再提取

The total RNA from brain tissue of substantia nigra of PD rats is extracted at the 1st, 2nd, 4th and 8th week and comparison is made between it and that from normal rats. The total RNA, after RNA formaldehyde degenerated sepharose electrophoresis and separation and Northern transfer, is adsorbed unto colloxylin membrane. Then ECL labeled Selenoprotein P and an unknown gene fragment are used as probes to hybridize with the RNA on the colloxylin membrane. And the hybridized result is obtained with enzyme-linked immunosorbent assay. Finally, analysis of OD is made using ONE-Dscan software.

分别提取1周、2周、1月、2月的帕金森大鼠（各8只）黑质区脑组织总RNA，并取相对应时间的正常大鼠的脑组织总RNA作为对照，经RNA甲醛变性琼脂糖凝胶电泳分离后，通过Northern转移，将其吸附在硝酸纤维素膜上，然后利用ECL标记的Selenoprotein P和一段未知基因为探针，与硝酸纤维素膜上的RNA分子进行杂交，再通过显影取得杂交的结果，应用ONE-Dscan软件进行光密度值分析。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2+-chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2 -chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

In the studying of image segmentation, six crucial technologies havebeen developed successfully. 1 In one dimension histogram of original DRimage of luggage, there remain maximum-value wave crest havingapproximately stationary position, and the first minimum to the left of thewave crest performs excellently to be used to get rid of background of DRimage. 2 Open operation, one type of math morphology, is the right idea toerase luggage disturbance in DR image and at the same time, can be retaininteresting region appropriately. 3 After open operation, there are only a few minimums on image histogram, one of which can be to segment image furtherand achieves satisfying result. 4 Method of histogram analyzing and that oferosion performs well in the re-segmentation of conglutinative region in DRimage. 5 Method of image distance switch can ensure Luggage CT sectionautomatically obtained to have rich suspicious region information. 6 Todecompose structure element of math morphology is a way to save a great dealrunning time for automatically discerning interesting regions algorithm,apparently improving implementation efficiency.

在对图像可疑区域分割研究中，主要开发成功以下几项关键技术：1发现原始箱包DR图像的一维直方图中，均具有位置相对稳定的最大值波峰，以波峰左边第一个极小值为分割阈值，去除DR图像背景，效果非常好。2运用数学形态学中的开运算能够非常有效地去除箱子的干扰，同时能够比较好地保留可疑区域。3发现开运算后的DR图像直方图只有若干个极小值点的特点，其中用最大值波峰左边的第二个极小值点进一步分割DR图像，能够更有效地提取箱包中的可疑区域。4运用直方图分析法和腐蚀法，对粘连区域实行再分割，效果较好。5运用距离变换法，能确保自动设定的箱包CT截面包含丰富的可疑区域信息。6运用形态学结构元素分解技术，大幅度减少可疑区域的自动判别算法运行时间，提高算法软件执行效率。

This article separated the vocal melody signals from MP3 songs audio signals based on frequency domain convolutive signal blind source algorithm, and then built a song database of humming retrieval system using song melody features extracted from the vocal melody signal.

将通过频域卷积信号盲源分离算法从MP3歌曲音频信号中分离出人声主唱信号，再从人声主唱信号中提取出能够表征歌曲的旋律特征构建哼唱检索系统的歌曲数据库。

The optimum extraction process of preparation was carried out with orthogonal test,and the craft of extraction by the single factor experiment, and I suruey the corrigent to choose suitable quantity. This result would be technology parameters for production of Shuanghuanglian Oral Liquid.

通过正交试验优选出双黄连口服液最佳的提取工艺，再通过单因素试验考察纯化工艺，并对矫味剂进行考察、筛选，从而为大生产提供合理可行的技术参数。

DBP and DEHP have the similar photodegradation mechanism. The predominant pathway for DBP and DEHP photodegradation in the Fe -oxalate complexes system is hydroxyl radical extracting H atom in α-position of the aliphatic chain close to the carboxyl group followed by oxidation to produce alcoholized derivatives ROH. ROH then breaks down to phthalic acid and dialcohol that will be further oxidized up to mineralization.

DBP和DEHP的光降解历程相似，主要反应途径是OH自由基提取烷基上与羧基相连的α-H原子，然后被氧化生成醇衍生物，再分解成为酞酸和二醇，酞酸经历开环和进一步脱羧，二醇可以被氧化成为低级烷基羧酸，直至矿化。

College of Veterinary Medicine, China Agricultural University, Beijing 100094, China Abstract: A method has been developed to determine residual stilbenes such as diethylstilbestrol, dienestrol and hexestrol in animal tissues using solid phase extraction and gas chromatography-mass spectrometry.

动物组织样品在碱性条件下用乙酸乙酯提取，经碳酸钠溶液液-液分配净化，再用硅胶柱固相萃取净化，洗脱液均分成两份后经氮气吹干，分别用双三甲基硅基三氟乙酰氨和七氟丁酸酐衍生，采用选择离子监测模式进行测定，外标法定量。

Meanwhile it was pointed out that with homofermentative LAB, recirculation of cells and in situ production removal technology under anaerobic condition, LA yield could be increased and co-inoculation of various LAB with the optimizing LA separation technique could help to the industrialization of LA production with organic wastes.

指出在厌氧条件下应用同型发酵乳酸细菌，采用原位产物分离技术和细胞再循环发酵通常会提高乳酸的产量；采取多菌种的联合发酵并优化提取工艺，将有助于实现有机废物工业化生产乳酸。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。