再发生的

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

In SMC group, relative stable of the both ends of fracture reduce unnessary repeated stimulation of injury, and at same time, axio-stress advantageous for preosteoblast and mesenchymal cell transmit to osteoblast and chondroblast. Material and design superiority of SMC form unique internal mechanical environment, and which benefit for bone healing. The study took deep investigation of SMC on fracture healing.

上述结果证实：SMC内固定可对骨折端施加主动的、持续的轴向应力刺激，形成沿长骨骨干轴向的成骨导向力值，配合环抱翼的把持作用，形成了区别于CP、IN的独特的稳定、有效的机械内环境，骨折端的相对稳定减少了不必要的损伤再刺激，同时持续轴向应力有利于成骨祖细胞、间充质细胞向成骨细胞和成软骨细胞转化、促进了成骨诱导过程的发生与发展，加快了骨折愈合的修复与重建。

A gene sequence of the anthropogenic adiponectin spherical fragment obtained by the invention absolutely accords with a known gene sequence, and compared with the full-length adiponectin, the hydrolyzed anthropogenic adiponectin spherical fragment has stronger biological activity, fast reaction after dosage, good bioavailability and better clinic application prospect, especially when the diabetes patient receives a recanalization treatment after a myocardial infarction, the anemically myocardial damage of the diabetes patients can be eased by the exogenetic dosage.

本发明公开了一种人源性脂联素球状片段用于治疗糖尿病缺血性心脏病药物的应用，本发明所得到的脂联素球状片段的基因序列与已知的基因序列完全相符，与全长的脂联素相比，其水解以后的脂联素球状片段有着更强的生物学活性，而且给药后反应迅速，生物利用度好，有着更好的临床应用前景，特别是对糖尿病患者在发生心肌梗死后接受血管再通治疗时，通过外源性给药，以减轻糖尿病患者缺血心肌损伤。

Bronchiolitis obliterans is the most disturbing complication following lung transplantation. This study is to investigate whether prolonged ischemia would promote early establishment of BO Material and Methods Left lower lobectomy was performed from miniature swine. Lung fragments, each measured 1 cm3, contained terminal bronchioles were auto- or alloimplanted into the subcutaneous area around the nipples. These lung implants were removed on 7th; 14 th; 21 th; 28th and 56th days and were fixed for histological examination. Experimental group using the same procedures but the left lower lobe was washed with 4.degree.C Lactate ringer via interlobar artery before lobar bronchus was resected.

肺移植后的阻塞性细支气管炎是肺移植后最困扰的并发症，本研究的目的希望了解缺血时间的延长是否会促进阻塞性细支气管炎提早发生实验方法施行小猪的左下肺叶切除手术，将取下的肺切成含终末支气管1 公分立方的肺小块，以自体及异体异位肺移植模式，将植入小猪皮下的肺小块於植入后第7、14、21、28 及56 天取下，在病理下检示其病理变化，并利用原位灌留的方式将左下肺叶以4℃林格氏液灌洗后再摘除之，观察有无灌洗对於阻塞性细支气管炎生成的影响。

Method］from january 2003 to may 2006,32 patients were corrected with qin si-he's orthotics devices on the ilizarov principle of tension-stress,which involved 15 males and 17 females,the age ranged from 10 to 25 years.among these patients,2 were caused by peroneal nerve injury,l by tumor in the vertebral canal,5 by meningocele,11 were caused by poliomyelitis,13 by congenital talipes equino-varus.in accordance with deformities,external fixator and limitied operative methods were dertermined.the limited release of soft tissue were performed in 7 patients,limited osteotomy in 25 patients.the dynamic muscle balance operation were performed in 9 patients with imbalance of muscle strength.according to the ilizarov technique,the fixative rods were installed.the telescopic rods on the apparatus were rotated one week after the operation,the divices had corrective function in three-dimensional directions.the deformity of talipes equinovarus,internal rotation and drooping of the forefoot were gradually corrected,and the patients could bear weight and walked on the deformed foot.the mean duration of traction were 42 days,then removed the external fixator maintained with plaster for a site time.

方法］2003年1月～2006年5月，根据ilizarov张力应力法则，应用秦泗河改良的外固定矫形器，遵循ilizarov穿针固定的基本原则，共手术治疗马蹄内翻足32例，男15例，女17例；年龄10～25岁，平均17岁。病因：腓总神经损伤2例，腰椎管内肿瘤1例，硬脊膜膨出5例，小儿麻痹后遗症11例，先天性马蹄内翻足13例。术前用足掌的前外缘负重行走者11例，用足的外缘或足背外侧负重者21例。根据马蹄内翻足畸形程度、性质和患者年龄，确定实施有限矫形手术的方法和外固定矫形器治疗。本组7例同期实施有限的软组织松解术，25例同期实施了有限的截骨术和跗骨间关节融合术，9例合并踝关节内外翻肌力明显失衡者，同期行足部肌腱转移的肌力平衡术。然后安装外固定矫形器。术后按作者制定的管理程序，7 d开始旋转相应的螺纹牵拉杆，对器械进行三维空间的缓慢调整，先矫正前足内收和后足内翻，后矫正足下垂畸形，直至达到矫形要求的标准。在矫形的过程中定期进行x线检测，以防止发生踝关节前后移位，治疗期间允许患足负重行走。术后平均牵伸42 d，拆外固定器后患足再上石膏固定适当时间。

