再纯化

In contrast, deletion of the propeptide yields mature but inactive NK, the inclusion bodies of NK or denatured rNK couldn't be renatured in any condition, but with the aid of exogenously purified propeptide or Ser221Ala proNK, the inactive mature protein could be renatured by an intermolecular pathway in vitro.

此外，我们利用融合基因表达系统在大肠杆菌中高效表达了谷胱甘肽S-转移酶／NK和硫氧还蛋白／NK两种融合蛋白，经体外复性、纯化，均获得活性蛋白。以上结果显示，pro肽在体内体外，通过分子内或分子间的方式均能帮助NK分子的折叠与再折叠，作为一种分子内的分子伴侣，pro肽为NK分子正确折叠所必需。

The experiment adopt capital: Thousand layers of tower entire grass crush , the sour water logging carries , centrifugal fliteration , concentration, neutralize , the organic solvent extracts , oppose an extraction , still organic menstruum of alkali liquor extracts , concentrates , extracts except steps such as miscellaneous , fading and highly valuing crystal from the thousand layers of a tower middle draw, pure fossil China fir alkali A.

本实验采用：千层塔全草粉碎、酸水浸提、离心过滤、浓缩、中和、有机溶剂萃取、碱液反萃取、再有机溶剂萃取、浓缩、萃取除杂、脱色和重结晶等步骤从千层塔中提取、纯化石杉碱甲。

The purification method of the deoxyribonuclease comprises the following steps: drying fresh fruiting body of Ganoderma lucidum, pulverizing, extracting to obtain crude deoxyribonuclease, salting out with ammonium sulfate, carrying out affinity chromatography, gradient-eluting, carrying chromatography in SP Sephorose Fast Flow column, and gradient-eluting with 0 to 0.3 M NaCl to obtain seoxyribonuclease of Ganoderma lucidum.

该酶的提取纯化方法，以新鲜灵芝子实体为原料，经干燥处理后粉碎、粗酶抽提、硫酸铵盐析、亲和层析，梯度洗脱，再用SP Sephorose Fast Flow柱层析，以0～0.3M NaCl梯度洗脱，即可获得灵芝脱氧核糖核酸酶。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失