再组织

Rats of pancephalic ischemia and reperfusion were establ- ished with gyral vessel closing method by pulsinelli. SOD and MDA in cerebral tissue, transmitter content of catecholamine in hippocampal gyrus. striatum and neocortex, and patho- morphism of cerebral tissue of experimental animals were stu- died by piercing Baihui to Qubin of rats with filiform needle.

本课题采用Pulsinelli四血管关闭方法建立大鼠全脑缺血再灌注模型，对比观察了毫针透刺大鼠百会至曲鬓穴区对实验动物脑组织中SOD、MDA，海马、纹状体、新皮层中的儿茶酚胺递质含量及脑病理组织形态等方面进行研究。

The steps are as the following:1、Preparing the cerebrospinal fluid one hour before the experiment,take some of them for cryopreservation;2、Without paralyse,cuting down the rat head quickly and dislodge the hippocamp part which is used in the experment in freezing liquid,and trim the hippocamp distrct.3、Fixing the hippocamp tissue on the vibratome,to cut down a brain slice with 300μm,use the haustorial tube to move the slice to the preincubate dish,cultivanting it for one hour.Then move it to the record incubation chamfer.4、Preparing the glass micloelectrode and fill it with NaCl,the corcentation is 3mol/L.5、adjusting the recorder,after cultivate the brain slice in the incubation chamfer for 2 hours,the expermentize can be gone on.6、In order to ensure the accurate of the experimental result,use only one medicine concentration for one brain slice in every experiment.

本实验研究方法是：1、实验前1小时配制好所需脑脊液并充以95% O2和5% CO2的混合气，取小部分冷冻保存；2、在乙醚麻醉下，快速断头并在冷冻液里取出包含海马的大脑部位，修整出所需海马区；3、将海马组织固定于振动切片机上切出300μm厚的海马脑片，用广口吸管转移至预孵育皿培养一小时，后再转移到记录用孵育槽内；4、拉制好玻璃微电极，并充以3mol/L的NaCl；5、调试好记录仪器，将脑片在孵育槽内培养2小时后进行实验；6、为了保证实验结果的准确性，每一块脑薄片只进行一个浓度的药品实验。

Four weeks after operation, a large quantity of rosy newly formed bone and green bone trabecula were showed in the BG-COL-PS-HYA group. At week 12, scaffold materials was substituted by new bone tissues basically, and Haversian system was visible. Eight weeks after operation, the results of X-ray film revealed that cortical bone was fully connected.

生物活性玻璃/胶原蛋白/透明质酸/磷酸丝氨酸组术后4周硬组织切片可见大量玫瑰红色新骨和绿色骨小梁形成，术后12周支架已基本由新生骨组织替代，哈弗系统形成；术后8周X射线显示骨皮质连接完整，12周缺损完全修复，髓腔基本再通。

However, tracheal stent implantation is different from esophageal stent implantation. As patients with airway obstruction are always complicated by dyspnea and hypoxemia, even respiratory failure, which increases risk and difficulty of stent implantation. During tracheal stent implantation, the bronchofibroscope or stent entering the stenosis site may lead to complete tracheal obstruction-induced asphyxia. Corrosion of metal wire of stent to airway mucosa and vessels may result in hemorrhea, leading to postoperative hemoptysis. Stent dislocation is mainly caused by inappropriate selection of stent, inappropriate implantation position or severe cough. Carcinoma tissue growing along stent lumens can induce re-obstruction in stent, resulting in atelectasis relapse.

气管及食管支架置入均属有创性治疗，但气管支架置入不同于食管支架置入，气管阻塞患者均有不同程度的呼吸困难和低氧血症，甚至发生呼吸衰竭，加大了支架置入的风险和难度；气管支架置入者，当纤支镜或支架进入狭窄部位时可造成气管的完全阻塞引起窒息；支架金属丝对气管黏膜血管的侵蚀可引起大出血，导致术后咯血；支架移位主要由支架选择不当、置入不到位或剧烈咳嗽引起；癌组织沿支架网眼长入造成支架内再阻塞可引起肺不张复发。

College of Veterinary Medicine, China Agricultural University, Beijing 100094, China Abstract: A method has been developed to determine residual stilbenes such as diethylstilbestrol, dienestrol and hexestrol in animal tissues using solid phase extraction and gas chromatography-mass spectrometry.

动物组织样品在碱性条件下用乙酸乙酯提取，经碳酸钠溶液液-液分配净化，再用硅胶柱固相萃取净化，洗脱液均分成两份后经氮气吹干，分别用双三甲基硅基三氟乙酰氨和七氟丁酸酐衍生，采用选择离子监测模式进行测定，外标法定量。

The Sham rats were only performed the incision on cervix but without any treatment on the arteries. Apoptosis neurons in hippocamp CA1 subregion were observed at 72 hours after global brain ischemia.

在脑缺血再灌注后72小时，杀死大鼠，取海马组织依POD法作凋亡细胞检测，计数各组大鼠海马CAI区凋亡细胞数。

This persecution resulted in groups of Anabaptists fleeing together, which in turn resulted in the formation of ethnic groups like the Mennonites, the Amish, and the Hutterites.

这些迫害导致了一群再洗礼派教徒逃亡到一起，后来又致使了门诺派，阿们宗派和哈特派等异教徒的组织的形成。

If the ratio or the concentration of 2, 4-D was higher, the callus formed quickly, but it was hygrophanous, and easy to necrosis, conversely, if the ratio or the concentration of 2, 4-D was lower, the callus appeared very slowly or could not form, and adventitious root formed directly or indirectly.

比值较高（或2，4-D浓度较高）时愈伤组织出现的时间较早，但愈伤色泽不好，呈半透明水渍状，易于褐化死亡，而比值较低时，愈伤出现得很慢或不形成愈伤，且易于直接或再分化形成不定根；这些不定根长至1.5-2.0cm时就不生长，并逐渐褐化。

Results The exoression of MMP-9 was significantly increased in hyperglycemic groups after irrigating 24h,48h post 2h ischemia.

高血糖可诱导脑缺血再灌注后脑组织内的MMP-9 表达，增加血脑屏障的通透性，从而加重脑组织的损伤。

Methods:The subcutaneous adipose tissue was obtained from the fold inguen of four SD rats, then digested with collagenase type Ⅰ and cultured in endothelial medium for seven days.

获取4只雄性SD大鼠鼠蹊部皮下脂肪，通过机械分割和组织消化法获取脂肪基质细胞，在内皮细胞培养液中培养7d后，经免疫细胞化学及透射电镜鉴定证实95%以上细胞已具内皮细胞表型特征，再将这些细胞进行成脂(DMEM/F-12培养基内加入0.5mmol/L1-甲基-3-异丁基-黄嘌呤，1μmol/L地塞米松，10μmol/L胰岛素，200μmol/L吲哚美星)定向诱导7d，形态学观察和油红O特殊染色鉴定诱导后的细胞。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。