再制备

Expanded MSCs were induced to differentiate into nerve cells withthree different treatment protocol in the prensent study . On the following day, the medium was replaced with preinduction medium consisting of DMEM, 20% FCS, and 10 ng/ml basic fibroblast growth factor. After 24 h, the preinduction medium was removed, the cells were washed twice with PBS, and neuronal induction medium containing DMEM supplemented with 2% DMSO and200 m bu-tylated hydroxyanisole was added. In later experiments, we used DMEM with 5 mM p - mercaptoethanol or sulfo - glycerine as an alternative neuronal induction medium for the same incubation.

将待诱导分化的MSCs按0.4x10~6／ml接种于事先放置有消毒盖玻片的六孔板内制备细胞爬片，分别采用三种不同的方法诱导第2代至10代的MSCs向神经细胞分化，即方法1先用含bFGF的培养基进行预诱导，之后再用含叔丁基对羟基茴香醚，和二甲亚砜的无血清培养基进行诱导；方法2和方法3用B-巯基乙醇和硫代甘油进行预诱导，然后不同浓度的上述物质进行正式诱导。

According to the result showed at 280nm and at 490nm,in the comparison of whether protein absorption top and sugar quantity top overlapped,glycoproteins would be detected preparatorily,and as a result,tubes in two distinct areas had glycoproteins by this method. Proteins were precipitated with trichloroaceticacid and with cold acetone,and glycoprotein was determined from SDS-gel.

再从各收集管的收集液中，用三氯乙酸沉淀蛋白法、冷丙酮沉淀蛋白法相结合浓缩、制备蛋白样品，进行SDS—PAGE，对SDS胶进行PAS糖链染色鉴定糖蛋白，并从茶树叶分级蛋白中准确地鉴定出两个区域的收集管中含有多种糖蛋白。

A series of polyimide/talc composite films with different contents of talc were prepared via thermal imidization of in-situ polymerized poly/talc solutions based on pyromellitic anhydride, 4,4'-oxydianiline and talc.

用原位聚合法合成一系列不同滑石粉含量的聚酰胺酸／滑石粉溶液，再将其热酰亚胺化制备出聚酰亚胺／滑石粉复合薄膜。

The synthesis of water reducing agents from phenanthrene waste by sulfonation,condensation,neutralization and recondensation has been studied by the orthogonal test.

利用正交实验通过磺化、缩合、中和、再缩合反应对菲渣制取水泥减水剂进行了研究，优化了主要工艺条件，制备出的减水剂减水率可达17.5%。

He TiC could reprecipitate again with excess milling and Cu crystal decreasing. TiC-dispersion-strengthened Cu-based composite powders have been prepared from TiC-Cu and Cu-Ti-C mixed powders by MA. The composite powders were prepared into composite materials by pressing, sintering and heat-extrusion.

IC混合粉末经机械球磨后，再经压制、烧结和热挤压制备成 n 弥散强化铜基复合材料。

In this thesis, using a low molecular weight polymer TPA-PPV and a high molecular weight polymer PVK as the model compounds, a series of highly monodisperse nanoparticles with controlled size and shape had been prepared by reprecipitation method and a new method respectively.

本论文以小分子量聚合物TPA-PPV和大分子量聚合物PVK为目标化合物，分别用再沉淀方法和一种新的方法成功制备了一系列不同尺寸的、高度单分散的聚合物纳米超微粒，并研究了PVK纳米球二维阵列的自组装过程。

In part one, we prepared three ramification of pyrene nanoparticles by reprecipitation method under ultrasonic radiationand characterized with TEM.The determination of organic nanoparticles to DNA, NO2 -,and nucleic acids werestudied by using fluorescence. And some ultrasensitive determination of DNA, NO_2~-, and nucleic acids methods were proposed. The possible mechanism has been studied.

第一部分在超声辐射下用再沉淀的方法制备了几种芘的衍生物的纳米粒子荧光探针，并通过透射电镜进行了表征，并通过荧光法研究了它们分别与蛋白质、核酸以及NO_2~–的作用，建立了超灵敏的检测上述物质的方法，探讨了作用机理，并提出了合理的解释。

The results show that the resulting product can be separated by the mixture of petroleum ether, aether and acetic acid (10:10:1, V/V). Among them, the oleic acid component can be eluted by the mixed solvent of ethanol and carbon tetrachloride (35:15, V/V), and then productivity of sodium oleoyl sarcosine can be obtained.

结果表明：用石油醚：无水乙醚：冰乙酸＝10：10：1分离产物中的组分，再用无水乙醇：四氯化碳＝35：15混合溶剂洗脱制备油酸组分，进而计算油酰肌氨酸钠的产率是可行的。

In the invention, normal reversible absorbing transfer material for solar energy which are mixture of cobalt chloride and sextic methyl tetramine, phthalandione lipid compound and trilateral methane lactone compound, said material are produced to micro cachet that colour changes as stemp, changing reversely in normal temp, then said micro cachet are compounded with common white silicate cement to produce the invention cement base material.

本发明采用对太阳能呈常温可逆吸收转换材料：氯化钴与六次甲基四胺混合物、邻苯二甲酸酯类化合物和三芳甲烷内酯类化合物，将上述材料制成微胶囊，再将所制出的常温可逆温致变色微胶囊与普通白色硅酸盐水泥复合，即可制备出常温可逆温致变色水泥基材料。

Methods PP/SiO2 nanocomposites are produced by melting-blend process and then the best dosage of silicane KH-560 is discussed by analyzing its mechanical behaviors and SEM photo graghs.

方法首先用熔融共混法制备 PP/SiO2 纳米粒子复合材料，再通过分析复合材料的力学性能和冲击试样断面的 SEM 照片来探索硅烷 KH-560 的最佳用量。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