再冷冻

This paper develops the method of extraction and purification of thrombin from the porcine plasma by using Ion Exchange.

对离子交换法提取纯化凝血酶的方法进行了改进：首先在-20℃对猪血浆进行冷冻处理，然后再采用离子交换法提取纯化凝血酶。

Pour the mixture into the piecrust , then the mixture , swirl the top to create a muddy look , chill for 2~3 hours until set.

先将的混合物倒入派里，再倒入的混合物，轻轻搅拌成泥浆状，冷冻约2～3小时。

Refined snake venom is first produced with snake venom of Pallas pit viper through twice chromatographic separation, saturated ammonium sulfate precipitation, centrifugal separation, dialysis and freezing drying and then produced into the said medicine through compounding, filteration, disinfection and packing.

一种治疗肝炎病的药物疏肝酶，是由复蛇蛇毒经两次层析分离，饱和硫酸铵沉淀，离心、透析、冷冻干燥而得精制蛇毒，然后再经配制、过滤、除菌灌注、封口而成。

Promoting Prunus Humilis Bunge Industrialization to Protect Ecological Environment;2. This article is about the ratafia of Prunus humilis bunge of dry and sweet which has the special fragrance and flavor of Prunus humilis bunge .

研制开发具有欧李果独特的香气和风味的干型和甜型欧李果酒，通过对欧李果的特点研究，确定了果实中度破碎，18℃￣22℃下低温浸皮发酵，18℃￣20℃"苹果酸-乳酸发酵"，再经调配、冷冻、0。

Unroasted black sesame seeds were pressed and defatted with n-hexane, extracted with 80 % methanol, and separated by XAD-2 gel open column chromatography eluting with a serious of concentrations of aqueous methanol solutions (20 %, 40 % and 60 %). The 60 % methanol eluate, which contained higher content of ST compound, were collected and freeze-dried to obtain the ST extract powder.In animal experiment, we used vitamin E deficient rat model to increase oxidative stress.

试验材料为未经炒焙之泰国黑芝麻，将脱油后之芝麻粕先以80 %甲醇溶液进行萃取，经浓缩后再以XAD-2胶体进行管柱层析，以不同浓度之甲醇水溶液冲提后，收集ST含量较高之60 %甲醇水溶液区分层，行冷冻乾燥可得ST萃出物粉末。

Mandibles devitalized by freezing and boiling after being removed from animals were reimplanted to evaluate their potentials of reunion and growth with X-ray and histologi-cal methods.

本文通过动物实验，采用X线片和组织学检查方法观察比较冷冻和煮沸自体下颌骨再植术后的骨愈合过程。

Nepalensis wall polysaccharide was prepared with hot water extraction,ethanol precipitation,dialyzation,and lyophilization.the crude polysaccharide was separated by deae-cellulose chromatography and purified by sephadex g-200 chromatography.the inhibit effect of the polysaccharide on lipid peroxidation of mice liver was conducted by tba method.the model of liver-injury induced by carbon tetrachloride（ccl4was used to validate the variety of the realated ast.results onefold ingredient polysaccharide was obtained after isolated and purified.conclusion the ingredient can remarkably inhibit lipid peroxidation of rat liver and significantly reduce activity of ast.

用热水提取，乙醇沉淀提取粗多糖，再经sevag法去除蛋白，透析去除小分子物质，冷冻干燥得楮头红多糖粗品；采用deae-52纤维素柱层析和sephadex g-200凝胶柱层析进行分离纯化；用tba法考察多糖的抗脂质过氧化作用；采用ccl4急性肝损伤模型，测定其血清中ast变化。结论获得一个楮头红多糖组分。tba法表明该组分对小鼠自发性脂质过氧化具有显著的抑制作用；在保肝试验中，给药组能明显抑制ast的升高，对肝损伤有一定的保护作用。

Store up test result makes clear, when the freeze-dry bacterium pink that adds 1% cane sugar to serve as protective agent, be in respectively rise and fall normal temperature (6~33 ℃) and constant microtherm (4 ℃) next storing up after 18 months, make with fluid of amortize of 0.05 Mol/L phosphoric acid again to hydrate agent, TW surviving rate can be amounted to respectively 70% with 90% above.

贮藏试验结果表明，当添加1%蔗糖作为保护剂的冷冻干燥菌粉，分别在起伏常温(6~33℃)和恒定低温(4℃)下贮藏18个月后，用0.05 mol/L磷酸缓冲液作再水化剂，TW存活率分别可达70%和90%以上。

Methods:The artificial defects of 20 rabbits were repaired with frozen allogeneic mandible and fresh syngeneic mandible to study the revascularization's dynamic process of mandible transplantation by the inkgelation vascular perfusion,vascular corroisioncast,Nuclein scintigraphy;the 85 cases received frozen allogeneic mandible transplantation and the measures to improve revascularization;The defects were implanted with bFGF and allogeneic bone,the defects were only repaired with allogeneic bone as control group.

通过冷冻异体下颌骨和新鲜自体骨移植修复20例家兔下颌骨缺损的动物实验，经墨汁明胶血管灌注法、血管铸型法、放射性核素骨显像研究了异体下颌骨移植再血管化及骨改建的动态过程；同时另20例采用bFGF和异体骨修复作为实验组，单纯异体骨修复作为对照组，植入后1个月、3个月行墨汁明胶血管灌注透明法及组织学观察。

Two pairs of primers (TS5/TS11 and S18/S19) were used for the PCR method. Positive PCR results were obtained after the 18-hour preculture (6 h in lactose broth at 37℃ followed by 12 h in Rappaport-Vassiliadis broth at 42℃) for spiked raw and irradiated meats with or without freezing treatment.

试验以经过或未经过冷冻处理之加菌的鸡绞肉及辐射鸡绞肉，采用乳糖培养液於37℃培养6 h，续以Rappaport-Vassiliadis 培养液於42℃培养12 h之预培养，再配合PCR法，均可检测出沙门氏杆菌。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新