再沉淀

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

In solvent and under the action of certain catalyst, anilino methyl siloxane is reacted to obtain solid cage anilino methyl sesquisiloxane octamer acid salt; and through filtering, washing, precipitation and reaction with amine desalting agent, cage anilino methyl sesquisiloxane octamer is finally prepared.

本专利提供了一种笼型八聚苯胺甲基倍半硅氧烷的制备方法，苯胺甲基硅氧烷在一定溶剂和催化剂作用下反应，过滤得到笼型八聚苯胺甲基倍半硅氧烷酸式盐固体，经过洗涤、沉淀，再与胺类脱盐剂作用，最终制得笼型八聚苯胺甲基倍半硅氧烷。

Through a variety of resin filter, adsorption and elution conditions for the optimization, as well as decolorization, crystallization methods of research, this article eventually determine the strong acid cation resin HZ-001 which is at pH 3, 3BV / h, having the largest adsorption to the tryptophan; the fermentation broth were centrifuged to remove sediment,then treated with activated carbon to be decolored and then treated with strong acid cation resin HZ-001. After that,2mol/l amino acid were passed through the HZ-001 ion exchanging column,kept almost constants when the flow rate stayed 6 BV / h. At 60℃,it was condensed into the saturated state and then added Ethanol until its percentage reaching 30%. At 4 ℃ under 100rpm placed in the shaker 20h, the collection of L-tryptophan was dried up in vacuo.

本文通过对种树脂的筛选、吸附和洗脱条件的优化，以及对脱色、结晶方法的研究与摸索，最终确定强酸型阳离子树脂HZ-001在pH=3，3倍床体积流速，温度室温，对色氨酸有最大的吸附作用；将发酵液离心去沉淀并用活性炭脱色后通过HZ-001强酸型阳离子交换树脂柱，再用2mol/L的氨水6倍床体积流速在室温下进行洗脱，收集洗脱液，60℃浓缩为过饱和状态后加入30%无水乙醇，4℃下100rpm摇起晶20h，过滤搜集结晶并真空干燥得白色粉片状L-色氨酸晶体。

MethodsJirihen Agaru-8 polysaccharide was extracted with water. The herb was deproteined, ethanol precipitated, and purified with dialyses method.

方法吉日很阿嘎如-8经热水抽提，Sevag法脱蛋白，乙醇沉淀，再通过透析得白色粉末状精制多糖。

That's where water treatment comes into the picture. A water treatment plant purifies the water by adding coagulant that precipitate impurities and particles in the water, allowing it to be flocculated and filtered. Chlorine is then added as a disinfectant before it can properly be called "tap water".

滤水厂将收集到的原水净化，一般都是将凝集剂加入原水中，使混杂於水中的悬浮物凝集成较大的粒块，再经过沉淀、过滤的净化过程，最后加入氯气消毒才能成为「滤水」。

Brinkhous of the University of North Carolina at Chapel Hill and Edward Shanbrom of Hyland Laboratories produce a highly concentrated form of Factor VIII by pooling large quantities of plasma that generate vast amounts of cyro, which are then redissolved, treated, filtered, and centrifuged.

Brinkhous博士在查珀希尔和Edward Shanbrom的海兰实验室通过汇集许多能产生大量冷沉淀的血浆制造出富含Ⅷ因子的浓缩物，然后再融解、处理、过滤和离心。

Finally, the selected iron compounds were mixed with skim or whole milk powder directly and stored for a week in room temperature. The fortified milk powder was tested for color, flavor and precipitation after rehydrating by 45°C ion-free distilled water.

最后再将上述经筛选之铁剂以市售铁强化制品之剂量直接与全脂及脱脂乳粉混合并於室温下储存一周后，以45℃去离子水复水，观察其色泽、风味及沉淀与否等项目。

Finally, the selected iron compounds were mixed with skim or whole milk powder directly and stored for a week in room temperature. The fortified milk powder was tested for color, flavor and precipitation after rehydrating by 45°C ion-free distilled water.

最后再將上述经筛选之铁剂以市售铁强化製品之剂量直接与全脂及脱脂乳粉混合並於室温下储存一週后，以45℃去离子水復水，观察其色泽、风味及沉淀与否等项目。

The iron isotopic composition lightly from hydrothermal fluid upwelled into ocean, BIFs are enriched in heavy isotopes of Fe by partial oxidation and precipitation, and iron isotopic composition was homogenous and reequilibrium during different minerals by metamorphism.

提出了地球早期海洋中Fe的循环模式：洋中脊喷发富集Fe轻同位素的二价铁热液流体，流体进入海洋后经过部分氧化和沉淀的过程使得BIF富集Fe的重同位素，后期的变质作用使得不同矿物间发生了Fe的均一化及再平衡

The main reasons were that the algae and organism in the water decreased the ability of removing pollutants by coagulations, the algae which grown again in the open sedimentation tank weakened the removal effect of algae by coagulation; because of the accumulation of algae and algae toxin in the filtration tank, the concentration of algae toxin in the effluent increased; by the function of fluid chlorine, the algae and organisms after the filtration will release microalgae toxin and produce a lot of byproduct such as microalgae toxin, sobomyl and so on, meanwhile, the chlorination produce considerable halide and halo-acetic acid and other disinfection byproduct.

主要表现在藻类和溶解性有机物的存在降低了混凝去除污染物的能力；藻类在露天平流沉淀池中再生长，抵消了混凝除藻效果；藻类和藻毒素在滤池中积累，导致滤后藻毒素升高；穿透滤池的藻类和有机物在液氯作用下，释放微囊藻毒素、土臭素和二甲基异冰片等胞内物质，同时生成数量可观的卤代烃和卤乙酸等消毒副产物。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！