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Methods: CD34(superscript +) cells from fetal liver were isolated with a magnetic cell sorting kit and were cultured. Cells pretreated with or without protein kinase C inhibitor chelerythrine chloride (3 μmol/L) were induced by 5 ×10^(-7)mol/L tretinoin for 24 h, and then incubated in serum-free medium. Expressions of genes in treated cells were assayed by Western blotting and RT-PCR.

采用免疫磁珠法分离人胎肝CD34细胞,培养4d后,蛋白激酶C特异抑制剂chelerythrine chloride(3μmol/L)处理24h,再加入维A酸处理24h5×10^(-7mol/L,无血清培养基培养5d,Western印迹和半定量RT-PCR法分析维A酸处理前后神经特异基因表达。

Part two: Using chloroformic ester reacted with potassuim thiocyanate to get thiocyanic esters. Then reacted with biological substituted pyrimidinamine to synthesize forty two thiourea compounds, thirty eight of them are new compounds. Their structures have been charactered by IR, 1H NMR and elemental analysis.

第二部分:先以氯甲酸酯和硫氰酸钾为原料合成异硫氰酸酯,再与有生物活性的取代氨基嘧啶反应,合成了一系列新的取代嘧啶基硫脲类化合物,合成的42个化合物中有38个为首次合成的新化合物,结构经元素分析、IR及~1H NMR得到确证。

The effect on circumgyration of carbon grain during re-entry of nose-tip is analyzed according to thermaltransfer theory,thermo-aerodynamics and N-S equation.

根据气动热力学、传热传质学、N-S 方程等分析了端头帽再入时碳颗粒的旋转效应,研究结果表明碳颗粒的旋转有利于防热。

The effect on circumgyration of carbon grain and the ablation of nose-tip during re-entry of stratagem missile is analyzed according to thermal transfer theory, thermo-aerodynamics and N-S equation.

根据气动热力学、传热传质学、N-S方程,分析了战略导弹再入时端头帽烧蚀与碳颗粒的旋转效应,研究结果表明碳颗粒的旋转有利于防热。

My father is circumspective and bovine man.

父亲是个很细心很有耐心的人,记得我读初中的时候刚接触物理,偶尔遇到些题目(比如说是煤气炉,电饭煲等),父亲总是会不厌其烦地为我把它们&大卸八块&进行分析讲解,讲完还要在纸上把它们画出来再讲。

From the point of view of the framework, there is some language independency in CLAT, and it can not only adapt to different OO language, but also tell us that the feasibility and value of method of Concept Lattice.

从结构体系的角度看,该系统不仅具有一定的语言透明性,可以适用于不同的面向对象语言,而且证明了概念分析方法在遗产软件再工程中的可行性和实用价值。

The total RNA from brain tissue of substantia nigra of PD rats is extracted at the 1st, 2nd, 4th and 8th week and comparison is made between it and that from normal rats. The total RNA, after RNA formaldehyde degenerated sepharose electrophoresis and separation and Northern transfer, is adsorbed unto colloxylin membrane. Then ECL labeled Selenoprotein P and an unknown gene fragment are used as probes to hybridize with the RNA on the colloxylin membrane. And the hybridized result is obtained with enzyme-linked immunosorbent assay. Finally, analysis of OD is made using ONE-Dscan software.

分别提取1周、2周、1月、2月的帕金森大鼠(各8只)黑质区脑组织总RNA,并取相对应时间的正常大鼠的脑组织总RNA作为对照,经RNA甲醛变性琼脂糖凝胶电泳分离后,通过Northern转移,将其吸附在硝酸纤维素膜上,然后利用ECL标记的Selenoprotein P和一段未知基因为探针,与硝酸纤维素膜上的RNA分子进行杂交,再通过显影取得杂交的结果,应用ONE-Dscan软件进行光密度值分析。

Background— Mild commissural mitral regurgitation is associated with significantly higher restenosis-free surial after percutaneous mitral aluloplasty, which suggests that different mechanisms of significant MR after PM may hae different clinical courses.

背景 经皮二尖瓣成形术后轻度的联和部二尖瓣返流患者术后无再狭窄生存率明显较高,提示着成形术后不同机制的显著二尖瓣返流有不同的临床进程,因此对成形术后显著二尖瓣返流患者的长期预后因素进行了分析

Accordingly, analytic Shanghai again obtain employment project reachs his of relevant policy carry out, the development direction of the change of the situation of obtain employment of our country big city in conducing to understanding transition and labour market, conduce to make policy of proper labor obtain employment.

因此,分析上海市再就业工程及其相关政策的实施,有助于了解转型中的我国大城市就业形势的变化和劳动力市场的发展方向,有助于制定正确的劳动就业政策。

Above all, Husserl, Heidegger and Gadamer all address in the internal perspective of the confirmable knowledge, while philosophy of logic empiricism and holistic analysis do it in the external perspective No.

如果说从胡塞尔到海德格尔再到伽达默尔是从内在的视角探讨知识的确定性的话,那么,四、逻辑经验主义以及整个分析哲学所采取的则是外在的视角来探讨知识确定性的。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。