再提取

After extraction by ethanol and purification by cationic ion-exchange column, a method of using reversed-phase high-performance liquid chromatography and precolumn derivatization with o-phthalaldehyde and 9-fiuorenylmethylchloroformate is employed for determination of free amino acids in tobacco . This method assures that amino acids with amino and imino groups can be determined simultaneously. The results of duplication experiment and recovery experiment are acceptable.

本试验用乙醇提取烟叶中的游离氨基酸，并用阳离子交换树脂纯化，再用邻苯二甲醛、氯甲酸芴甲酯联合柱前衍生、反相高效液相色谱分析测定了烟叶中的游离氨基酸，该方法能同时测定烟叶中的带氨基和亚氨基基团的氨基酸，并且分析方法的重现性和回收率均令人满意。

Total RNA was extracted from venom cells from Gloydius intermedius, FLE gene was amplified by RT-PCR. Then it was connected to pMD18-T vector by TA cloning. At last, sequencing and analysis the genesequence.

从中介蝮蛇毒腺细胞中提取总RNA，通过RT-PCR法扩增纤溶酶基因，与pMD18-T载体连接进行TA克隆，再进行序列的测定并且对该序列进行分析。

Methods:Total RNA was extracted from venom cells from Gloydius intermedius,FLE gene was amplified by RT-PCR.Then it was connected to pMD18-T vector by TA cloning.At last,sequencing and analysis the gene sequence.

材料及方法：从中介蝮蛇毒腺细胞中提取总RNA，通过RT-PCR法扩增纤溶酶基因，与pMD18-T载体连接进行TA克隆，再进行序列的测定并且对该序列进行分析。

Chromatographic fingerprint index F and chromatographic fingerprint relative index Fr were used as the objective functions for the evaluation,and the extract of Saussurea involucrate by water was used as the sample.

以色谱指纹图谱指数F和色谱指纹图谱相对指数Fr作为评价毛细管电泳分析系统的目标函数，以雪莲药材水提取液为样品，考察一定浓度的硼砂、硼酸、磷酸氢二钠和磷酸二氢钠溶液按三角形优化法和四面体优化法构成背景电解质时对样品的分离情况，通过添加有机改性剂和调节pH进行再优化。

Woodson or apocynum venetum Linn as raw material, it utilizes the method of decoction and alcohol sedimentation to remove impurity. After extract is separated by macroporous adsorptive resins, then use polydextran gel LH-20 to purify, obtain isoquercitrin. Compared with the method of traditional silica gel column chromatography, this method has characteristics such as: experiment period is short, dissipation of organic dissolvent is little, yield of produced product is high, and purity coefficient is fine and so on. Isoquercitrin produced by this invention can be provided for medicine research or used as chemistry control article.

Woodson或罗布麻的叶为原料，采用水提醇沉除杂的方法，提取液经大孔吸附树脂分离后再用葡聚糖凝胶LH-20纯化得罗布麻甲素，此法与传统硅胶柱层析的方法相比，具有实验周期短，有机溶媒消耗少，制得的产品得率高和纯度好的特点，本法制得的罗布麻甲素可供药物研究或化学对照品使用。

Directly reading the 210-layer of 0.65-mm-thick consecutive CT Dicom format images of human body thoracolumbar making use of 0.625-mm isotropous resolution thin layer scanning technique into the Mimics. Defining bone organization threshold, withdrawing each outline, partitioning each edge of layer picture, edited selectively and repaired by hole processing. After a redundancy data was cleaned, the thoracolumbar 3-D finite element geometry model was acquired. Then area mesh could be kept as the document Ansys named .lis as the suffix and be introduced into Ansys software directly to mesh body, and then transferred the body mesh to mimics to assignment son the basis of CT values, once again import Ansys to establish 3-D finite element model.

将以各向同性分辨率0.625 mm薄层扫描所得的层厚0.65 mm人体胸腰段连续断层210层Dicom格式CT图像，直接读入Mimics后界定骨组织阈值、提取各层面轮廓线、图像边缘分割、选择性编辑及补洞处理，去除冗余数据，三维化处理后获得胸腰段三维几何面网格模型，将其保存为后缀名。lis的Ansys文件，直接导入Ansys有限元分析软件进行体网格划分，再将体网格转入Mimics根据CT值给予赋值，再次导入Ansys生成有限元模型。

Jasminum nudiflorum Lindl.1. The fatty acid compositions in Jasminum nudiflorum Lindl.

采用乙醚提取迎春花脂肪酸，再经过甲酯化后，通过气相色谱/质谱联用技术分析脂肪酸的组成。

A frame image of a video frequency sequence is read into a postpositional memory zone, and staring position and size information of a tracked object is obtained in the frame, then distribution statistical information of a target signature is extracted to build a gauss mixed model as an object template, a mean vector and a covariance matrix in gauss mixed distribution are used to describe the position and the size of the object, then the next frame image of the video frequency sequence is read into the postpositional memory zone. In a new frame video frequency image, a parameter estimation method is used to obtain object gauss mized model parameters in the current frame in iterative computation and find candidate template similar to the object template, and the final model parameters obtained in iteration are used to update the tracking window for realizing self-adaption of the tracked window.

将视频序列的一帧图像读入缓存区，获得该帧中被跟踪目标的初始位置和尺寸信息，然后提取目标特征的分布统计信息建立高斯混合模型作为目标模板，用高斯混合分布的均值向量和协方差矩阵描述目标的位置和尺寸，再将视频序列下一帧图像读入缓存区，在新一帧视频图像中利用参数估计方法迭代计算当前帧中目标高斯混合模型参数找到与目标模板最相似的候选模板，利用迭代得到的最终模型参数更新跟踪窗口实现跟踪窗宽的自适应。

The calligraphic character is firstly skeletonized with morphology fused index table skeletonization method, in which the character is thinned by an index table and then thinned with mathematic morphology method.

首先用融合数学形态学的索引表骨架提取方法获取骨架，即先使用索引表对书法字进行初步细化，再用数学形态学的方法细化得到骨架。

The DNA was extracted from mature Spinacia oleracea L.separately by the method of CTAB, then used as template after mixed by gender to be amplified with the optimized RAPD reaction condition. A total of 160 primers (10 bp) and 40 pairs of primers were screened, 44 primers of which produced clear patterns.

菠菜为雌雄异株植物，用CTAB法提取其雌雄成株幼嫩叶片总DNA分别构建雌、雄株的DNA池，以之为模板，用已优化的RAPD体系扩增，在160条随机引物(10 bp)和40个引物对中筛选出44个扩增效果较好的单引物，再从中进行进一步筛选。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。