再纯化

A complex sample containing BMP and sodium fluoride was implanted under the abdominal skin of young male Wistar rats. The histiological characteristcs, and activities of alkine phosphatase and acid phosphatase of the implantan were examined to study the effects of fluoride on biological activities of BMP.

在纯化的BMP中加入氟化物再植入大鼠皮下，通过比较植入物在不同植入时间的组织学变化，碱性磷酸酶、酸性磷酸酶活性及钙含量来反映BMP的生物活性。

The chloroform layer and the aqueous layer were separated by multiple column chromatography including macroporous resin, silica gel and polyamide column and preparative HPLC. The compounds obtained were identified by mass spectra and NMR data.

大青叶乙醇提取物，用氯仿萃取后得到氯仿层和水层，各层再分别采用硅胶柱、大孔树脂柱、聚酰胺柱、制备液相、葡聚糖凝胶柱层析及重结晶等分离手段进行分离、纯化，并应用化学性质和质谱、波谱法（1H-NMR、13C-NMR、HMBC）鉴定其化学结构。

Taste yourself in the enclosed world /Museful Space:To create the best space for musing, it is important to keep the artlessness of the environment to intensify the perception and purify one's mind.

触感隔绝/光、风 museful space：冥想空间之围塑：旨在环境之单纯化，强化感官之接触面，净下人的思考，透过光与风这两种媒介，隔离再恣强化，划分出个人的冥想空间。

Then, catechin monomer was isolated and purified by HSCCC with the purity above 98%.

再以该茶多酚为原料，应用HSCCC分离纯化儿茶素单体，所得EGCG产品的纯度达到98%以上。

And Catt in combination with poly-aerylamide gel preparative electrophoresis to isolate serum high density lipoprotein in a pure form was developed.

作者以多聚阴离子—硫酸右旋糖酐沉淀分离，再以聚丙烯酰胺凝胶制备电泳进一步纯化，可获得较纯净的血清高密度脂蛋白。

Also the molecular structure and functional effects of this active component were studied in detail. 1 Extraction, separation, purification and structural identification of the functional component in Chufa After initial handling of waste slurry to solid powder, ethanol was used to lixiviate it followed by the extraction with petroleum ether,ethyl acetate,n-butyl alcohol one by one, the polarity of three organic reagents being changed from low to high.

荸荠功能因子的提取、分离、纯化及结构鉴定以马蹄粉厂产粉废浆为原料，经过实验室初处理成固态原料粉后，用乙醇浸提，再将浸提物以水悬浮，依次通过极性从小到大的石油醚、乙酸乙酯、正丁醇三种有机溶剂两相萃取分部，并以金黄葡萄球菌、枯草芽孢杆菌和致病大肠杆菌为指示菌，检测上述各部分提取物的功能效果。

Adenosine was used as the raw material, dissolved in triethyl phosphate and then esterified with thiophosphoryl chloride under 0~5℃ for 8h without water and oxgen, in alkaline condition provided by 2,6-dimethylpyridine, introducing thiophosphoryl ester to adenosine.Then superfluous triethylamine was added and reacted under 5℃ for 1h to synthesize rough 5'-AMPS.The unpurified product was appled to a DEAE Sephadex A-25 column and eluted with triethylammonium bicarbonate.

用腺苷为起始原料，溶解于磷酸三乙脂中后，在无水无氧环境中及2，6-二甲基吡啶提供的碱性条件下与三氯硫磷以0~5℃下发生酯化反应8h，将硫代磷酸酯引入腺苷，然后再与过量三乙胺在5℃下反应1h，合成粗产品，再用碳酸氢三乙胺作洗脱液，过DEAE Sephadex A-25凝胶柱纯化。

The invention uses the fermentation liquor of one Escherichia coli generating polysialic acid as material, filters and removes thallus, adds some solvents as sodium chloride and alcohol into supernatant to deposit polysialic acid, dissolves the polysialic acid deposits via deionized water, filters to remove modified protein, uses alkali prolease to treat filtrate, complexes deposits via cetyl pyridinium chloride or cetyltrimethylammonium bromide, and dissociates, uses over alcohol to deposit polysialic acid, dissolves the deposits via ultra-pure water, grades and purifies via chromatography column, collects and dialyzes eluent, cools and dries to obtain polysialic acid.

