形指数的变化、局部肿胀系数的变化以及组织形态学改变的作用，探讨丹参对缺血再灌注损伤骨骼肌局部氧环境及血液流变性的影响。

METHODS: A limb ischemia-reperfusion injury model in rats was established. The animals were randomly divided into three groups: control group, IR group and IPC group. The contents of diamide oxidase, nitric oxide, endothelin-1 (ET-1) and ratio of nitric oxide/endothelin-1 (NO/ET-1) in the plasma and the gut were measured. The leavels of myeloperoxidase, ratio of DNA chain, total nitric oxide synthase, inducible nitric oxide synthase and constitutive nitric oxide synthase in the gut were determined.

雄性Wistar大鼠18只，随机分为对照组，缺血再灌注组和缺血预适应组，每组6只，分别测定血浆和小肠组织二氨氧化酶、一氧化氮、内皮素-1(ET-1)、NO/ET-1比值的含量变化及小肠组织的髓过氧化物酶、DNA双链百分率(ratio of DNA chain %)、总一氧化氮合酶、诱导型一氧化氮合酶、结构型一氧化氮合酶的水平；免疫组化法检测小肠组织的诱导型一氧化氮合酶、内皮型一氧化氮合酶的表达。

So, we use Theory of Constraints to diagnose organization's internal constrain and expect the TOC will be succeed to solve the organization's problems. On the other side, in the make-to-order factors the Production Control department is the most critical key for the coordinate with Production and Sales. But we found the Production Control department hadn't played the role of coordinate by appropriately because they have some problems what hadn't solved till now yet in this case.

另外，在接单式生产的工厂里，生管部门是非常重要的单位，在本研究分析订单交期不准时的过程中发现，生管部门在整体运作上也存在著一直未被改善的问题，所以本研究也将依循著限制理论的思维程序，带领生管同仁一同参与组织问题的诊断，透过工作问题的收集找出组织的内部限制，再一起研拟改善对策打破限制，进而完成生管部门组织问题改善。

At the same time,the protective effect of mannital and VitC were assessed.Result:The serum level of creative phosphokinase and malon dialdehyde was higher,but superoxide dismutase activity was lower during reperfusion than in the ischemic period.The tissue structure and ultrastructure were damaged more seriously in reperfution period. After treated by mannital and VitC, the level of CPK and MDA in serum was lower, but SOD activity rasied, and the ultrastructure was better.

结果：再灌流组较缺血组血清CPK增高、MDA升高，再灌流组脑组织中MDA 升高，血清中SOD活力下降，再灌流组脑组织结构和超微结构破坏更加严重，应用自由基清除剂后血清中CPK降低，MDA含量降低，SOD活性升高，组织中MDA含量亦降低，超微结构较前好转。

Objective: The modern medical research thought that the cerebral ischemia- reperfusion injury is an important pathophsiology mechanism that causes the various cerebral disease,and apoptosis is one of the important mechanisms of the cell death in the process of the organ and the organization ischemia-reperfution.

目的：现代医学研究认为缺血再灌注损伤是大多数急性缺血性疾病的主要病理生理过程，而细胞凋亡则是器官、组织缺血再灌注损伤过程中细胞死亡的重要机制之一，因此干预细胞凋亡，从而防治缺血再灌注损伤已经引起国内外广泛关注。

Results showed that green firm callus and rooting were obtained with treatments of NAA 0.5-3.0mg/L, in which the NAA 0.5mg/L treatment appeared the optimal rooting result of 90%. White loose callus was obtained with treatment of 2,4-D0.1-3.0 mg/L.Callus can not be induced by adding BA solely at 0.5-3.0mg/L. There appeared bud redifferentiation only when appropriate concentration of combined BA,NAA and 2,4-D were applied.

结果表明：单独加入NAA0.5～3.0mg／L可诱导出绿色致密的愈伤组织，并再生出根，其中NAA0.5mg／L处理生根率最高，达90％；单独加入2，4-D0.1-3.0mg／L可诱导出白色疏松的愈伤组织；单独使用BA0.5—3.0mg／L不能诱导出愈伤组织；BA与NAA或2，4-D只有在适当的质量浓度范围内配合使用才能分化出芽，芽分化率最高的处理为BA3.0mg／L＋NAA0.5mg／L，分化率为30％。

Methods: Single lung in situ ischemic reperfusion animal model was used. 30 rabbits were randomly divided into three groups: sham group, ischemic/reperfusion group and ischemic preconditioning group. Wet to dry ratio and injured alveoli rate were determined. Ultramicroscopic structural change was observed under electron microscope. Heat shock protein-27 was detected with immunohistochemistry.

采用在体兔单位缺血瑞灌注模型，30只日本大耳兔随机均分为三组：假手术组、缺血／再灌注组及缺血预处理组，实验检测肺组织混干重比和肺泡损伤数比值，电镜评定肺组织起微结构损伤，免疫组化检测肺组织热休克蛋白27的定位及表达。

Histomorphology changes of liver tissue were observed with light microscope.

再灌120min后，各组取血及肝组织，测定血清及肝组织中SOD活力、MDA和NO水平，光镜下观察肝组织形态学改变。

Objective To research effect on tendon healing and adhearing after tendontransplanted with vascular vessel and protect paratenonMethods 30 rabbits were regarded as study objects ,the double Achillestendons were cut off and anastomosd in 24 ,the left Achilles were anastomosedwith protecting paraneton and circulation under microscope as theexperimental group; the right Achilles were handled as the left exceptresecting the peritendineum as the control group; the another 6 as thenormal group without any surgical intervene. All the animals wereimmobilized with plaster 3 weeks.

目的 研究吻合血管、保留腱膜肌腱移植对肌腱愈合及粘连影响方法以30只健康中国白毛红眼兔为实验动物，双后肢自身对照，实验组取左后肢于镜下保护好腱周血供，在腱周组织表面用尖刀片轻柔作一约0.5cm切口，挑出一束跟腱刀片横断，3-0无创缝线改良Kessler法缝合，再间断缝合腱周组织；对照组取右后肢，游离并切除腱周组织，余操作同实验组；空白组：不予任何外科干预。

Tissue engineering is a multidisciplinary science merging with cell biology, material science, engineering and chemistry. Its basic idea is to expand a number of cells in vitro and seed them on a polysynthetic biodegradable polymer structure, and finally enable the organization of 3-dimmentional and functional tissues or organs.

组织工程技术是融合了细胞生物学、材料科学、工程学和化学的一门边缘学科，它的基本思想是用少量组织细胞经体外培养扩增后播种在可降解的生物支架材料上，再回植入体内形成有功能的组织或器官。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。