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再生

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The results indicated:0.1 mg/L 2,4-D or 0.1 mg/L 2,4-D+0.2 mg/L KT was optimal to induct the callus of carrot hypocotyls.The majority of the callus induced on the medium containing 0.1 mg/L 2,4-D regenerated embryoid,whereas,the callus induced on the medium containing 0.1 mg/L (2,4-D)+0.2 mg/L KT produced adventive buds.During the growth of regenerated plant,MS medium is available for rooting of seedling,and seedling regenerated on B5 medium must be placed on the B5 medium containing 0.1% IBA to develop root.

试验结果表明:0 1mg/L2,4 D或0 1mg/L2,4 D+0 2mg/LKT的激素组合有利于胡萝卜下胚轴的愈伤组织诱导;在0 1mg/L2,4 D的培养基上形成的愈伤组织主要以胚状体的形式再生,而在含0 1mg/L2,4 D+0 2mg/LKT激素组合的培养基上形成的愈伤组织主要以发生不定芽的方式再生再生过程中,B5基本培养基上的苗子不易生根,需要附加0 1mg/L的IBA,而在MS培养基上苗子可直接形成根。

The plant expression vectors were constructed and two genes were transferred into A. thaliana and N. tabacum, antikanamycin plants were obtained.4. The trials of adventive bud regeneration directly and callus induction was conducted to select out the optimal explants and formula. The results revealed that double scale can produce 13~16 adventive buds per centimeter in MS medium added 10.0 mg/L 6-BA, 1.0 mg/ L NAA, 40 g/L sugar and 7.5 g/L agar. Scale, leaves, scape and different tissue of flower can be induced to produce callus in appropriate medium, but the filament with anther was the optimized explant.

构建了两个基因的pBI121和pCAMBIA1300植物表达载体,并通过农杆菌将上述基因转入烟草和拟南芥中,得到了卡那抗性的再生植株。4、以中国水仙不同部位外植体进行了不定芽直接分化和愈伤组织诱导分化试验,结果表明,双鳞片外植体在MS0+6-BA 10.0 mg/L+NAA 1.0 mg/L+蔗糖40 g/L+琼脂7.5 g/L培养基上可以大量地直接再生再生芽数量平均可达13~16个/cm双鳞片,比传统的鳞茎盘之间分化提高30~50%;鳞片、叶片、花葶及花部不同组织在适宜的配方中均可不同程度地诱导出愈伤组织,但以带花药的花丝诱导出的愈伤组织最好。

We got the result: Bufo Raddei Strauch Tadpole (1) Bufo Raddei Strauch Tadpole was capable of lens regeneration, which originated from the epithelial cells at iris dorsal margin by dipigmentation.(2) Dipigmentation of Bufo Raddei Strauch by two methods: one was licked up by amoebocyte, the other one was flowed from the pigment cells.(3) In the lens regeneration cells of Bufo Raddei Strauch profiles of the mitochondria became complicated ribosome manifold, and the endoplasmic reticulum was cisternal dilation. During the process of deferentiation of lens fiber the cell nucleolus became little and chromatin shrinked.(4)The crystalline electrophoresis of Bufo Raddei Strauch Tadpole lens regeneration showed that during lens regeneration P-crystalline was expressed earliest then was γ-crystalline and α-crystallin, this result is consistent with newt.

结果表明:花背蟾蜍蝌蚪(1)具有晶状体再生的能力,其晶状体的再生来源于虹膜背缘的上皮细胞;(2)虹膜背缘去色素是转分化的前提,主要有两种方式:一种也是最主要的方式是在晶状体被摘除后虹膜外侧产生巨噬细胞,巨噬细胞吞噬虹膜色素上皮细胞中的色素颗粒;另一种方式是虹膜色素上皮细胞自身释放色素颗粒;(3)电镜观察发现虹膜色素上皮细胞转分化过程中线粒体有明显变化,核糖体增多,粗面内质网增多,晶状体纤维分化过程中细胞核逐渐凝聚;(4)SDS-PAGE电泳发现,花背蟾蜍蝌蚪晶状体再生中相关蛋白表达顺序与有尾类蝾螈相似,蛋白表达顺序依次是β-晶状体蛋白、γ-晶状体蛋白和α-晶状体蛋白,并且后两种蛋白几乎同时表达。

The experiment results of different recycling agent showed that the peptization of recycling agent developed in this paper for the asphaltene is better, and the advantage of this recycling agent was proved from the point of view of asphalt colloid structure.

