再发生

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

Autoreduction of Pt〓 ionsin β zeolite is found directly by means of CO-IR and TPR methods.

一。首次系统地研究了β沸石上Pt胺化合物经分解、还原及再氧化等处理过程中发生的变化，并提出关于Pt粒子大小及其状态变化的模型。

If the parties had waited for two more days and not stalled the movement by accepting the April 24- 25,(11 Baisakh) announcement, the October revolution might have happened right then.

如果各政党再多等两天，而不是接受4月24日，25日的宣言，结束了人民运动，那么十月革命当时就发生了。

If the parties had waited for two more days and not stalled the movement by accepting the April 24- 25,(11 Baisakh) announcement, the October revolution might have happened right then.

如果各政党再多等两天，而不是接受4月24日，25日的宣言，结束了人爱猫扑。爱生活动，那么十月革命当时就发生了。

Buffett is able to do this because of his credit rating, and if there are no claims, he puts the 5% in his pocket,' said Andrew Barile, a reinsurance consultant in Rancho Sante Fe, Calif.

加州再保险咨询师巴瑞尔说，巴菲特能这么干是凭他的信用评级，如果没有发生赔付，他就赚了5%。

Twinkle twinkle can't you see need you need you but let you go somebody said to me that life is a mysiery and that you never know what's out there awaiting for you everything is meant to happen, to write a story of life even when you are so alone even when you are so afraid with this loneliness you'll be alright 'cuz it will lead your way come on twinkle why don't you clap your hands just open your door for the belter tomorrow and play your own life like you're a movie star don't you know that we're all dreamers twinkle twinkle can't you see need you need but let you go the future's in your hands and heres the map showing the way to find yourself again, a part of you we never knew tell yourself it'll be alright to give up and swallow your pride believe in yourself don't be afraid all your worries will go away because you will fight to win your life with strength to carry on come on twinkle why don't you clap your hands just open your door for the better tomorrow and play your own life like you're a movie star don't you know that we're all dreamers suddenly the sadness took my soul away like a rain on perfect day but let me go and live my life come on twinkle why don't you clap your hands play your own life like you're a movie star come on twinkle why don't you clap your hands just open your door for the better tomorrow and play your own life like you're a movie star don't you know that we're all dreamers and we walk out the door.

闪耀闪耀你不能看见需要你需要你但是让你去有人向我说生活是一mysiery 和你从不知道所的什么无法使用那里为了你等待每件事被意味着发生，写生活的一个故事甚至当你是如此孤独的甚至当你这孤独的如此害怕你将是alright'cuz它将领导你的方式在上来闪耀为什么你不拍击你的手公正敞开你的地带的门明天和玩你像你一样的自己生活是一个电影明星你不要知道我们都是梦想者闪耀闪耀你不能看见需要你需要但是让你去未来你的手同时，继承人显示方式的地图再一次为了找到你自己，一部分你我们从不知道告诉你自己它将是alright 为了放弃并且吞咽你的骄傲相信你自己不害怕所有你的担心将离开因为你将战斗赢得你的生活具有力量继续在上来闪耀为什么你不拍击你的手公正敞开你的门更好明天和玩你像你一样的自己生活是一个电影明星你不要知道我们都是梦想者悲哀突然地拿我的灵魂像一场雨一样完美天但是，让我去并且过着我的生活在上来闪耀为什么你不拍击你的手玩你像你一样的自己生活是一个电影明星在上来闪耀为什么你不拍击你的手公正敞开你的门更好明天和玩你像你一样的自己生活是一个电影明星你不要知道我们都是梦想者同时，我们出去door。

The staining of F-actin in the ischemia reperfusion group was decreased significantly （P.05） compared with the normal control group. There was a marked loss of canalicular microvilli and distension of canalicular lumen in the ischemia reperfusion group. The staining of F-actin in the LA group was significantly increased than those in the I/R group （P.05）. Conclusions: Ischemia reperfusion induces an disruption of bile canalicular F-actin microfilaments and a loss of microvilli. Alpha-lipoic acid can protect bile canalicular F-actin microfilaments against IRI.

肝脏I/R可造成胆小管F-actin微丝破坏、微绒毛丧失，导致胆小管收缩减弱，胆汁排泄功能受损，这可能是大鼠肝脏I/ R后缺血型胆道病变发生的主要机制；α-硫辛酸通过清除氧自由基，降低脂质过氧化反应，调节细胞内氧化剂-抗氧化剂的含量，保护胆小管F-actin微丝结构免遭缺血再灌注损伤破坏，进而预防和治疗缺血型胆道病变。

I kept coming up with useful facts about what would be happening to a kangaroo foetus at the particular stage that our baby was also at. I'm not entirely sure she found that helpful. Actually, I think she got a bit annoyed. She'd say,"Would you stop with the stories about cannibalistic sharks. It isn't helping."

我不听地告诉她一些有关在我们的宝宝也会处于的一特定阶段袋鼠胎儿会发生的一些有用事实，我不是完全确定这些是否能对她有所帮助，实际上，她对此有些恼火，她会说，'你能不能不要再对我讲有关鲨鱼吃自己同胞的故事'。

By sulphonation of the PS-DES phase with chlorosulphonic acid, strong cation-exchange stationary phase of sulphonic type was obtained.

所产生的PS-DES再和氯磺酸发生磺化反应，即得到磺酸型的强阳离子交换型固定相。

In SMC group, relative stable of the both ends of fracture reduce unnessary repeated stimulation of injury, and at same time, axio-stress advantageous for preosteoblast and mesenchymal cell transmit to osteoblast and chondroblast. Material and design superiority of SMC form unique internal mechanical environment, and which benefit for bone healing. The study took deep investigation of SMC on fracture healing.

上述结果证实：SMC内固定可对骨折端施加主动的、持续的轴向应力刺激，形成沿长骨骨干轴向的成骨导向力值，配合环抱翼的把持作用，形成了区别于CP、IN的独特的稳定、有效的机械内环境，骨折端的相对稳定减少了不必要的损伤再刺激，同时持续轴向应力有利于成骨祖细胞、间充质细胞向成骨细胞和成软骨细胞转化、促进了成骨诱导过程的发生与发展，加快了骨折愈合的修复与重建。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。