再纯化

The best training samples are searched and selected on the whole image by the local space information and spectral matching, and then they are purified on spectral domains.

利用影像的空间信息在图像局部范围内自动搜索和选择最佳样区位置，再用光谱匹配对寻找到的最佳样区在光谱空间上进一步纯化。

The method takes microcrystalline cellulose as affinity column chromatographic medium of Beta-glucosaccharase; under low temperature and anacid condition, the sample of Beta-glucosaccharase is loaded and specifically adsorbed to the microcrystalline column, and then the concentration of NaCl is increased to elute the enzyme, so as to achieve high-efficient and economic separation and purification.

本方法以微晶纤维素作为β-葡萄糖苷酶的亲和柱层析介质，在低温和微酸性条件下，将β-葡萄糖苷酶上样并特异性吸附于微晶纤维素柱，然后再通过增加NaCl浓度的方法将该酶洗脱，从而达到其高效而经济分离纯化的目的。

Then the extract was distributed between dichloromethane and water, and the organic part was then subjected to HSCCC for the isolation of chromone constituents.

以芦荟全叶为原料，经过一系列的预处理手段，从脱色剂活性炭中获得芦荟色酮粗提物，再经过溶剂分配和富集后采用高速逆流色谱对其中的色酮成分进行分离纯化。

High-speed countercurrent chromatography is reported for the preparative isolation and purification of a chromone from aloe vera. The crude extract was obtained by a series of pretreatment of aloe vera leaves and extracted from decolorizing active carbon with methanol.

以芦荟全叶为原料，经过一系列的预处理手段，从脱色剂活性炭中获得芦荟色酮粗提物，再经过溶剂分配和富集后采用高速逆流色谱对其中的色酮成分进行分离纯化。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2+-chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2 -chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

The optimum extraction process of preparation was carried out with orthogonal test,and the craft of extraction by the single factor experiment, and I suruey the corrigent to choose suitable quantity. This result would be technology parameters for production of Shuanghuanglian Oral Liquid.

通过正交试验优选出双黄连口服液最佳的提取工艺，再通过单因素试验考察纯化工艺，并对矫味剂进行考察、筛选，从而为大生产提供合理可行的技术参数。

It is concluded that the ultrafiltration preconcentration combining with multieffect evaporation is a promising concentration procedure for straw black liquor, which can not only reduce the concentration costs, but also improve the subsequent evaporation and combustion operation due to the desilicification and purification of ultrafiltration.

两种膜比较，磺化聚砜膜更易清洗，而且水通量大。5）超滤浓缩后，黑液中木素的浓度和纯度大大提高，超滤是从黑液中分离木素的理想方法。6）与多效蒸发相比，用超滤法浓缩麦草黑液可降低能耗70~85%，若用于黑液的预浓缩，再结合多效蒸发，不仅可以降低黑液的浓缩费用，而且由于除硅和纯化作用，会大大改善后继的蒸发和燃烧的操作。

Nepalensis wall polysaccharide was prepared with hot water extraction,ethanol precipitation,dialyzation,and lyophilization.the crude polysaccharide was separated by deae-cellulose chromatography and purified by sephadex g-200 chromatography.the inhibit effect of the polysaccharide on lipid peroxidation of mice liver was conducted by tba method.the model of liver-injury induced by carbon tetrachloride（ccl4was used to validate the variety of the realated ast.results onefold ingredient polysaccharide was obtained after isolated and purified.conclusion the ingredient can remarkably inhibit lipid peroxidation of rat liver and significantly reduce activity of ast.

用热水提取，乙醇沉淀提取粗多糖，再经sevag法去除蛋白，透析去除小分子物质，冷冻干燥得楮头红多糖粗品；采用deae-52纤维素柱层析和sephadex g-200凝胶柱层析进行分离纯化；用tba法考察多糖的抗脂质过氧化作用；采用ccl4急性肝损伤模型，测定其血清中ast变化。结论获得一个楮头红多糖组分。tba法表明该组分对小鼠自发性脂质过氧化具有显著的抑制作用；在保肝试验中，给药组能明显抑制ast的升高，对肝损伤有一定的保护作用。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失