再提取

Abortus, amplify BP26 gene by PCR and clone to pMD18-T simple vector. Identify the constructed recombinant plasmid pMDBP26 by sequencing, then subclone to vector pET-28a. Transform the constructed recombinant plasmid pETBP26 to E. coli BL21(DE3) for expression under induction of IPTG. Purify the expressed product by histidine-binding resin column chromatography and identify by Western blot.

提取布鲁氏菌基因组DNA，用PCR扩增出BP26基因，并克隆到pMD18-T载体上，测序正确后，再亚克隆至pET-28a载体上，转化到大肠杆菌BL21(DE3)中，用IPTG诱导表达，经组氨酸结合树脂柱纯化，并对纯化后的表达产物进行Western blot鉴定。

He cites an experiment at Houston airport where passengers had to walk for one minute from the plane to the baggage reclaim and then wait a further seven minutes to collect their luggage.

他引用休斯敦机场的实验为例。在休斯敦机场，乘客们下飞机之后走到行李提取处得用1分钟，然后再等7分钟才能领到行李。

To fulfill a TV guidance system task of fast recognizing and tracking missile target from sequential images including multiple interferences,a fast segmentation and target characteristic extraction algorithm of sequential images based on binary image index map was proposed.After the sequential images were processed to binary images,a Field Programmable Gate Array was used to scan the binary image once and an index map was built,and then Digital Signal Processing was used to scan the index map once.

针对电视制导系统需从包含多个干扰目标的序列图像中快速识别和跟踪导弹目标的要求，提出了一种基于二值图像索引图的序列图像快速分割及目标特征提取算法，在序列图像二值化后，只需由FPGA对其遍历一次就可得到一张含有目标信息的索引图表，再由DSP对该索引图表边遍历边计算就可得到图像所含目标的数量、面积、质心坐标、二阶矩不变量等特征。

In order to resolve the conflict between excessive data storage, time expensive computation and real-time image processing, a method is proposed based on distance transfer with chamfer to extract the position and scale features of each objects, then a five points square predictor is used to estimate the position of objects next time and compute the velocity. Finally, objects are associated using improved weighed nearest neighbor. The algorithm is implemented on the platform of Xilinx XCV300E+ADI Blackfin 532 DSP.

为了解决多目标跟踪中数据存储量、运算量大与实时处理之间的矛盾，提出了一种基于倒角算子距离变换的多目标分离方法，分别提取出各个目标的位置和大小信息，再采用五点二次预测估计出下一场目标的位置从而得到目标的运动速度，最后由改进的加权最近邻算法进行目标特征关联。

First, extract the natural curve of the dunes and reintegration, and through the rational scrutiny and emotional choices, ultimately form the boundary blurred architectural form in order to meet the design in mind.

首先提取沙丘的自然曲线进行重组，再通过理性的推敲与感性的选择，最终形成边界模糊的建筑形态，以适应设计的初衷。

To this end, the ultrasound cardiograph is filtered and fuzzily boundary enhanced, a threshold is set to obtain a binary image, then the heart's boundary is got through ellipse curve fitting, cubic spline interpretation and smoothing.

首先对超声心动图像进行滤波以消除图像中的噪声，并运用模糊数学理论进行模糊边缘增强，提取超声心动图像的内外膜边缘信息；接著对超声心动图像作二值化处理和椭圆曲线拟合，初步得到心脏的内外膜边缘；再采用相应的三次样条插值和平滑技术，得到光滑、连续的心脏内外膜边缘。

Chlorophyll of mulberry leaves can be extracted by 95% ethyl alcohol and then sodium copper chlorophyllin is made by chemical progress.

以桑叶为原料，95 %乙醇为溶剂提取叶绿素，再经过皂化、铜代、成盐制成叶绿素铜钠盐，产品质量达到国家GB32 36 - 82标

But of this we will take thought hereafter; for the present, let us draw a ship into the sea, and find a crew for her expressly; let us put a hecatomb on board, and let us send Chryseis also; further, let some chief man among us be in command, either Ajax, or Idomeneus, or yourself, son of Peleus, mighty warrior that you are, that we may offer sacrifice and appease the the anger of the god.

我将亲往提取——动怒发火去吧，那位接受我造访的伙计！够了，这些事情我们以后再议。现在，我们必须拨出一条乌黑的海船，拖人闪光的大海，配备足够的桨手，搬上丰盛的祀祭——别忘了那位姑娘，美貌的克鲁塞伊丝。须由一位首领负责解送，或是埃阿斯，或是伊多墨纽斯，或是卓越的俄底修斯也可以是你自己，裴琉斯之子，天底下暴戾的典型以主持牲祭，平息远射手的恨心。

Also the molecular structure and functional effects of this active component were studied in detail. 1 Extraction, separation, purification and structural identification of the functional component in Chufa After initial handling of waste slurry to solid powder, ethanol was used to lixiviate it followed by the extraction with petroleum ether,ethyl acetate,n-butyl alcohol one by one, the polarity of three organic reagents being changed from low to high.

荸荠功能因子的提取、分离、纯化及结构鉴定以马蹄粉厂产粉废浆为原料，经过实验室初处理成固态原料粉后，用乙醇浸提，再将浸提物以水悬浮，依次通过极性从小到大的石油醚、乙酸乙酯、正丁醇三种有机溶剂两相萃取分部，并以金黄葡萄球菌、枯草芽孢杆菌和致病大肠杆菌为指示菌，检测上述各部分提取物的功能效果。

Methods: One-step trizol had used to get the total RNA of the four animal brain, then PEGS000 to purify the small RNA from the total RNA, after the ligation ofpoly on the 3\' and the adaptor on the 5\', the oligo primer will be used to reverse translation the small RNA, and then used the specific primers to amplification the small RNA sequences. After the gel purition recovery, the small RNA will be ligated to the T-vector, and be transformed to the TOP 10 E. coli, at last, we picked up the masc. clones for sequencing.

通过Trizol一步法提取四种动物脑中的总RNA，接着用PEG纯化其中的小分子RNA，再经过poly连尾反应在小RNA的3'端连接上一段poly，然后在5'端连上adaptor，用Oligoprimer将连接好的小RNA进行反转录，用根据adaptor和Oligoprimer设计的引物来扩增小RNA，扩增后的小RNA进行胶回收后连接到T载体上，转化TOP10大肠杆菌后挑取阳性克隆进行测序，得到的序列再经过生物信息学的分析后确定是否为miRNA。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。