再取得

The total RNA from brain tissue of substantia nigra of PD rats is extracted at the 1st, 2nd, 4th and 8th week and comparison is made between it and that from normal rats. The total RNA, after RNA formaldehyde degenerated sepharose electrophoresis and separation and Northern transfer, is adsorbed unto colloxylin membrane. Then ECL labeled Selenoprotein P and an unknown gene fragment are used as probes to hybridize with the RNA on the colloxylin membrane. And the hybridized result is obtained with enzyme-linked immunosorbent assay. Finally, analysis of OD is made using ONE-Dscan software.

分别提取1周、2周、1月、2月的帕金森大鼠（各8只）黑质区脑组织总RNA，并取相对应时间的正常大鼠的脑组织总RNA作为对照，经RNA甲醛变性琼脂糖凝胶电泳分离后，通过Northern转移，将其吸附在硝酸纤维素膜上，然后利用ECL标记的Selenoprotein P和一段未知基因为探针，与硝酸纤维素膜上的RNA分子进行杂交，再通过显影取得杂交的结果，应用ONE-Dscan软件进行光密度值分析。

On campus, walking among students of different ethnicities and different mentalities, I can learn humanities by full appreciating cultual differences and nicely fuse them into a mosaic.

这个国家中文程度还是不错的，重点是它中式食物容易取得。不像美国，买个菜都起码开个一小时。再不济，满街都是华人和一出门连个鬼影都看不到的滴地方一比，也不知强到哪里去。

Through the above discussment, we can conclude that in the thickness of top coal detection,elastic wave method is effective. If the focal satisfy the requirment of the frequency bnak and we take some measures on the emit and receive equipment and use advanced signal analysis method -wavelet analysis ,we can obtain ideal result .

以上工作表明，应用弹性波方法对顶煤厚度探测是切实可行的，只要震源满足频带要求，发射和接收装置采取相应的措施，再结合先进的信号处理方法—小波分析，煤厚探测是可以取得比较理想的效果的。

According to different acidity of free Flavonoids in Resina Draconis, different acidic lavonoids were extracted with the pH gradient extraction method from ethyl ether.

根据血竭中游离黄酮存在着酸性差异，采用溶剂除杂，转溶于乙醚，再用pH梯度萃取得到不同酸性的黄酮，进而对弱酸性组分分离纯化得到2个单体化合物。

In this regard, our goal is to develop a glow-discharge method to functionalize titanium surfaces by the covalent immobilization of bioactive organic molecules.

钛金属圆片先以氩气电浆去除表面污染物，来产生可重复取得的清洁表面，接著用丙烯胺电浆处理，使丙烯胺聚合在钛金属表面，再以交链剂戊二醛将白蛋白与丙烯胺的胺基(-NH2)键结。

The glottal flow derivative is estimated by using an inverse filtering technique which obtained from the vocal tract system which is established by large database of x-ray pictures and simulated by digital signal processing multiplies the frequency domain value of the original speech signals.

声门来源波型的取得是利用大量X光为基础而以数位讯号方式模拟人体口腔模型而将所需要的反函数求出再将之与原语音的频谱图做相乘而得之。

This study not only implicated a possible accession for the management of diseases characerised by pathological cell proliferation using drugs targeting PI3K/PTEN/PKB signal system, but also afforded theory evidence for the ideal results of the two agents in the prevention and treatment of atherosclerosis and restenosis after interventive myocardial reperfusion.

此项研究不仅为研制PI3K/PTEN/PKB调节药物用于以细胞过度增生为主要病理改变的疾病治疗展示了可能途径，更为该两种药物在冠心病一、二级预防或冠脉介入治疗后再狭窄防治中取得的理想疗效提供了理论支持。

The reliance of regional tourism to the information is the internal agent of tourist managemental informatization,which is the base of an electric goverment.

江西的旅游信息建设经过分化整合及经营策略的再探索，取得了一定的成效，已逐渐进入一个成熟稳健的发展时期。

All tests, including acoustic reflectometry, tympanometry, pneumatic otoscopy, and videotelescopy were performed in the two days preceding surgery. Myringotomy or tympanic aspiration was then performed to confirm the presence or absence of OME.

远距镜录影系统检查时病患可采坐姿或站姿，检查者将病患耳郭向后轻拉，再将远距镜经由外耳道放至鼓膜前，在监视器上取得清晰且放大影像。

In states with riparian rights systems, eminent domain may be used to acquire water rights by nonriparian water companies or municipalities, which can then provide a domestic water supply.

在有河岸权制度的州，不在河边的水公司或自治区可以利用土地征用权取得水权，然后再供应生活用水。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。