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METHODS: Thirty six SD female rats were randomly separated into 3 groups (n=12 in each one) according to the infarction duration (1, 3, 28 d), which were randomly and evenly again separated into 2 subgroups: operated group and sham operated group. The levels of mRNA expression of ACE2 in the noninfarct and infract area of left ventricle 1, 3, 28 d after AMI were detected by RT PCR and analyzed semi quantitatively by image analysis system.

将36只SD雌性大鼠按时间段随机分为3组(每组12只),每组再随机分为假手术组、手术组2个亚组(每组6只),应用RT PCR方法检测大鼠AMI后1,3,28 d左心室梗死区及非梗死区心肌ACE2 mRNA的表达,并以图像分析系统对其进行半定量分析。

Total RNA was extracted from venom cells from Gloydius intermedius, FLE gene was amplified by RT-PCR. Then it was connected to pMD18-T vector by TA cloning. At last, sequencing and analysis the genesequence.

从中介蝮蛇毒腺细胞中提取总RNA,通过RT-PCR法扩增纤溶酶基因,与pMD18-T载体连接进行TA克隆,再进行序列的测定并且对该序列进行分析。

Methods:Total RNA was extracted from venom cells from Gloydius intermedius,FLE gene was amplified by RT-PCR.Then it was connected to pMD18-T vector by TA cloning.At last,sequencing and analysis the gene sequence.

材料及方法:从中介蝮蛇毒腺细胞中提取总RNA,通过RT-PCR法扩增纤溶酶基因,与pMD18-T载体连接进行TA克隆,再进行序列的测定并且对该序列进行分析。

To analyzed the phenomenon that enterprise using error correction to adjust profit caused for inveracious accounting information, financial false, this phenomenon caused by some motivation such as avoid consecutive losses, maintain the qualification of refinancing and so on.

前企业进行会计差错更正所产生的观念偏差,利润操纵等问题进行了深刻剖析,分析了企业出于避免连续亏损,保持再融资资格等动机利用会计差错更正调整利润造成会计信息不实,财务虚假的现象。

At the same time, this article deep analyzed that on the current enterprise has bias of concept in the process of accounting error corrections, manipulation of profits and other issues. To analyzed the phenomenon that enterprise using error correction to adjust profit caused for inveracious accounting information, financial false, this phenomenon caused by some motivation such as avoid consecutive losses, maintain the qualification of refinancing and so on.

同时,本文对目前企业进行会计差错更正所产生的观念偏差,利润操纵等问题进行了深刻剖析,分析了企业出于避免连续亏损,保持再融资资格等动机利用会计差错更正调整利润造成会计信息不实,财务虚假的现象。

Chromatographic fingerprint index F and chromatographic fingerprint relative index Fr were used as the objective functions for the evaluation,and the extract of Saussurea involucrate by water was used as the sample.

以色谱指纹图谱指数F和色谱指纹图谱相对指数Fr作为评价毛细管电泳分析系统的目标函数,以雪莲药材水提取液为样品,考察一定浓度的硼砂、硼酸、磷酸氢二钠和磷酸二氢钠溶液按三角形优化法和四面体优化法构成背景电解质时对样品的分离情况,通过添加有机改性剂和调节pH进行再优化。

Directly reading the 210-layer of 0.65-mm-thick consecutive CT Dicom format images of human body thoracolumbar making use of 0.625-mm isotropous resolution thin layer scanning technique into the Mimics. Defining bone organization threshold, withdrawing each outline, partitioning each edge of layer picture, edited selectively and repaired by hole processing. After a redundancy data was cleaned, the thoracolumbar 3-D finite element geometry model was acquired. Then area mesh could be kept as the document Ansys named .lis as the suffix and be introduced into Ansys software directly to mesh body, and then transferred the body mesh to mimics to assignment son the basis of CT values, once again import Ansys to establish 3-D finite element model.

将以各向同性分辨率0.625 mm薄层扫描所得的层厚0.65 mm人体胸腰段连续断层210层Dicom格式CT图像,直接读入Mimics后界定骨组织阈值、提取各层面轮廓线、图像边缘分割、选择性编辑及补洞处理,去除冗余数据,三维化处理后获得胸腰段三维几何面网格模型,将其保存为后缀名。lis的Ansys文件,直接导入Ansys有限元分析软件进行体网格划分,再将体网格转入Mimics根据CT值给予赋值,再次导入Ansys生成有限元模型。

The Hilbert amplitude envelope of the stator current includes the low frequency of fault characteristic, thus it can reduce the signal length by resample the Hilbert amplitude envelope of the stator current by downsampling, which can avoid spectrum aliasing. Then it can reduce the computation time of the MUSIC Method for the spectrum analysis to the resampled signals for the given bandwidth. Meanwhile combining continuous subdivision Fourier transform and rotor slot harmonics based slip estimation it can in advance estimate the concrete values of the fault characteristic frequency, and then inquiry purposely the fault feature component information in the stator current spectrum in order to accomplish the broken rotor bar fault with high sensitivity and h reliability, which eliminates adverse effect of issuable false peaks to the fault detection and formed a novel scheme to detect broken rotor bar fault.

定子电流Hilbert幅值包络信号包含了低频的故障特征频率调制信号,故通过对定子电流Hilbert幅值包络信号进行降采样率重采样、减少分析信号长度,可避免低频段频谱混叠,再对减少的重采样数据进行具有超分辨率的MUSIC低频段估计,可大大降低MUSIC算法频谱估计时间;运用连续细化傅里叶变换和转子齿槽谐波转差率估计技术,可预知故障特征频率精确值,从而可有目的地查询故障分量,消除MUSIC虚假波峰对故障检测混淆的影响,大大提高了故障检测的灵敏度和可靠性,以此形成异步电动机转子断条故障检测新方法。

In this study, an index evaluation system of itaconic acid was established according to the current situation of IA and the correlative laws in China. For the first time, a complete research on the IA production from this point of view was carried out, finding out the problems in the process of IA production, and then the project was advanced. All the research were begun with the data from laboratory and escalated to the industry scale. During the past three years, some of the work were applied successfully to industry.

根据现有衣康酸生产的现状和我国清洁生产相关的法律法规,建立了衣康酸清洁生产的指标评价体系,并以此为依据对现有衣康酸生产的工艺首次从清洁生产的角度进行了系统的研究,找出现有工艺中存在的问题,分析问题产生的原因,并提出衣康酸清洁生产工艺改进的方案,本研究沿着先从实验室研究规模入手得到相关的基础数据,再以此为依据逐步扩大实验规模直至在大规模生产中取得应用,通过三年的研究与实践,其中的多项研究成果已经在生产中得到应用,并取得了良好的效果。

Then simulation and analysis are performed iteratively until optimum design and operation conditions are retrieved.

用校正过的数据再进行模拟、分析,直至最终实现设计和操作的最优化。

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Chapter Three: Type classification of DE structure in Sino-Tibetan languages.

第三章汉藏语&的&字结构的类型划分。