再确认

If you want to make a return journey, please revalidate your octopus card or buy a new single-ride ticket.

如需要回程之乘客，请再重新确认八达通卡或购买单程票。

If you want to make a return journey , please revalidate your octopus card or buy a new single-ride ticket.

如需要回程乘客，请再购买单程票或重新确认八达通卡啦，多谢你使用轻铁服务。

If you want to make a return journey, please revalidate your octopus card or buy a new single-ride ticket.

如需要回程之乘客，请再重新确认八达通卡或购买单程票，多谢你使用轻铁服务。

If you want to make return journey, please buy a new single ride ticket or revalidate of your octopus card.

如需要回程麰愤，请再购买单程票或重新确认八达通卡啦，多谢你使用轻铁服务

If you want to make a return journey, please buy a new single ride ticket or revalidate of your octopus card.

如需要回程麰愤，请再购买单程票或重新确认八达通卡啦，多谢你使用轻铁服务。

If you want to make a return journey, please buy a new single ride ticket or revalidate your octopus card.

如需要回程麰愤，请再购买单程票或重新确认八达通卡啦，多谢你使用轻铁服务。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

At present, as per usual, but I'll clarify that with you at a later time, Lieutenant Compton.

跟平常一样，我待会再跟你确认康普顿中尉

It is just the same as glue one plaster to your month, if you want to talk, after making sure that your content conforms to the requirement then pull over the plaster.

这个就像每个人的嘴上都贴了一层胶布，你想说话，确认你内容完全符合要求后我才把胶布撕开让你说，说错了，我再掌嘴。

The " that the customer that thing buys on the net wants to click a website to offer only is fictitious adorn mode ", its are relevant the video information Jiang of body system part instantly You is high-definition photograph absorb like the head obtain, had connected high speed network to deliver website server in time next, had connected technology of confluence of video He three-dimensional model and high speed apply colours to a drawing technology, the three-dimensional view effect of the consumer that Jiang has appareled feedbacks quickly on Zai screen, consumer should have told mouse pulling Dong only, with respect to the result that can confirm each Ge trend, deciding again finally is to deny click buy.

网上购物的消费者只要点击网站提供的&虚拟佩戴模式&，其相关身体部分的视频信息将立即由高清摄像头摄取获得，然后通过高速网络及时地传递到网站服务器，并通过视频和三维模型融合技术及高速渲染技术，将穿戴好的消费者的三维视图效果迅速反馈在屏幕上，消费者只要通过拖动鼠标，就能确认各个方位的效果，最后再决定是否点击购买。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。