再分析

Methods Ampicillin inhibitionof E. coli Top10 pcs+ was tested at first, and then b-lactamase activity in periplasm was examined.

然后再使用抗生素抗性分析、b-内酰胺酶的酶活测定以及Western blot杂交技术，分析质粒编码的b-内酰胺酶从细胞质到细胞间质的分泌情况。

The studies on analysis of the product shew GC analysis had better stability and recurrence.Column temperature was set 100℃ for 1 min and was programmed at 16℃/min to 260℃,then 10℃/min to 330℃,last at 5℃/min to 370℃. Results of study on different catalyzers shew phosphotungstic acid was the best catalyzer.

产物的分析测试研究表明：GC分析比RP-HPLC法的稳定性和重复性好，适宜的操作条件为：柱温在100℃下维持1分钟，先以16℃/min升到260℃，然后以10℃/min升到330℃，再以5℃/min升到370℃。

For this reason, some basic studies, such as the definition of the environment, systematic analyses of the ecosystem of man-environment, deep thinking about environmental problems, navigative proposal of environmental construction, assessment of environmental awareness and ethics, comments on importance of morals and laws, philosophical ponderation of environmental values, and discussion on relationships between science, technology and the environment are previously conducted in the dissertation.

为此目的，本文首先通过对环境的基本概念的界定、人类-环境系统的层次分析、环境问题的理性透视、环境意识与生态伦理、环境价值的哲学思考、科学技术与环境、从环境保护到环境恢复再到环境建设等方面的讨论，向读者介绍了自己对环境的若干思想和认识，这也为之后的研究作了必要的铺垫。在上述讨论中，尤以经济与社会的层次分析、环境保护与环境恢复和环境建设战略辨析、环境道德与环境法制及环境价值的哲学反思等方面的论述最能体现作者独到的见解与理性。

This paper, based on the land utilization change data during 1997 to 2005, applies the information entropy and equilibrium theory to study the temporal-spatial changes in land utilization structure in Lingshui county, with results indicating: a first slow and then a fast rise in information entropy of land utilization structure in Lingshui county and a general trend of rising homogeneity in land utilization structure; the information entropy of land utilization structure ascends from NW to SE, where can be divided into five regions, ecological protected area, resort area, farmland protected area, ecological agricultural construction area and comprehensive industrial developing area; the economic factors and populational structure play a key part in land utilization structure changes via principle componential analysis.

基于陵水县1997-2005年土地利用变更数据，利用信息熵和均衡度的基本原理，对陵水县土地利用结构的时空变化进行实证分析。结果表明：1陵水县1997-2005年土地利用结构信息熵和均衡度呈现先缓慢增长、再加速增长的变化过程，总趋势是土地利用结构的均质性逐渐增强；2）陵水县土地利用结构信息熵从西北向东南逐渐递增，依次可分为生态保育区、风景旅游区，基本农田保护区、生态农业建设区和综合产业发展区5个区域；3）利用主成分分析，经济因素和人口结构对陵水县土地利用结构变化影响重大。

The analysis of adults, as well as of children, has familiarized us with the fact that the pregenital instinctual impulses carry with them a sense of guilt, and it was thought at first that the feelings of guilt were of subsequent growth, displaced back on to these tendencies, though not originally associated with them.

成人的分析，如同儿童的分析，使得我们熟悉与这样的事实，就是说前生殖器期的本能冲动给他们带来了一种罪恶感，并且首先被认为罪恶感是随着成长产生的，再转移到这些倾向之上，虽然并非一开始就和它们相联系。

