再冷冻

This paper introduces the food refrigerated theory and the basis of the current industry refrigerated developments in the situation; And then in the long cylindrical-shaped food heat conduction process and the mathematical model using the method of differential thermal conduction equations for discrete, Programming come through long cylindrical-shaped food cooling process numerical solution; After further analysis of numerical solution of the test results; Finally, the impact of the cooling process food all relevant factors, such as moisture content, wind speed and temperature of the cooling medium for in-depth research, while comparing the different shapes of food to food cooling process.

本文首先介绍了有关食品冷冻冷藏的基础理论和目前冷冻冷藏行业的发展的发展概况；然后研究了长圆柱状食品热传导过程的数学模型并利用差分的方法对热传导方程进行离散，通过编程得出长圆柱状食品冷却过程的数值解；之后再分析解对数值解的结果进行验证；最后对影响食品冷却过程的各相关因素，如含水率、风速和冷却介质温度等进行了深入的研究，同时又对比了不同形状的食品对食品冷却过程的影响。

Researchers have had systematically studied on this technique for many years, and this technique has been utilized in application and made a lot of effects;2 Combining with the techniques of cell engineering (nuclear transplantation and electric fusion etc.), and through the process of culturing histiocyte of fish, cryopreservation and re-culture after thawing, carrying out somatic cell breeding of fish.

多年来，国内外学者对各种鱼类精液的冷冻保存进行了大量的系统研究，目前这项技术已达到实用水平，并日益发挥作用；二是对鱼类培养的组织细胞冷冻保存，通过鱼类细胞的培养、超低温冻存、解冻后再培养过程，结合细胞工程技术进行体细胞育种；大量的研究结果表明鱼类体细胞核具有发育的全能性，随着细胞培养技术、细胞工程技术日益发展成熟，完全具备实现鱼类物种种质长期保存的理论基础和技术条件。

The platelet count, aggregation functions, adhesive ability, hypotonic shock reaction and CD62p expression of platelets preserved at normal temperature, cryopreserved on same day of collection and after storage for 3 days were detected, and possibility of using platelets cryopreserved after storage for 3 days in clinic was evaluated by comparison of contractable clinical cases.

对当天冷冻和保存3天后再冷冻血小板进行了计数，并检测聚集力、黏附力以及CD62p的表达，并通过可对比性病例观察保存3天后再冷冻与当天冷冻血小板临床应用的可能性。

After that, the comparison of the fertilizable competences of ICSI and IVF on cryopreservated oocytes was studied in this article, in order to establish an optimized method to improve the fertilizable competences of oocytes up to the level of embryos.1 Effects of the reducing injury methods on IVF of cryopreservated oocytesThe result of the three methods, choosing the right time oocytes, using minimum size sample vitrification and culturing after thawing, showed that these ways could partly maintained the fertilizable competences of cryopreservated bovine oocytes.

减轻损伤方案对冷冻卵母细胞IVF的影响使用选择合适时期的卵母细胞进行冷冻；采用最小样本量玻璃化冷冻方法进行冷冻；在解冻后先孵育再进行体外受精等三种方案能够部分维持或恢复冷冻牛卵母细胞IVF受精能力。

The steps are as the following:1、Preparing the cerebrospinal fluid one hour before the experiment,take some of them for cryopreservation;2、Without paralyse,cuting down the rat head quickly and dislodge the hippocamp part which is used in the experment in freezing liquid,and trim the hippocamp distrct.3、Fixing the hippocamp tissue on the vibratome,to cut down a brain slice with 300μm,use the haustorial tube to move the slice to the preincubate dish,cultivanting it for one hour.Then move it to the record incubation chamfer.4、Preparing the glass micloelectrode and fill it with NaCl,the corcentation is 3mol/L.5、adjusting the recorder,after cultivate the brain slice in the incubation chamfer for 2 hours,the expermentize can be gone on.6、In order to ensure the accurate of the experimental result,use only one medicine concentration for one brain slice in every experiment.

本实验研究方法是：1、实验前1小时配制好所需脑脊液并充以95% O2和5% CO2的混合气，取小部分冷冻保存；2、在乙醚麻醉下，快速断头并在冷冻液里取出包含海马的大脑部位，修整出所需海马区；3、将海马组织固定于振动切片机上切出300μm厚的海马脑片，用广口吸管转移至预孵育皿培养一小时，后再转移到记录用孵育槽内；4、拉制好玻璃微电极，并充以3mol/L的NaCl；5、调试好记录仪器，将脑片在孵育槽内培养2小时后进行实验；6、为了保证实验结果的准确性，每一块脑薄片只进行一个浓度的药品实验。

METHODS: 15 g/L silk fibroin solution was made by degumming, salvation and dialysis. Chitosan was dissolved in 2% acetic acid solution to prepare 25 g/L chitosan-acetic acid solution. Two solutions were mixed to prepare six silk fibroin/chitosan solutions, and mass ratio was 10: 0, 5: 5, 4: 6, 3: 7, 2: 8, 0: 10. These solutions were separately sucked in a 24-well plate. Following exhausting gas vacuole at 4 ℃, precooling was performed at -20 ℃ for 12 hours, followed by cryodesiccate for 30 hours.

通过脱胶、溶解、透析这3个主要步骤制成质量浓度为15 g/L的丝素溶液，将壳聚糖溶解在2%的醋酸溶液中制成25 g/L的壳聚糖-乙酸溶液，然后将两者混合，配制成丝素/壳聚糖质量比分别为10∶0，5∶5，4∶6，3∶7，2∶8，0∶10的6种溶液，分别吸入24孔板中，4 ℃排出气泡后，-20 ℃预冷冻12 h，再冷冻干燥30 h。

Q. If frozen meat has defrosted, can it be refrozen?

问：如果冷冻肉被解冻，还可以再冷冻吗？

Ii eliminate unsafe handling practices for meat, poultry and fishery products which involve thawing of frozen bulk packages followed by repackaging and refreezing

排除大量冷冻包装解冻之畜肉及禽肉与鱼类及鱼类产品及之后再包装及再冷冻时之不安全处理行为。

In this paper, the changes of frozen doughs' temperature during freezing-storing and thawing were studied, and the better technology condition of freezing storing and thawing were selected.

研究了冷冻面团在冷冻贮存和解冻过程中面团温度的变化，并选择了冷冻面团面包的最佳冷冻贮存和解冻的工艺条件：面团 30℃、90 %～ 95%rh前发酵 2 0min ，成型后于- 2 0℃冷冻贮存；冷冻面团可在打开包装后，38℃，95%rh解冻 4 0min ，32℃，95%rh醒发 55min ，或用微波炉解冻档解冻 1min再打开包装，32℃，95%rh醒发 95mi

It looks to have a little ring of refrozen melt around it.

它看起来有一个再冷冻融化它周围的小戒指。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。