再混合

Primary culture of goat mammary gland cells could be derived by outgrowth of migrating cells from fragment of tissue. Fibroblast and epithelial cells could be pured according to their different sensibility to trypsin. Complex cultures treated by 0.25% trypsin in Hanks' for 5~7min at 37℃, the dispersed cells were mainly fibroblasts,then the rest treated by 0.15% trypsin-0.02% EDTA in Hanks'for 5~8min at 37℃, the majority cells harvested were mammary epithelial cells.

用组织块培养法可获得良好的山羊乳腺细胞原代培养物；培养的山羊乳腺成纤维细胞比上皮细胞对胰蛋白酶更敏感，据此可在传代过程中将二者分离纯化，获得纯细胞系；混合培养物先用0.25%胰蛋白酶在37℃消化5~7min 所收集的细胞主要为成纤维细胞；再加0.15%胰蛋白酶-0.02%EDTA 消化液在37℃继续消化5~6min 所回收的细胞绝大多数为上皮细胞，经过2~3 代，即可得到纯化的乳腺上皮细胞系。

The best way to prepare Guizhi Fuling Pills Decoction is as follows,first, extracting Herbal pieces With volatile components(Ramulus Cinnamomi,Cortex Moutan, Radix Paeoniae Rubra and Semen Persicae)by SFE-CO2,then mix dregs with other medicinal materials,and extracted by SBE.

桂枝茯苓丸方药提取，以先将含挥发性成分的中药(桂枝、牡丹皮、赤芍、桃仁)用SFE-CO2萃取，其药渣与方中剩余的茯苓混合，再用SBE法提取为佳。

The plant's treatment train includes chemical clarification, recarbonation, multimedia filtration, granular activated carbon, reverse osmosis, chlorination and blending.

21水厂的处理工艺流程包括：化学净化，再碳酸化，多媒介过滤，活性碳处理，反渗透，氯处理和混合。

In light of Principal-Agent Theory, the dissertation explores the "institution guarantee-government reguarantee " mode under Federal Family Education Loan Program and the Indonesian mode of "institution administration-mixed guarantee", analyzes the essential factors affecting the success of case programs and proposes the core contents of GSSL "higher education institutions-government guarantee" mechanism, which are: By referring to the GSSL coverage, loan amount and default rate in various higher education institutions, the government decides the proportion of taking default loan and shares with the institutions the collected default so as to motivate them to manage the GSSL in accordance with its goal of financial aid.

本文运用委托—代理理论，剖析美国联邦家庭教育贷款&机构担保—政府再担保&模式和印度尼西亚助学贷款&高校管理—混合担保&模式，分析案例项目成败的关键因素，提出了国家助学贷款&高校—政府担保&机制的核心内容：政府根据不同高校国家助学贷款的人数获贷率、金额满足度和拖欠率，来确定分担拖欠贷款的比例，并与高校分享追讨回来的拖欠贷款，以此激励高校按照政府的资助目标努力管理国家助学贷款。

The supplement of oxygen,which was used during remineralization, was due to the mix of different water mass in vertical direction, and the gradient variation of nutrients resulted from the remineralization of organic matter in hyposurface and intermediate water mass.

6根据垂向不同水团的表观耗氧量与营养要素比值推断：水团混合为有机物矿化提供氧的补充，同时次表层和中层水团中营养盐垂直梯度变化主要是由于有机质再矿化的结果。

He TiC could reprecipitate again with excess milling and Cu crystal decreasing. TiC-dispersion-strengthened Cu-based composite powders have been prepared from TiC-Cu and Cu-Ti-C mixed powders by MA. The composite powders were prepared into composite materials by pressing, sintering and heat-extrusion.

IC混合粉末经机械球磨后，再经压制、烧结和热挤压制备成 n 弥散强化铜基复合材料。

Using New Zealand rabbit ears cartilage makes chondrocyte suspension, Fibrinogen was mixed with D-hank's makes a final fibrinogen concentration of 50mg/ml mixture solution, then chondrocytes resuspend with fibrinogen solution, chondrocytes-seeded fibrinogen was mixed with thrombin (25U/ml in 40mM calcium choride) to make chondrocytes/fibrin glue polymer. Pluronic F-127 was mixed with D-hank's makes a final Pluronic F-127 concentration of 400mg/ml mixture solution, then chondrocytes resuspend with Pluronic F-127 solution to make chondrocytes/Pluronic F-127 polymer. The chondrocyte concentrations was 10 million chondrocyte/ml of polymer.

为确定纤维蛋白凝胶与Pluronic F-127在注射方式形成组织工程化软骨过程中的优劣，我们进行了如下实验：应用新西兰大白兔耳软骨获取软骨细胞并体外培养，纤维蛋白原应用D-hank's液配制为50mg/ml，然后以纤维蛋白原溶液重悬软骨细胞，再与40mM的氯化钙配制的25U/ml凝血酶混合形成软骨细胞—纤维蛋白凝胶复合物；Pluronic F-127应用D-hank's液配制为400mg/ml，同样用PluronicF-127溶液重悬软骨细胞而形成软骨细胞—Pluronic F-127凝胶复合物。

In shelf seas,turbulent mixing is central tomany physical and biological processes,as it is often the key determinant of watercolumn structure and nutrient fluxes,and hence the rate of primary production,and also the settling,resuspension,aggregation and disaggregation of particulatematter.

在陆架海中，湍流混合是众多物理、生化过程的核心，它是水体结构及营养盐通量的关键控制因子，并控制着初级生产率及颗粒物的沉降、再悬浮、絮凝与解凝过程。

3 Reweigh, dilute to volume, stopper, then mix by inverting the flask several times.

再称量一次，稀释至体积，盖上塞子，翻转容量瓶数次混合溶液。

Sporophores of Rhizopogon luteolus were collected, air-dried and crushed into a fine powder.

再将放射松的种子与孢子果的细粉加水混合，则每粒种子约含1.9 × 106个孢子。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