再制备

By partially thawing the prefrozen samples and refreezing them in liquid nitrogen before the final liyophilization, we prepared biomimetic multilayered scaffolds.

采用-20℃冷冻后，部分熔融再液氮重冻的预冻方式，冷冻干燥制备了厚度约2mm的仿生多层软骨支架。

Using the byproduct,namely,the corn skin and grit produced from corn starch and protein extraction as the material of the saccharifying koji fermentation,and then hydrolysing the corn skin and grit with this kind of saccharifying koji,the saccharified solution was obtained.

利用玉米提取淀粉和蛋白质后的玉米皮渣副产品做为糖化曲发酵原料，再利用糖化曲水解玉米皮渣制取糖化液，补充氮源后制备液体酒母培养基，进而利用该培养基代替麦芽汁培养基生产液体酒母。

After starch and corn protein was extracted, corn skin and grit was used as materials for saccharifying koji and vinegar fermentation.

研究了以玉米提取淀粉和蛋白后获取的玉米皮渣为糖化曲制备原料和食醋酿造原料，采用边糖化边酒化、再醋化工艺，固态法酿造食醋。

Then the aluminium sulfates were prepared by sulfation and salt formation, and their IR spectra were analyzed.

在此基础上再经硫酸酯化和成盐修饰制备了各自的硫酸酯碱式铝盐。

First, with the gelation property of alginate molecule in Ca2+ solution, collagen/alginate threads were prepared through thermally controlled syringing collagen/alginate (w/w=1:3) solution into 2% CaCl2 solution.

第一阶段利用褐藻酸高分子在钙离子水溶液的环境下迅速成胶的特性，再运用湿式纺织技术，将胶原蛋白与褐藻酸混合溶液(胶原蛋白重：褐藻酸重＝1：3)经由恒温注射针筒系统以2ml/min的流速挤压至氯化钙水溶液(2%)的成型槽中，并控制罗拉转速，制备胶原蛋白/褐藻酸细丝。

The products were identified by IR,NMR,MS and elemental analysis. In our method,K.OH was used in place of NaOH to synthesize benzyl ester of 3,5-dimethyl-4-hydroxybenzoic acid,2',6'-dimethyl-4'-(n-succinimidyloxycarbonyl) phenyl-acridinium-9-carboxylate was purified on a silica gel column with chloroform/ethylacetate(4:l,v/v) as eluent and further purified by triturating with hexane/acetone(2:l,v/v).The luminescence produced by DMAE-NHS is a flash light with maximumemission at 0.4s and decay half-time of 0.9s. The luminescence intensity is 6.11x10 cps/mol,which is affected by the composition of trigger and surfactant.

DMAE·NHS的合成是本论文的关键和难点，我们对文献方法进行改进：文献方法用氢氧化钠与3，5-二甲基-4-羟基苯甲酸反应制得3，5-甲基-4-羟基苯甲酸钠，再用3，5-二甲基-4-羟基苯甲酸钠与苄氯作用制备3，5-二甲基-4-羟基苯甲酸苄酯，我们用氢氧化钾代替氢氧化钠，使合成取得成功；在合成2'，6'-二甲基-4'-苯基-吖啶-9-甲酸酯时，文献方法对粗产品进行两次硅胶柱层析纯化，第一次柱层析用氯仿/乙酸乙酯(4：1，Ⅴ：Ⅴ)作溶剂和淋洗剂，第二次柱层析用己烷/丙酮(2：1，Ⅴ：Ⅴ)作溶剂和淋洗剂，按照文献方法得到的不是所需要的化合物，我们只进行第一次柱层析纯化，然后用己烷/丙酮(2：1，Ⅴ：Ⅴ)进行研磨，过滤，洗涤，除去溶于己烷/丙酮(2：1，Ⅴ：Ⅴ)的部分，得到所需要的产品。

We have synthesized 4,4'-dinitro-3 4''-dimethyl triphenylamine,(1), in 84% yield in an one-step condensation process between 3,4-dimethyl aniline and 4-fluoro-nitrobenzene. After the auto-oxidation of (1) and the subsequent hydrogenation of the nitro-groups, an AB2 type triphenylamine polyimide intermediate bearing two amine groups and one acid-anhydride group each on individual phenyl ring,{AB2 PI Intermediate}, was prepared successfully. The yields of the autoxidation and the hydrogenation steps are 81% and 90% respectively.

中文摘要本研究以3，4-二甲苯胺及4-氟硝基苯为原料，经一步骤缩合反应得到产率为84 %之4，4'-二硝基-3&，4&-二甲基三苯胺(1)，由(1)经自氧化法后再以氢化法还原硝基，成功地制备出带有二胺基与一羧基的三官能基之AB2型三苯胺中间体(AB2型PI中间体)，自氧化反应与氢化法的产率各为81%及90%。

The HIBD model was established by temporarily occluding the right common carotid artery for 3 hours with a thread through an elastic tube, and then they were exposed to the gas mixture of 80 ml/L oxygen and 920 ml/L nitrogen for 1 h.

经弹性管穿线阻断右颈总动脉3h，低氧(8％O_2和92％N_2混合气)1h，制备HIBD模型。3h后剪开扎线予再灌注，彩色多普勒监测右侧颈总动脉血流。

From the first experimental week, nine geese per groupwere weighed on an empty stomach ,and collected samples for further assess on 7th in every week;From the third experimental week, excepting for weight, three geese per group were randomly selectedand collected blood in jugular vein, then separated serum and assessed blood, endocrine andimmunological index; after collecting, geese were killed and autopsied, Spleen, bursa of Fabricius andThymus were collected for relative weight of immune organs of goose, calculated immune organs index.

从试验第一周开始，每周的第7日每组随机选取9只鹅空腹称重；统计耗料量；从试验第三周开始除称重外，每组再随机选取3只，颈静脉采血5ml，制备血清并测定血液、内分泌和免疫指标；采血后宰杀取脾脏、腔上囊和胸腺，称重，计算免疫器官指数。

Then the experiments on the separation of lithium and magnesium from bitterns contenting lithium were carried out, during the experiment NaOH was chosen as addition to further remove magnesium from the extraction liquid and Na_2CO_3 was chosen as deposition agent to prepare Li_2CO_3 product.

作者选取磷酸三丁酯为萃取剂，200#溶剂汽油为稀释剂，氯化铁(FeCl_3)为共萃取剂，对盐湖含锂卤水进行萃取分离镁锂的试验，然后采用氢氧化钠对萃取液进行深度除镁，再用碳酸钠作为沉淀剂制备碳酸锂产品。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