对不同再生剂的试验结果表明本文开发的再生剂对沥青质的胶溶能力较好,从沥青本质的胶体结构角度证明了该再生剂的优越性能。

BACKGROUND: The augmenter of liver regeneration is a kind of important secondary liver regeneration factors, and it can particularity promote the liver regeneration.

背景:肝再生增强因子是一种重要的次级肝再生因子,能够特异性地促进肝脏再生

Fourth, the membrane recovering method was found within stationary tubular membrane elements and was compared with rotating flow by using experiment, that is, the unloading pressure recovering method is one of the most efficient method, it may reduce the auxiliary time, such as washing , backflush, reverse filtration, but the tubular membrane is washed or backflush or changed after the cycle period are accumulated numerous times.

第四、对旋转流静止管式膜的再生进行了实验比较,并提出了旋转流膜管可以通过卸压再生,以减少膜管每次使用后的冲洗、反吹等辅助时间,采用该种方法再生的膜管其渗透通量完全能满足要求,当循环使用一定次数后再进行或冲洗或反吹或更换新膜管。

According to the theoretical analysis,the results show that the fresh air dehumidified before mixed with return air is more energy-saving among many kinds of realization shapes.Indoor exhaust used as regeneration air can also reduce the total energy consumption of the system.It may enhance the regeneration property when the recuperator is adopted to absorb energy to preheat the dilute liquid.

对此空调系统进行理论分析,得出如下结论:在溶液除湿蒸发冷却空调系统的多种实现形式中,新风经除湿后与回风混合比新回风混合后再除湿节能;将室内排风返回溶液再生器,进行热湿交换,既可加强对处理空气的预冷,回收排风冷量,又可提高再生器的效率,降低系统能耗;把回热器吸收除湿浓溶液中的部分热量用于溶液再生器的稀溶液预热,可以提高溶液的再生性能。

For instance, skin, the largest organ of human body, can be regenerated and recuperated in situ with the same structure and function with that of original skin in case of injury or ulcer by in situ regenerative therapy of human organs.

人类再生生命科学是研究探索人体的再生潜能并将其再生潜能用于治疗人体疾病、保障人体健康、延长人体寿命的生命科学新领域,现在已建立了人体各器官原位再生复原的应用技术,并成功的用于临床多年。

To improve spinosyn-producing strain and enhance spinosyns yield, we studied the effects of glycin concentration and the operational time, temperature and lysozyme concentration on protoplast preparation of Saccharopolyspora spinosa SP06081. We also studied different regeneration media and osmotic stabilizing agents.

为了改良多杀菌素生产菌种,提高多杀菌素产量,研究了甘氨酸添加浓度、溶菌酶作用时间、温度和浓度对多杀菌素生产菌刺糖多胞菌Saccharopolyspora spinosa SP06081菌株原生质体制备和再生的影响,并考察了不同再生培养基和渗透压稳定剂对其再生的影响,确定了该菌株原生质体制备和再生的最佳条件。

According to the working principle and process of regenerative injection of the regenerative liquid propellant auxiliary chamber gun, this thesis analysis the process of regenerative injection cycle and puts forward the structure of the auxiliary chamber.

本文从再生式液体发射药辅助药室火炮的再生喷射原理和工作过程出发,分析了辅助药室火炮再生喷射循环过程,提出了再生式液体发射药辅助药室的结构。

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In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

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