再纯化

The purified TGF β1 monomer protein can form into active dimeric TGF β after refolding in vitro.

纯化的TGFβ1单体蛋白通过体外再折叠可以形成具有生物活性的TGFβ1双体分子。

According to different acidity of free Flavonoids in Resina Draconis, different acidic lavonoids were extracted with the pH gradient extraction method from ethyl ether.

根据血竭中游离黄酮存在着酸性差异，采用溶剂除杂，转溶于乙醚，再用pH梯度萃取得到不同酸性的黄酮，进而对弱酸性组分分离纯化得到2个单体化合物。

Optimum Extraction of Ursolic Acid in the Residues of Eriobotrya japonica by Orthogonal Test;2. After total saponins of Panax notoginseng were extracted,residues left was refined,separated,and purified.

通过对三七总皂苷提取后的药渣进行重新提取、分离与纯化得到了三七中另一有效成分———三七多糖，经含量测定可达50%，提高了三七的再开发利用价值。

Based on biological experiments, mango glycoside is used as raw material; under the condition with phenol and hydroiodic acid, carbon-carbon bonds are broken to produce the crude product of mango aglycone; the crude product is separated and purified through silica gel column chromatography so that the mango aglycone is separated; the yield rate reaches 12 percent, the purity is higher than 95 percent, and the reproduction performance is good; the IGTT of C57BL/6 normal mice is effectively improved with the norathyriol; and simultaneously the quantity of insulin secretion induced by glucose in vivo for the C57BL/6 normal mice can be improved with the norathyriol.

通过生物学实验证明，本发明所涉及的以芒果苷为原料，在苯酚和氢碘酸条件下，使得碳-碳键断裂，制备得到芒果苷元粗品，再通过硅胶柱层析分离纯化得到芒果苷元的方法使得其产率可达12％、纯度大于95％，且重现性好；norathyriol可使C57BL/6正常小鼠IGTT有明显改善；同时norathyriol可提高C57BL/6正常小鼠体内葡萄糖诱导的胰岛素分泌量。

After extraction by ethanol and purification by cationic ion-exchange column, a method of using reversed-phase high-performance liquid chromatography and precolumn derivatization with o-phthalaldehyde and 9-fiuorenylmethylchloroformate is employed for determination of free amino acids in tobacco . This method assures that amino acids with amino and imino groups can be determined simultaneously. The results of duplication experiment and recovery experiment are acceptable.

本试验用乙醇提取烟叶中的游离氨基酸，并用阳离子交换树脂纯化，再用邻苯二甲醛、氯甲酸芴甲酯联合柱前衍生、反相高效液相色谱分析测定了烟叶中的游离氨基酸，该方法能同时测定烟叶中的带氨基和亚氨基基团的氨基酸，并且分析方法的重现性和回收率均令人满意。

Woodson or apocynum venetum Linn as raw material, it utilizes the method of decoction and alcohol sedimentation to remove impurity. After extract is separated by macroporous adsorptive resins, then use polydextran gel LH-20 to purify, obtain isoquercitrin. Compared with the method of traditional silica gel column chromatography, this method has characteristics such as: experiment period is short, dissipation of organic dissolvent is little, yield of produced product is high, and purity coefficient is fine and so on. Isoquercitrin produced by this invention can be provided for medicine research or used as chemistry control article.

Woodson或罗布麻的叶为原料，采用水提醇沉除杂的方法，提取液经大孔吸附树脂分离后再用葡聚糖凝胶LH-20纯化得罗布麻甲素，此法与传统硅胶柱层析的方法相比，具有实验周期短，有机溶媒消耗少，制得的产品得率高和纯度好的特点，本法制得的罗布麻甲素可供药物研究或化学对照品使用。

Silica gel column and reversed-phase C-18 column were employed to purify and separate monohydroxy intermediates.

此外，对DKR催化双羰基底物还原得到的3R，5S-双羟基产物进行纯化，再通过羟基保护和保护基的去除，以用于他汀类药物的手性侧链制备。

The recombinant protein was purified by Ni-NTA affinity chromatography and gel filtration chromatography. The inbred C57BL/6 strains and outbred KM strains of mice were subcutaneously immunized with the protein formulated by either adjuvant ISA720 or aluminium hydroxide.

表达产物通过Ni-NTA亲和层析和凝胶过滤层析方法纯化，再分别与ISA720和Al3佐剂乳化后，免疫C57BL/6和昆明小鼠，通过尾蚴膜反应和ELISA反应检测特异性抗体。

This operation is suitable for the following portal hypertension: simple occlusion of hepatic vein (a pathological type of Budd-Chiari syndrome)thrombosis in portal vein or prehepatic portal hypertension due to cavernous transformation; intrahepatic portal hypertension with rebleeding after treatment of splenectomy or without operation, and the patients with liver function in Child A or B class.

本手术主要适应于以下原因引起的门静脉高压：①单纯化肝静脉闭塞(布-加综合征的一种病理类型)；②门静脉主干血栓形成成海绵样变性引起的肝前性门静脉高压；③肝内型门静脉高压行断流术后再发出血或未行手术，肝功能为Child A和B级者。

β-Cyclodextrin with the 6-hydroxyls protected by dimethyl-tert- butylsilyl was selectively oxidized by lead tetraacetate. After the silyl groups were removed off by boron fluoride-etherate, mono-2,3- dialdehydro-β-cyclodextrin as a cuniform molecule could be constructed.

用四醋酸铅将七元（6-O-二甲基叔丁基硅基）-β-环糊精选择氧化、并用三氟化硼乙醚脱硅基保护基后，再经分离、纯化，可获得具有&楔筒状&空腔的单-2， 3-二醛基-β-环糊精。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失