In this study, the genomic structure and regulatory elements of ApoD from 10 representive species including protozoan, invertebrate, protochordate and vertebrate were analyzed and compared. The genomic structure of ApoD is less conserved in organisms from protostome to deuterostome invertebrates, while it is highly conserved among chordates including amphioxus and verebrates. All four conserved cysteine residues are present in amino acid sequence of deuterostome ApoDs, while there are only two cysteine residues in amino acid sequence of protostomes ApoD. Structure divergence between protostome and deuterostome ApoD proteins suggests their function difference. The majority of regulatory elements are present in nearly all organism ApoD genes ranging from unicellular protozoan to mammals, suggesting that ApoD plays a very fundamental role, and possesses a conserved regulatory mechanism. However, there also exist some specific regulatory elements, which are present only in certain species and may perform some special roles.ApoD mRNA expression in murine NIH/3T3 fibroblasts exposed to various stresses such as as hydrogen peroxide and UV light shows dose-dependent increase. And a fly homolog of ApoD, Glial Lazarillo, whose overexpression results in increased resistance to hyperoxia as well as a extension of lifespan under normoxia and resistance to starvation without altering lipid or protein content.

本文首先从生物信息学角度对分属于原生动物、无脊椎动物、头索动物和脊椎动物类群的10种动物ApoD的基因结构及调控区的调控元件进行分析及比较，发现：(1)ApoD基因外显子-内含子结构从原生动物草履虫到原口动物再到后口动物海胆的进化过程中不保守，但在分析的几种脊椎动物中相当保守；(2)文昌鱼ApoD基因扮演从无脊椎动物到脊椎动物承上启下的角色，可能代表了脊椎动物ApoD基因原型；(3)四个半胱氨酸保守位点在后口动物中都存在，而在原口动物中只存在两个，原口、后口动物ApoD蛋白一级结构上的差异反映蛋白功能上可能也存在一定差别；(4)调控区大多数主要调控元件为不同动物共有，说明ApoD主要功能及其表达调控在进化中相当保守；(5)ApoD基因个别调控元件是随着物种进化而出现并开始发挥相关作用，如SF-1；还有一些调控元件在进化过程中还没有发现其规律，这说明ApoD某些功能和基因表达调控模式可能因物种不同而存在一定的差异。

METHODS: The whole protome of H37Rv was scanned by SignalP and TMHMM. The protein date analysis system based on Visual FoxPro was established to process the output of SignalP and TMHMM and identify the secreted proteins. The sequences of the secreted proteins were aligned by BLASTp.

以SignalP和TMHMM两个软件对结核分枝杆菌蛋白组进行扫描，基于Visual FoxPro构建&蛋白质数据分析处理系统&对扫描原始数据进行分析处理以识别分泌性蛋白，再经BLASTp完成相似性比对。

aim: to establish a prediction strategy for mycobacterium tuberculosis (h37rv) secreted proteins to pave the way for further research. methods: the whole protome of h37rv was scanned by signalp and tmhmm. the protein date analysis system based on visual foxpro was established to process the output of signalp and tmhmm and identify the secreted proteins. the sequences of the secreted proteins were aligned by blastp.

以signalp和tmhmm两个软件对结核分枝杆菌蛋白组进行扫描，基于visual foxpro构建&蛋白质数据分析处理系统&对扫描原始数据进行分析处理以识别分泌性蛋白，再经blastp完成相似性比对。

Although the EPRI give a set of the approximate engineering solution method, actually the crack of parts in engineering project mostly presents inner 3D effect. If using 2D elastic-plastic or 3D elastic analysis, the result can't be precise. Because these methods have neglected the reassignation of the stress and strain which happened at nearly the top of cracks, carrying on 3D elastic-plastic analysis and J-integral calculation become the foundational work to study the expander and safety assessment of the pressure vessel cracks.

虽然EPRI给出了一套近似的工程解法，但实际上工程中构件的裂纹大多呈现内在的三维效应，若使用二维弹塑性或三维弹性分析必然不够精确，因为对裂纹的&等效&二维表达忽视了发生在靠近裂纹端部的应力和应变的再分配，所以对压力容器裂纹进行三维弹塑性分析及其J积分是研究压力容器裂纹扩展和安全评定的基础工作之一。

The unit of IPL Purify in IPL circulation system was calculated and analyse . Feed temperature is consequential to effect of extract and vapor flux of reboil is marked to the stripper.

对无机循环系统的无机工艺液净化单元进行了工艺参数分析，定量的分析了进料温度和进料量对萃取塔萃取效果的影响，以及再沸器蒸汽流量对汽提塔分离效果的影响。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。