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再分化

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The regeneration plant of Dianthus chinensis L. was obtained by two ways of the leaf callus redifferentiation and the adventitious buds induced directly by lamina.

方法通过叶片愈伤组织诱导再分化和叶片直接诱导不定芽2种途径获得了石竹的再生植株。

It is vital to reduce the variation of Dracaena fragrans cv.massangeana and Dracaena fragrans cv.lindenii by preventing the differentiation from forming callus as well as the reduction or the prevention of callus redifferentiation.

避免脱分化形成愈伤组织以及减少或阻止愈伤组织再分化是减少金心也门铁和金边也门铁这类嵌合体品种发生变异的关键之一。

The results show that NAT can effectively delaies the senescence of flag leaves and promotes both the development of roots of cutting leaves of hollyhock and the redifferentiation of young spikes of wheat culture in vitro, and also indicate that the effect of NAT is more remarkable than that of NAA.

结果表明,NAT可有效的延缓小麦旗叶的衰老,促进蜀葵叶柄的扦插生根和促进小麦幼穗离体培养的再分化作用。其效果比NAA更为明显。

In resting cysts, the microtubules under the pellicle, cortical ciliatures, conjunctions between cilia basal bodies, supporting structure of basal bodies and other microtubule structures are absorbed in different extent; microtubule-like assemble bodies appear at the center of the remained cilia basal bodies; the granules of chromatin and nucleolus in macronucleus become smaller whereas nucleus pores of the micronucleus become bigger; the inner membrane of some nucleus pores are attached with chromatin.

结果表明:纤毛虫形成包囊过程中,皮层细胞质中产生具有自噬泡消化特征的细胞器,将部分纤毛结构等逐步消化;休眠包囊中,表膜下微管、皮层纤毛器和纤毛基体间连接、基体托架及其他微管结构不同程度地被吸收、在残剩纤毛基体的中央形成微管形结构聚合体,大核染色质和核仁颗粒变小,核孔增大,并发生染色质附着于核孔内膜的现象;细胞脱包囊期间,皮层细胞骨架、线粒体等经历了在残剩结构基础上的再分化

Effects of mannitol and 6 BA on the rice cell redifferentiation,activities of peroxidase and IAA oxidase were investigated.

研究了甘露醇和 6- BA处理对水稻悬浮细胞再分化、过氧化物酶及 IAA氧化酶的影响。

Result and Conclusion:All the media used could induce callus, while the results of callus redifferentiation were very distinct.

结果与结论:所用培养基均能诱导愈伤组织产生,但愈伤组织形成后的再分化结果却有明显差别。

The research progress of redifferentiation of dedifferentiated thyroid carcinoma by up-regulation of thyroid iodide-metabolizing genes expression and suppression of BRAF mutation is reviewed in this paper.

文章就通过上调甲状腺碘代谢相关基因表达和抑制BRAF基因突变而使失分化型甲状腺癌再分化的研究进展作一综述。

After dedifferentlation and redifferentiation,the explants had three fates: abnormal plantlets, death and healthy plants.

外植体通过诱导经历脱分化和再分化,最后大致有三种去处:诱导出畸形苗,死亡和诱导出正常植株。

In inducing callus process, the suitable illumination conditions were the former period with darkness culture and the later period with light culture. In inducing dedifferentiation and redifferentiation process, the temperature of 24-26℃ was suitable.

在愈伤组织诱导时,光照条件以前期暗培养、后期光培养为宜,在诱导脱分化和再分化时,培养条件以温度24-26℃为宜。

No callus could be induced from leaf or petiole explants of varieties F30 and Ⅱ cultured on MS medium if supplemented alone with 6-bezyladenine (6-BA) or thidiazuron, or kinetin. However, the calli were induced on MS medium containing 2.0 mg L-1 6-BA and 0.5 mg L-1 NAA (α-naphthaleneacetic acid), and the calli derived from petioles of variety Ⅱ could produce redifferentiated shoots, but of variety F30 could not. The later could only produce shoots directly from the base of the petioles.

将品种F30和Ⅱ的叶片和带叶叶柄接种到含不同浓度单一细胞分裂素(6-BA、TDZ、KT)的MS培养基上培养,均未能诱导出愈伤组织,而在含2.0mgL-1 6-BA+0.5mgL-1 NAA的培养基上可以诱导出愈伤组织,且由品种Ⅱ叶柄诱导产生的愈伤组织可再分化出芽,品种F30诱导的愈伤组织却不能分化,但在其叶柄基部可直接出芽。

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