再组织

Methods After left lateral segmental partial hepatectomy or right anteroinferior partial hepatectomy,the left external inferior or the right anteroinferior hepatic duct was exposed,and intrahepatic chlangio-jejunostomy was performed.

切除部分肝左外侧叶，解剖出左外下段支肝管或切除右前叶下段部分肝组织，解剖出右前下段支肝管与空肠行Roux-y吻合，在有效减黄率，合并症，黄疸再发率及生存期等方面与三组非外科胆管引流方法PTBD、ERBD、EMBE进行对照比较。

After ethanol extracted hepaticglycogen, it was identified by indic colorific mensuration and quantified with anthrone mensuration. Finally, experimentative design was bringed forward.

采用三氯醋酸沉淀组织蛋白质，乙醇提取肝糖原后，以碘呈色法对其鉴定，再以蒽酮法进行定量，最后提出对相关物质检测的实验设计要求。

Methods］ Healthy SD rats were divided randomly into 6 group, including: normal control group(sham-operation), ischemia for 45 min (reperfusion 0h, n=6), reperfusion 2,4,12 and 24 hr group. Renal ischemia reperfusion injury models were founded in other five groups, opened abdominal and placed atraumatic vascular champ in dual kidney pedicle for 45 minutes,followed by reperfusion 2,4,12 and 24 hr.

方法］ 将SD大鼠随机分成正常对照组、单纯缺血组、再灌注2 h组、4 h组、12 h组、24 h组，制备双侧肾蒂夹闭模型后，腹主动脉取血测血肌酐、尿素氮，行肾脏组织病理学检查，免疫组化法检测TGFβ1 蛋白，RT-PCR法检测TGFβ1 mRNA。

"China sees as an enormous market that is not claimed or controlled by any one nation, and there is an opportunity for them to do it," says Carberry."The combination of urgency; the enormous needs; a focused, systematic planned government; an army of engineers; and access to capital may define China as the platform for the green- technology industry globally."

中国认为绿色技术是一个还没有为其他国家所控制的一个巨大的市场，这个市场所具有的迫切性，经济上需要的强大性，再加上一个十分专注的有强大计划和组织能力的政府，还有一个几乎像军队一样的工程师们的队伍，同时还有资金调度方面的灵活性，这些因素加在一起就很足以构成一个在世界范围内造就一个绿色技术工业强国成长的平台。

A method for eliminating the internal oxide structure in the gas-phase carbon-nitrogen osmosis of gear features that the CCL4 is added to the kensene as carburizer and then is decomposed to generate Cl2 for reducing the oxide, the ammonia gas as nitrizing agen passes through the cylinder of silica gel or granular calcium chloride for adsorbing residual water vapour and then through the cylinder of 5A molecular sieve for adsorbing residual O2, and the resistance wire is wound on igniting pipe at furnace top to prevent the NH4Cl in exhausted gas from being condensed.

本方法采用在气体渗碳剂煤油中，加入6～8％的CCl 4 ，使共渗炉内的CCl 4 分解出新生的Cl 2 ，使已形成的合金元素内氧化物还原；在渗碳剂氨气入共渗炉前，先经过硅胶瓶或桶及粒状氯化钙瓶或桶过滤，吸附掉氨气中的残留水蒸汽，再经过5A分子筛瓶或桶吸附掉氨气中的残留氧；在靠近炉顶位置的点火管周围缠绕带瓷环的电阻丝，靠电阻丝的副带加热，使排气中的NH 4 Cl不冷凝结块等措施实现内氧化组织的消除。

Animals after chemolysis failure to homogenate respectively,centrifugate and take the pure liquid,mcasure collagenase activity with Blanckaert method,the data handled with statistics did not show obvious difference.

组织化学检测：将实验动物组、对照组、手术组和化学溶解术失败再手术组标本的纤维环内层、中层和外层分别匀浆、离心，取上清液，按Blanckaert法测定胶原酶，所得据经统计学处理差异无显著性。

The chylous leakage was stopped successfully with pledgets and Surgicel using a tie-over suture technique.

我们将缝线及小棉垫片固定於乳糜外漏附近较健康的组织，再用止血棉Surgicel填塞在外漏处并用捆扎的方式固定，成功地止漏。

The GPA1 gene was obtained via PCR amplification and was cloned in the two-hybrid DNA binding domain vector pGBKT7 The combinant plasmid was designated as pGBKT7-Ga.β-galactosidase assay indicatedthat Got did not have the property of self-activation. After pGBKT7-Ga was transformed to yeast pJ69-4A, we transformed Arabidopsis vegetative tissue two-hybrid cDNA library plasmids to yeast pJ69-4A containing pGBKT7-Ga.

通过PCR扩增得到GPA1基因并将其克隆到双杂交DNA结合域载体pGBKT7中，得到的重组质粒命名为pGBKT7-Gα，β—半乳糖苷酶活性鉴定表明Gα不具有自激活特性，将其转化到酵母菌pJ69-4A中，再以此为受体菌转化拟南芥绿色营养组织cDNA文库质粒。

In our study,we found thatimmunoprecipitation with an antibody to PICK1 resulted in coprecipitation of ASIC1aprotein.

在缺血再灌注的大鼠皮质组织上，与假手术组相比，等量PICK1结合的ASIC1a量有所增加。

Right MCAO was induced in all animals by a 4-0 nylon thread with round tip inserted cranially into right internal carotid artery and maintained for 90 min. HSP70 mono-clone antibody immunocytochemistry was used to detect the changes of HSP70 expression in rat cortex at 6, 12,18,24,48 and 72h reperfusion in conjunction with histopathological analysis of rat brain.

应用HSP70单克隆抗体免疫组化染色检测大鼠再灌注6、12、18、24、48及72h脑皮质HSP70表达的变化；并与病理组织学改变进行对照研究。2.20只雄性新西兰大白兔随机分成对照组（n=10）及葛根素组（n=10），所有动物均阻闭腹主动脉肾下段20min，建立兔脊髓腰尾段缺血模型。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。