再分析

Method］ Alcohol solvent extraction method was used to extract the total extract of Thermopsis lanceolata, and it was treated by acid-soluble and alkalization. Then chloroform was used to extract the total alkaloids. Finally, GC-MS was used to analyze and identify the chemical composition.

方法］采用醇类溶剂提取法，提出披针叶黄华总浸膏，经酸溶、碱化处理，再用氯仿萃取出总生物碱，利用GC-MS法对其化学成分进行分析鉴定。

In 1973 the Institute of Chemical Metallurgy was engaged to carry out research on fluidizcd roasting of titaniferous iron ore containing vanadium, with the special objective of utilizing the local resources of natural gas and hydraulic electric power, by reduction of the ore with a hydrogen-rich gas followed by melting of the reduced cinder in an electric furnace in order to separate the iron from a slag rich in vanadium and titanium.

化工冶金研究所于1973年接受了&流态化焙烧还原钒钛铁矿&的任务，要求结合当地资源，用天然气还原铁精矿，用水力发电熔化焙砂，再从熔渣中提取钒和钛。我们对流态化还原的若干方面开展了试验研究，包括铁精矿还原反应数据的获得和有关分析，以及可供应用的两种流态化反应器的工程研究。第一种是气控式多层流态化床，第二种是快速流态化反应器。此外，对于流态化还原中的失流以及熔砂的渣～铁分离和从熔渣中提取钒钛，也进行了试验研究。

The researcher then surveyed another 767 subjects to examine the relationships of Taiwanese love styles with gender, sex-role, personal modernity and traditionality, relationship satisfaction, and western love styles.

其次再调查七百六十七名样本，针对八种爱情风格及其性别、性别角色、个人传统性与现代性、关系品质以及西方的六种爱情风格做t-考验、变异数分析或相关统计。

The founded models were applied to the calculation of the molten salt actinide recycler and transmuter system, in which the neutronics, thermal hydraulics and safety characteristics were obtained, and the influences of the inlet temperature, inlet velocity and the resistant time of the fuel salt in the external loop were analyzed.

将建立的数学模型应用于锕系元素再循环嬗变熔盐堆的计算，对其堆芯物理特性、热工水力特性和安全特性进行了系统分析，考察了入口温度、速度及燃料盐在堆芯外运行时间的影响。

The molding processes of complex coextrusion interface such as column, rectangle, hatch trapezia and inlay shape, were analyzed. It is showed that the interfacial appearances obtained by the simulation results are quite matched with the results of experiment.

同时通过分析指出确定工艺参数时应首先根据材料的特性(主要是两种聚合物的剪切粘度)和型材截面形状确定合理地入口流量比，然后再根据设备性能、熔体允许的压力降等条件确定两种熔体的入口流量。

The effects of technologic parameters, including concen- tration and temperature of ADP solution and galvanizing time on rising time of anodic oxide films are discussed. 2. After dipped in amino trimethylene phosphonic acid solution, anodic oxide films are annealed in pipe stove.

将化成箔在氨基三甲叉膦酸溶液中浸渍，取出烘干后再进行热处理，研究了 ATMP 溶液浓度、浸渍温度和热处理温度对氧化膜升压时间、比容、耐电压的影响，找到其最佳耐水合处理工艺；采用FT-IR对水合作用后的 ATMP 处理氧化膜和未经耐水合处理氧化膜进行了分析。

New method that Biological Tissue Section tryptic digestions in situ coupled with imaging mass spectrometry was established.

通过正交设计实验，优化实验条件，我们建立了在福尔马林固定组织切片上加胰蛋白酶液(0.1ug/ul)，60℃超声10分钟进行修复提取，再直接质谱扫描分析的新方法。

Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, ChinaAbstract: Desorbing bacterium from suspended particle surfaces by adding some different chemicals, such as Zwittergent3-12,EGTA and tryptone etc., and ultrasonic surging, comparing the difference of HPC value between before and after desorption, and then analyzing the size and number of suspended particles, it can be concluded that the effect of suspended particles in drinking water on HPC determination is significant.

本试验采用添加有助于解吸的化学试剂结合不同超声时间、超声温度下的超声波振荡对吸附在颗粒物上的细菌进行解吸，对比解吸前后HPC测定结果的差异，再对颗粒物数量、粒径进行分析，阐述颗粒物对HPC测定结果的影响。

According to the development characteristics of regional caprocks,great efforts have been made to analyse the effect of it on oil and gas accumulation and preservation in different aspects. We consider regional caprocks to be the good barriers to prevent oil and gas in free phase from escaping. If uncompacted and possessing abnormal pore fluid pressure,regional caprocks are the barriers to prevent oil and gas in water dissolving phase from escaping.

根据区域性盖层的发育特征，从不同侧面对其在油气聚集与保存中所起的作用进行了深入分析，认为区域性盖层通常是阻止游离相油气逸散的良好区域物性屏障；如果其欠压实，具异常孔隙流体压力，它还可以成为阻止水溶相油气逸散的区域压力屏障；如果其再具生烃能力，还可以成为阻止扩散相天然气逸散的区域浓度屏障。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。