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A single donor cell was injected into the perivitelline space of enucleated oocyte and fusion was induced with electric pulses. Reconstructed oocytes were activated using ionomycin (5 M,5min) and 6-dimethylaminopurine (6-DMAP,2mM,5h).

将供核细胞注射到去核卵母细胞的卵周隙,经电融合获得重构胚,再经离子霉素(Ionomycin,5μM,5min)和6-二甲基氨基嘌呤(6-DMAP,2mM,5h)处理激活。

Also, the corrosion performances and electrochemical behaviors of the phosphated/molybdate post-sealed HDG steel sheet in 5% NaCl solution were investigated, using Tafel polarization and electrochemical impedance spectroscopy measurements.

将热镀锌钢板先磷化再用钼酸盐溶液进行封闭后处理,通过塔菲尔极化和电化学阻抗测量研究其在5%NaCl水溶液中的腐蚀电化学行为,并与单磷化的热镀锌钢板进行了对比。

Physalis peruviana L. is a herb widely used in folk medicine.4 β-hydroxywithanolide E compound isolated from the P. peruviana. The study further confirmed that HWE inhibited cell proliferation and induced cell apoptosis in the human A549 cells.

β-hydroxywithanolide E化合物自秘鲁酸浆Physalis peruviana的茎及叶部位分离之纯化物。A549人类肺癌细胞分别以HWE与5-FU处理,进行抗肺癌活性先筛选出强效之抗癌药物,再进行细胞凋亡机制研究。

Reparation of PBO was begun with the polymerization process in which composite salts that contained 4,6-diaminoresorcinol and terephthalic acid were dissolved in polyphosphoric acid and phosphorus pentoxide.

BO纤维制备:以4,6-二氨基间苯二酚的盐酸盐为单体原料,与对苯二甲酸混合,经过中和反应、脱色处理、干燥后制成复合盐;再以多聚磷酸为溶剂,添加五氧化二磷和抗氧化剂,经过预聚合反应制备出PBO聚合物;聚合物通过双螺杆挤出机进一步进行聚合,然后通过喷丝板挤出,在磷酸水溶液中凝固成型,经过洗涤、干燥等过程,最终卷绕成型。

AIM: To determine the effects of posthepatic manipulative bleeding on the lung injury induced by hepatic ischemia-reperfusionin rat model.

目的: 探讨肝后控制性失血处理对肝脏缺血再灌注所致肺损伤的影响。

AIM: To determine the time course and peak production of reactive oxygen species in suprahepatic vena cava after hepatic ischemia-reperfusion and to study the effects of posthepatic manipulative bleeding on HIR injury in rat model.

目的:确定肝脏缺血再灌注时肝上下腔静脉血中氧自由基产生的时相及峰值,探讨SH-VC控制性失血处理对HIR损伤的影响。

METHODS: Rat 35 min total hepatic ischemia model was used in this study. We treated the experimental rats by posthepatic manipulative bleeding or IH-CV manipulative bleeding at 10 min after reperfusion.

用大鼠HIR损伤模型,在再灌注后的10min分别在肝上下腔静脉及肝下下腔静脉进行控制性失血处理,观察其对HIR所致肺损伤的影响。

Prakash gave time for Isrizal to settle his affairs and for Shafi'ie to complete his national military training.

普拉卡西法官允许依斯里扎先处理完手头上的事情,而慕哈末也可以先服完兵役再入狱服刑。

The results showed that, using transient GUS expression, the frequency of resistant calli and shoot differentiation as a criteria, wheat mature embryos were cultured on the preculture medium of CM4C1P for 14 days, and immersed in inoculation suspension Ⅰ for 3 hours, then the explants were co-cultured under desiccation conditions for 3 days, which was optimal conditions for Agrobacterium-mediated transformation of wheat mature embryos.

为了进一步完善根癌农杆菌介导的小麦遗传转化体系,以小麦成熟胚为转化受体,研究了根癌农杆菌介导法转化小麦的主要影响因素,结果表明,以gus基因瞬时表达率、抗性愈伤组织获得率和分化率为转化指标,小麦成熟胚在预培养基CM4C1P上预培养14d后,用侵染培养液Ⅰ侵染3h,再经干燥共培养方式处理3d,是根癌农杆菌介导成熟胚转化的合适条件。

Two pairs of primers (TS5/TS11 and S18/S19) were used for the PCR method. Positive PCR results were obtained after the 18-hour preculture (6 h in lactose broth at 37℃ followed by 12 h in Rappaport-Vassiliadis broth at 42℃) for spiked raw and irradiated meats with or without freezing treatment.

试验以经过或未经过冷冻处理之加菌的鸡绞肉及辐射鸡绞肉,采用乳糖培养液於37℃培养6 h,续以Rappaport-Vassiliadis 培养液於42℃培养12 h之预培养,再配合PCR法,均可检测出沙门氏杆菌。

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