Although the EPRI give a set of the approximate engineering solution method, actually the crack of parts in engineering project mostly presents inner 3D effect. If using 2D elastic-plastic or 3D elastic analysis, the result can't be precise. Because these methods have neglected the reassignation of the stress and strain which happened at nearly the top of cracks, carrying on 3D elastic-plastic analysis and J-integral calculation become the foundational work to study the expander and safety assessment of the pressure vessel cracks.

虽然EPRI给出了一套近似的工程解法，但实际上工程中构件的裂纹大多呈现内在的三维效应，若使用二维弹塑性或三维弹性分析必然不够精确，因为对裂纹的&等效&二维表达忽视了发生在靠近裂纹端部的应力和应变的再分配，所以对压力容器裂纹进行三维弹塑性分析及其J积分是研究压力容器裂纹扩展和安全评定的基础工作之一。

China's railway is developing along with the society's development, from coal as the driven power to electricity, from ballasted track to Non-ballasted track, from low-speed to high-speed , China's railway has undergone earth-shaking changes, and now the construction of high-speed railway leads to the takeoff of China's economic and social , Although high-speed railway in China start late , but have a high starting point, and developed very quickly, through introducing foreign core technology, digestion, absorption and re-innovation ,we owned the ability to build the high-speed railway, and ushered into a new era of high-speed railway construction.

我国的铁路随着社会的发展而发展，从以煤为动力到以电力直接带动，从有砟到无砟，从低速到高速，我国的铁路发生了翻天覆地的变化，而如今高速铁路的建设正在引领我国经济社会的腾飞，我国高速铁路虽起步晚，但起点高、发展快，通过引进国外核心技术、消化吸收再创新，初步具备了建设高速铁路的能力，迎来了我国高速铁路建设新时代。

Yang Zhichao's "Macau" attempts to show the whole process of how the blood of the artist loses the original environment and the inherent functions of blood after experiencing well-technology-based "drift" and thus becomes dissimilated in shape and purpose : the blood in the body of the artist is quantitatively exsanguinated for three times within 45 minutes and then poured into a dice-shaped mould, and the dice is taken out after the blood curdles.

杨志超的《澳门》试图展示艺术家本人的血液历经精心技术化的&漂流&之后，如何失去了血液流通的原有环境和固有功能，从而在形状和用途上发生异化的全过程：艺术家体内的血液，被分成三次定量采撷，历时45分钟，而后灌入骰状模具，待血液凝固后，再取出翻好的骰子。

It is not clear, however, to what extent these mechanisms might overlap because there is some evidence that the local stimulus of more frequent milking can, in turn, cause more nutrients to be delivered to the udder at the expense of other tissues (DePeters et al., 1985; Kazmer et al., 1986), although there is no evidence of major repartitioning comparable to that seen with growth hormone injections.

Bauman 和弗农，1993它不清楚，然而，到什么范围因为有比较时常发生挤奶的当地刺激能，依次的一些证据，所以这些机制可能重叠，引起要递送到乳房的较多的营养物牺牲其他的薄纱织品(DePeters 及其研究同仁，1985； Kazmer 及其研究同仁，1986)，虽然没有对于主修科目的证据再分配可比较的对那与生长荷尔蒙注入一起见到。

On the basis of experiment analyses we would got that, When arc met with chute, it was blocked and had a stagnation, this made hot gas flow incrase near the chute; After arc went into the chute, mental vapor from arc root would lower the dielectric strength near the chute, meanwhile, High value of arc voltage and the corase face of the chute made electric strength increase. These factors might give rise to local restriking near the chute, and made arc voltage drop suddenly.

总结实验结果并加以分析，本文提出了这样的观点：电弧进入栅片时，在入口处受阻后的停滞，会使该区域的热气流增加；进入栅片后，弧根蒸发的金属蒸汽、电弧热气流会降低栅片入口区域的介质强度；电弧被栅片分割后，电压急剧上升，再加上栅片表面的不均匀会使局部区域的电场强度显著增加，这些因素的综合作用会导致电弧在栅片入口附近发生局部重击穿，引起电弧电压电压跌落。

By the time of its fall, most of the prisoners were writers who had written against the corruptions of the government.

到它被攻陷的时候，里面多数的犯人是写了反对政府贪污文章的作家。

The most obvious variation to ovum morphological character was that the color was changed from light green to sepiaceous in embryonic development, and all the ovums were almost hatched after 96h.

在胚胎发育过程中卵的形态特征最明显的变化是颜色从淡绿到深褐色，卵在发育96h后卵基本全部孵化。