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Methods Activate groups A and C meningococcal polysaccharide with cyanogens bromide and prepare into MenAPS-ADH and MenCPS-ADH derivatives by reacting with 1, 6-adipic acid dihydrazine. Mix MenAPS-ADH and MenCPS-ADH derivatives with purified tetanus toxoid at a certain mass ratio, then add l-ethyl-3(3-dimethylaminopropyl)-carbodiimide to prepare bulks of MenAPS-TT and MenCPS-TT conjugates respectively. Mix the two bulks at a certain ratio, add stabilizer and lyophilize to prepare group A+C meningococcal polysaccharide conjugate vaccine.

A群和C群脑膜炎球菌多糖在溴化氰的活化下,以1,6己二酰肼作为连接子,与精制破伤风类毒素在碳二亚胺作用下结合,再按一定的比例将两者混合,制成A+C群脑膜炎球菌多糖结合疫苗原液,加保护剂冻干后,制成A+C群脑膜炎球菌多糖结合疫苗。

While the methoxide is blending, he turns back to the oil.

虽然是甲醇混合,再回头向石油。

In this paper, adopted augmenting culture in nitrite bacteria culture media and the method of silica gel plate isolation,31 strains of bacteria were isolated from vegetable garden soil of our school. The color reaction test was carried out with Griess reagent and culture fluid, which was regarded as the index to determine producing NO2- or not. 13 strains which color were deep were obtained and they were further rescreened by doing nitrite test. A strain N4(coded N4,the same to the following)with higher rate of nitrosification was picked up after rescreened; A strain B08( coded B08 ,the same to the following) with higher rate of denitrification was obtained after isolated and rejuvenated from our lab conserving mixed denitrifying bacteria culture.

本研究采用亚硝化细菌富集培养基选择培养和硅胶平板分离法,从本校农场菜园土中分离到31株细菌,以格利斯试剂对培养液的反应颜色深浅作为指标衡量其产NO_2~-的多少,经初筛从分离株中筛选出13株格利斯试剂反应颜色较深的菌株,再对这13株菌作亚硝化试验,最终选出一株亚硝化速率较高的菌株(编号为N_4,下同);另外,通过对本实验室保存的反硝化细菌混合菌液进行分离复壮,筛选出一株反硝化速率高的菌株编号为B_

All of those confirmed the odontogenetic ability of adipose-derived stem cells, offered theory and experimental basis for the abundant seed cells in tooth tissue engineering, construct new research model for the micro-environment of the odontogenesis in vitro and vivo.

目的 本研究通过酶消化法分离培养脂肪干细胞,运用牙胚细胞条件培养液体外诱导脂肪干细胞向成牙本质样细胞分化,再将脂肪干细胞与成釉器上皮细胞混合接种入体内建立牙髓-牙本质复合体模型,证实大鼠脂肪干细胞的成牙潜能,为牙齿组织工程化研究获得大量、稳定、可靠的种子细胞提供理论和实验依据;并为体内、外成牙微环境的机理研究构建新的模型。

The aim of shuffling the pais is to mix them thoroughly so that no one can recognize them anymore.

洗牌的目的是要将牌混合透切而令到无人可以再作辨认。

From calcium law to sodium law again to more advanced mix alkaline process and low temperature sodium law, not only from enhances the pentaerythritol the production rate aspect to begin, is similarly important to the environment pollution as well as to person's injury degree.

从钙法到钠法再到较先进的混合碱法和低温钠法,不仅从提高季戊四醇的产率方面着手,对环境的污染以及对人的伤害程度同样重要。

Of Piso all must think and say that his character is an excellent blend of firmness and gentleness, and that it would be hard to find anyone possessing a stronger love of leisure, or, on the other hand, more capable of action, and of taking the necessary measures without thrusting his activity upon our notice.

世人评价皮索大人说他的性格是坚定与优雅的绝妙混合,很难再找出一个人能比他更强烈的热爱文娱或是更有才干的了,能在不惹众人注意之下采取必要的行动。

Reparation of PBO was begun with the polymerization process in which composite salts that contained 4,6-diaminoresorcinol and terephthalic acid were dissolved in polyphosphoric acid and phosphorus pentoxide.

BO纤维制备:以4,6-二氨基间苯二酚的盐酸盐为单体原料,与对苯二甲酸混合,经过中和反应、脱色处理、干燥后制成复合盐;再以多聚磷酸为溶剂,添加五氧化二磷和抗氧化剂,经过预聚合反应制备出PBO聚合物;聚合物通过双螺杆挤出机进一步进行聚合,然后通过喷丝板挤出,在磷酸水溶液中凝固成型,经过洗涤、干燥等过程,最终卷绕成型。

METHODS: 15 g/L silk fibroin solution was made by degumming, salvation and dialysis. Chitosan was dissolved in 2% acetic acid solution to prepare 25 g/L chitosan-acetic acid solution. Two solutions were mixed to prepare six silk fibroin/chitosan solutions, and mass ratio was 10: 0, 5: 5, 4: 6, 3: 7, 2: 8, 0: 10. These solutions were separately sucked in a 24-well plate. Following exhausting gas vacuole at 4 ℃, precooling was performed at -20 ℃ for 12 hours, followed by cryodesiccate for 30 hours.

通过脱胶、溶解、透析这3个主要步骤制成质量浓度为15 g/L的丝素溶液,将壳聚糖溶解在2%的醋酸溶液中制成25 g/L的壳聚糖-乙酸溶液,然后将两者混合,配制成丝素/壳聚糖质量比分别为10∶0,5∶5,4∶6,3∶7,2∶8,0∶10的6种溶液,分别吸入24孔板中,4 ℃排出气泡后,-20 ℃预冷冻12 h,再冷冻干燥30 h。

Primary culture of goat mammary gland cells could be derived by outgrowth of migrating cells from fragment of tissue. Fibroblast and epithelial cells could be pured according to their different sensibility to trypsin. Complex cultures treated by 0.25% trypsin in Hanks' for 5~7min at 37℃, the dispersed cells were mainly fibroblasts,then the rest treated by 0.15% trypsin-0.02% EDTA in Hanks'for 5~8min at 37℃, the majority cells harvested were mammary epithelial cells.

用组织块培养法可获得良好的山羊乳腺细胞原代培养物;培养的山羊乳腺成纤维细胞比上皮细胞对胰蛋白酶更敏感,据此可在传代过程中将二者分离纯化,获得纯细胞系;混合培养物先用0.25%胰蛋白酶在37℃消化5~7min 所收集的细胞主要为成纤维细胞;再加0.15%胰蛋白酶-0.02鞹A 消化液在37℃继续消化5~6min 所回收的细胞绝大多数为上皮细胞,经过2~3 代,即可得到纯化的乳腺上皮细胞系。

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It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐

The eˉtiology of hemospermia is complicate,but almost of hemospermia are benign.

血精的原因很,以良性病变为主。