再冷冻

Objective: To study the feasibility of reserving the teeth in the transplantation of frozen autogenous mandible.

目的：研究冷冻自体下颌骨再植术时保留颌骨中牙根的可能性。

From there, use of boxcar cow to Chicago, after slaughtering, cutting and freeze and then transported to the East.

从那里，用棚车把牛运至芝加哥，经屠宰、切割和冷冻后再运往东部。

University of Central Florida biomedical scientists led by Professor Henry Daniell found insulin might someday be grown in genetically modified plants and then be used to prevent diabetes before symptoms appear or to treat the disease in its later stagesDaniell's research team genetically engineered tobacco plants with the insulin gene and then administered freeze-dried plant cells to five-week-old diabetic mice as a powder for eight weeks.

在弗罗里达中央大学，由Henry Daniell教授带领的生物医学学家们发现胰岛素可以在转基因植物中生产，以用来在症状出现之前预防糖尿病或在疾病晚期治疗糖尿病。Daniel的研究小组将胰岛素基因通过基因工程技术重组到烟草植物之中，再将经过冷冻干燥过的植物细胞用作粉剂喂食五周大的糖尿病鼠8周。

The release in vitro of insulin from Ins-NC can be described by double exponential equation.

研究表明注射用胰岛素纳米囊具良好的外观及再分散性，冷冻干燥前后平均粒径及包封率几乎无变化，初步稳定性实验表明，注射用胰岛素纳米囊在4℃及室温较稳定，含量，包封率，平均粒径等无变化，体外释药符合双指数模型。

The invention uses the fermentation liquor of one Escherichia coli generating polysialic acid as material, filters and removes thallus, adds some solvents as sodium chloride and alcohol into supernatant to deposit polysialic acid, dissolves the polysialic acid deposits via deionized water, filters to remove modified protein, uses alkali prolease to treat filtrate, complexes deposits via cetyl pyridinium chloride or cetyltrimethylammonium bromide, and dissociates, uses over alcohol to deposit polysialic acid, dissolves the deposits via ultra-pure water, grades and purifies via chromatography column, collects and dialyzes eluent, cools and dries to obtain polysialic acid.

本发明以一株产聚唾液酸大肠杆菌的发酵液为原料，经过过滤去除菌体，上清液加入适量氯化钠和乙醇等溶剂沉淀出聚唾液酸，聚唾液酸沉淀用去离子水溶解，再用过滤去除变性蛋白质，滤液经过碱性蛋白酶处理、氯代十六烷基吡啶或十六烷基三甲基溴化铵络合沉淀并解离后，再用过量乙醇沉淀出聚唾液酸，聚唾液酸沉淀用超纯水溶解，用层析柱进行分级纯化，收集洗脱液经过透析，冷冻干燥得聚唾液酸产品。

By partially thawing the prefrozen samples and refreezing them in liquid nitrogen before the final liyophilization, we prepared biomimetic multilayered scaffolds.

采用-20℃冷冻后，部分熔融再液氮重冻的预冻方式，冷冻干燥制备了厚度约2mm的仿生多层软骨支架。

Acid treated corn starch-water suspensions (5%) were subject to high pressure treatment at 500 MPa for 15 min. The samples were dried after being immediately treated, retrograded for 3 d (4℃), freezed (-20℃) for 24 h and retrograded for 3 d, and then freezed and dried.

将酸解后的玉米淀粉配制成质量分数5%的淀粉悬浮液，在500 MPa压力下处理15min，然后分别立即处理、回生3 d(4℃)、冷冻(-20℃)及回生3 d后冷冻处理，最后再进行干燥处理。

In early days, there were two kinds of Old-fashioned Clear Ice. One is to sprinkle the syrup onto the shaved ice; the other kind is to shave the frozen ice brick, which is a mixture of boiling water, white sugar and banana oil, into snow-like tiny pieces.

老式清冰有两种，一种是将冰砖刨成雪花状后再淋上糖水；另一种则是将开水煮沸后加入白砂糖及香蕉油，搅拌后冷冻成冰砖状，再刨成雪花状的冰品，尝起来有股清新的果香与微甜口感。

Loofa obtained from traditional market was cut into approximate 1 cm in length and then treated with 1 N sodium hydroxide at 85 oC for 4 hours. The pulp like suspension was bleached by 25 % sodium hypochloride until the fiber turned colorless and the pulp was washed by distilled water to remove excess hypochlorite. The white pulp was again filtered and lyophilized to make into a paper-like membrane.

将购自传统市场之菜瓜布，裁剪出大约1公分长之纤维，再於浓度1 N的氢氧化钠之溶液加热至85 ℃处理4小时，以25 %之漂白水将此纸浆状之悬浮液漂白，使其纤维褪色后再以蒸馏水清洗以去除次氯酸盐，最后将此白色悬浮液过滤及冷冻乾燥，制成纸状的薄膜。

The coal tar pitch powder was used with each of 22 kinds of dispersants prepared coal tar pitch water slurry,and 6 kinds of dispersants were selected to prepare coal tar pitch water slurry with the high temperature coal tar pitch powder which has a softening point of 105℃.

采用冷冻粉碎的方法将软化点为90℃的中温煤沥青制得粒度≤200目的煤沥青粉后，再与22种分散剂制备煤沥青水浆，从中筛选出6种成浆性好的分散剂再与软化点为105℃的高温煤沥青粉制备煤沥青水浆，结果表明中温煤沥青粉比高温煤沥青粉更易制得稳定性好的煤沥青水浆。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。