再处理

Recently, with profound studies of ischemic preconditioning, protective effect of remote organ preconditioning which was a brief episode of sublethal ischemia in an organ remote from the target organ, was found.

近来，随着缺血预处理研究的深入，发现了远隔器官缺血预处理现象，为采用非生命器官的短暂亚致死性缺血预处理对抗心脑等生命器官的缺血/再灌注损伤提供了线索。

Methods: CD34(superscript +) cells from fetal liver were isolated with a magnetic cell sorting kit and were cultured. Cells pretreated with or without protein kinase C inhibitor chelerythrine chloride (3 μmol/L) were induced by 5 ×10^(-7)mol/L tretinoin for 24 h, and then incubated in serum-free medium. Expressions of genes in treated cells were assayed by Western blotting and RT-PCR.

采用免疫磁珠法分离人胎肝CD34细胞，培养4d后，蛋白激酶C特异抑制剂chelerythrine chloride(3μmol/L)处理24h，再加入维A酸处理24h5×10^(-7mol/L，无血清培养基培养5d，Western印迹和半定量RT-PCR法分析维A酸处理前后神经特异基因表达。

Preparation of the cathode includes:shaping under the press of 40Mpa, sintering at 550℃for 1 hour and at 900℃for 8 hours and threading with molybdenum bar; Considering the literatures we choose CaCl2 as salt for preparation titanium. Pretreatment of salt is for 1 hour at 100℃and for 2 hours at 300℃. Partial pressure of oxygen which need lower than 5.11×10-7Pa to reduct titanium oxides and hygroscopic property of salt need a sealed equipment to electrolyse. And finally successfully designed a satisfied one and the results show that the equipment can be satisfied the requirment of the experiment. Flow of the inert gas is 1.5L/min, the voltage is 2.8 V, temperature is 850℃and time is 2 hours during pre-electrolysis. Flow of the inert gas is 0.2L/min, the voltage is 3.1 V, temperature is 900℃and time changes with the mass of TiO2 during electrolysis, namely the greater need the longer time; To eliminate influence of salt and other impurities, the products need to wash with distilled water and dilute chlorhydric acid , then wash with dilute hydrochloric acid under supersonic wave assistant. Finally, electrometical properties of the electrolysis of TiO2 is researched by cyclic voltammetry and chronoamperometry, and results show that there are two main reodox steps, namely from TiO2 to TiO and from TiO to Ti.

阴极制备主要包括40MPa压力下模压成型、两段式烧结(1小时内升至550℃保温1小时，再1小时升温至900℃保温8小时)及烧结后TiO2块打孔用钼棒串接三个主要环节；实验中选用CaCl2作为电解熔盐，并对其进行预处理(100℃，保温1小时； 300℃，保温2小时)；经热力学计算，还原钛氧化物的氧分压至少要低于5.11×10-7Pa，结合电解过程中所用熔盐CaCl2有极强的吸水性的特点，电解装置应有较高的密封性，自行设计了一套密封性可靠的电解装置，便于实验过程中熔盐预处理和氧分压的控制；通过干燥处理预电解过程中Ar流量大约为1.5L/min、电压为2.8 V、温度为850℃、时间为2小时，电解过程中Ar流量大约为0.2L/min、电压为3.1V、温度为900℃，实验结果表明电解时间与TiO2质量密切相关，质量越大需要电解的时间越长；通过自来水冲洗—稀盐酸浸泡、洗涤—在超声波辅助作用下稀盐酸洗涤，可减少熔盐及其它杂质对电解产物检测结果的影响；最后，通过循环伏安法、计时电流法对电解机理的研究，确定电解还原TiO2制备金属钛主要经历了TiO2-TiO-Ti的过程。

Aim: To investigate the protective effects of adenosine pretreatment and ischemic preconditioning on the contractible function and infarct size in rabbits.

目的：研究缺血预处理与&腺苷预处理&对兔心收缩功能和心肌梗死范围的影响，探讨两种处理方法对再灌注损伤的可能有益作用。

Thus, concentration of 0.10% solution of H2O2 treatment, and then the concentration of 0.0029 "Lu LU Hong God" can serve as a solution to deal with and to break the wild Pennisetum Eragrostis seed dormancy and promote germination and growth of the reagents suitable for soaking approach.

因此，用浓度为0.10%的H2O2溶液处理后，再用浓度为0.0029的&鲁虹神露&溶液处理可以作为打破野生狼尾草和大画眉草种子休眠、促进其萌发和生长的适宜试剂浸种处理方法。

A large drop in hardness appears in as deformed samples at a temperature 45 ℃ lower than the start temperature of the exothermal peak after isochronal annealing, indicating that the recrystallization and grain growth process is closely relatine to annealing time and temperatur.

出现明显晶粒长大的温度较DSC曲线显示的放热峰起始温度低45℃左右，这主要是由于变形组织的回复再结晶过程是退火温度与时间的函数，降低处理温度并延长处理时间能达到与高温短时处理相同的效果。

The plant's treatment train includes chemical clarification, recarbonation, multimedia filtration, granular activated carbon, reverse osmosis, chlorination and blending.

21水厂的处理工艺流程包括：化学净化，再碳酸化，多媒介过滤，活性碳处理，反渗透，氯处理和混合。

The effects of technologic parameters, including concen- tration and temperature of ADP solution and galvanizing time on rising time of anodic oxide films are discussed. 2. After dipped in amino trimethylene phosphonic acid solution, anodic oxide films are annealed in pipe stove.

将化成箔在氨基三甲叉膦酸溶液中浸渍，取出烘干后再进行热处理，研究了 ATMP 溶液浓度、浸渍温度和热处理温度对氧化膜升压时间、比容、耐电压的影响，找到其最佳耐水合处理工艺；采用FT-IR对水合作用后的 ATMP 处理氧化膜和未经耐水合处理氧化膜进行了分析。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

Our method is different to [7], which needs additional end-point detection to cut off cough signal from input signal and then using cough signal to carry out DTW algorithm. In our method, we using sliding window to catch different signal to carry out DTW algorithm, which avoids additional end-point detection. DTW method has computation complexity problem.

有别於相关研究[7]中，需要额外的端点侦测处理，将输入讯号中含有咳嗽声音的部份切割以后，再进行DTW侦测处理，在我们所提出的方法中，利用移动视窗取得不同段落的讯号，进行DTW侦测处理，即可免除额外的端点侦测。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。