本发明以一株产聚唾液酸大肠杆菌的发酵液为原料，经过过滤去除菌体，上清液加入适量氯化钠和乙醇等溶剂沉淀出聚唾液酸，聚唾液酸沉淀用去离子水溶解，再用过滤去除变性蛋白质，滤液经过碱性蛋白酶处理、氯代十六烷基吡啶或十六烷基三甲基溴化铵络合沉淀并解离后，再用过量乙醇沉淀出聚唾液酸，聚唾液酸沉淀用超纯水溶解，用层析柱进行分级纯化，收集洗脱液经过透析，冷冻干燥得聚唾液酸产品。

BMSCs were isolated, depurated, cultivanted, and identified,then incubated with the concentration of 25μg Fe per milliliter at 37℃in 5% CO2. The labeled cells were stained by Prussian blue/trypan blue,and observed under fluorescent microscope.2. The labeled cells of different density (1×104/ml,5×104/ml,1×105/ml,5×105/ml,1×106 /ml,5×106/ml)were imaged by MRI with T1WI, T2WI and T2*WI sequences;and the same density (5×104/ml,1×105/ml)labeled cells were imaged by T2*WI sequences at different time.Then the signal intensities were measured and statistically analyzed.3. The model of rabbit renal ischemia-reperfusion injury was set up and treated. Then BMSCs(5×105)were injected into 16 recipient rabbits(1abeled cells in 10,unlabeled cells in 6)from ear vein.MR images of kidneys were obtained respectively at the time points of 0,1,3,5, 8 days after transplantation and before transplantation. MR imaging findings were analyzed,which were correlated with histological findings.

实验方法1分离、纯化、培养、鉴定兔BMSCs并以SPIO以25μg Fe/ml培养液浓度标记，对标记后不同时间的细胞行普鲁士蓝染色和台盼蓝拒染后显微镜观察。2将不同细胞浓度标记细胞管(1×104/ml、5×104/ml、1×105/ml、5×105/ml、1×106/ml、5×106/ml)，以不同扫描序列T1WI，T2WI，T2*WI(GRE进行MR成像，再选择相同细胞浓度组(5×104/ml、1×105/ml)进行不同时相MR成像，并测量信号强度，进行统计学分析。3缺血再灌注肾损伤模型建立和处理，然后将标记和未标记细胞(5×105个)经耳缘静脉移植入家兔体内(共16只：注入标记细胞者10只，注入未标记细胞者6只)，两组均于注射前、注射后第0、1、3、5、8天应用MRI对移植细胞进行活体示踪并与肾脏组织切片对照，然后对收集的信号强度进行统计学分析。

Methods: One-step trizol had used to get the total RNA of the four animal brain, then PEGS000 to purify the small RNA from the total RNA, after the ligation ofpoly on the 3\' and the adaptor on the 5\', the oligo primer will be used to reverse translation the small RNA, and then used the specific primers to amplification the small RNA sequences. After the gel purition recovery, the small RNA will be ligated to the T-vector, and be transformed to the TOP 10 E. coli, at last, we picked up the masc. clones for sequencing.

通过Trizol一步法提取四种动物脑中的总RNA，接着用PEG纯化其中的小分子RNA，再经过poly连尾反应在小RNA的3'端连接上一段poly，然后在5'端连上adaptor，用Oligoprimer将连接好的小RNA进行反转录，用根据adaptor和Oligoprimer设计的引物来扩增小RNA，扩增后的小RNA进行胶回收后连接到T载体上，转化TOP10大肠杆菌后挑取阳性克隆进行测序，得到的序列再经过生物信息学的分析后确定是否为miRNA。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失