再溶解

PAP-C23, amplified from mature pokeweed antiviral protein gene by PCR, was cloned into the expression vector PET101, and then the recombinant plasmid was transformed into Escherichia coli BL21(DE3). The recombinant mutant PAP was expressed high-levelly induced with IPTG.

用KCL溶液染SDS-PAGE胶，切下表达的目的蛋白，冰浴研磨后用磷酸缓冲液溶解，再加入等体积的福氏不完全佐剂，乳化完全后，免疫家兔，制备抗PAP蛋白的多克隆抗体，间接ELISA法测定所制备的抗血清的效价为1：1000。

At an electrode potential of +0.87 V, the electrode surface was rich of acicular Sn and stannum oxide. This film would prevent the electrode from dezincification, and the current density decreased and homeostasis was reached at the same time. The corrosion mechanism of brass welded line in artificial seawater was dissolution/redeposition.

随后，电极表面积累的锡和锡的氧化物及腐蚀产物层对脱锌腐蚀有一定的阻碍作用，电流密度有所下降，最后腐蚀达到一种动态平衡，腐蚀机制为溶解－再沉积机理。

The inclusion bodies were solubilized in 50mM Tris-buffer at pH 12.5 and then were diluted to refold the denatured proteins. Refolding of D-hydantoinase by direct and fed-batch operations was investigated . Furthermore, the influence of the additives, CTAB and MnCl2, for soluble protein recovery was also studied.

实验中先以pH 12.5， 50mM Tris-bufferru将包涵体溶解，再藉由稀释法使其复性，并探讨直接稀释法与馈料稀释法对乙内醯尿酶复性的影响，另外探讨添加剂是否对可溶蛋白回收率有所帮助。

The same is carried out in the microwave, ethanol, dichloroethane, sodium carbonate, tetrabutylammonium iodide ammonium dissolved in dimethylformamide, then access to carbon dioxide.

同样是在微波炉中进行，把乙醇，氯乙烷，碳酸钠，四丁基碘化铵溶解在二甲基甲酰胺中，再通入二氧化碳。

The sediment of sulfide can be separated from the solution by filtration and syphon. After the sediment is dissolved by diluted nitric acid, Ni 2+ and Co 2+ in solution can be determined by the flame atomic absorption spectrometry method.

将沉淀过滤，或者加入明矾以加速硫化物的沉降、凝结，再用虹吸法吸去上层清液的方法将沉淀分离，用热稀硝酸将沉淀溶解后直接用火焰原子吸收法测定镍、钴的含量。

Microstructure evolution in eutectoid steel during warm deformation in the temperature range from 600 to 700℃ was investigated by simulating hot compression test, SEM and thermomagnetic measurement. The effects of deformation and subsequent annealing on the spheroidizing of pearlite, refining of microstructure and dissolution of cementite were studied.

利用热模拟压缩变形、SEM和热磁法实验，研究了共析钢在600~700℃变形过程的组织演变规律，探讨了变形及随后保温过程对珠光体球化、组织超细化和渗碳体溶解、再析出的影响。

Experience the rest days or by a change from deep color ink color ink printed tyvus shall be on the roll for cleaning, washing solution is: water roll into channels with petrol pouring water roller brushes with large, uniform brushlelss chemiluminscent of monlchamus and the unclean is gasoline fully dissolved, and then re-use, steels side brush, if you are still not enough clean water to flow down available sheet steel threader chemiluminscent zoysiagrass strickle wash in the direction of several times that if the water froze chemiluminscent set of dirty substitute a new set of boottopping.

遇到休息日或者由由深颜色墨改印浅淡颜色墨时，须对水辊进行清洗，清洗的办法为：将水辊放入水槽内，先用汽油浇注水辊，并用大毛刷均匀刷，使水绒套上的墨污被汽油充分地溶解，然后再用清水冲洗，边冲边刷，如果仍不够干净可用钢片顺着水绒套丝缕的方向刮洗几遍，假若水绒套脏得厉害，可更换新的水线套。

The whole column is placed in a thermostatically controlled heating jacket. A volatile sample is introduced into the column using a syringe, and an unreactive carrier gas, such as nitrogen, passed through it. The components of the sample will be carried along in this mobile phase. However, some of the components will cling more readily to the stationary phase than others, either because they become attached to the solid surface or because they dissolve in the liquid.

将整根柱子固定在一个可控制的恒温热罩内，用注射器把易挥发的待分析样品加入到柱子中，再令反应惰性的气体载体通过柱子，例如氮气，此时样品的各成份就会在流动相中移动，然而其中某些成份更倾向依附于固定相，可能的原因是它们能够依附于固体表面或是溶解于固体表面的液体中。

FOUR STAGES OF POST-OLYMPIC URBANIZATION: I. Dissolve / II. Urbanize / III. Disperse / IV.

Re-Fabricate 后奥林匹克都市化的四个阶段：溶解/都市化/分散再织

Taking advantage of better solubility and thermal stability of ionic liquid, the ionic liquid supported acetylacetone catalyst can be simply recycled by water washing or vacuumdistillation.The background of this research is renovation on the traditional oxidation reactions by green chemistry principle.

根据离子液体支载催化剂的两个特性：在水中良好的溶解性和具有很好的热稳定性，在反应结束后通过水洗涤或精馏的方法，很方便的对催化剂进行了回收再利用。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。